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phreak07
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1 Week, "In Vitro," No Pinning
#16098639 - 04/16/12 05:59 PM (12 years, 8 months ago) |
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So, inoculated these three tupperware bowls on like 3/20 or so. Did a shake, and by 4/8 two of them were fully colonized. Took them out, dunked them, rolled them, set them on lids, put bowl back over lids with some holes in it for FAE, put under fluorescent light. Plan is to fruit these IN the tupperware bowls, in vitro style.
It's been a week. I didn't think I should spray them because water collected inside the bowls, clinging to the outsides of the cakes. They both grew mycelium over the verm from the roll, both look pretty fuzzy in areas and damp on the outside.
 
However, it's now been a week and there's no pins. As I read in another thread (and not elsewhere despite days of research), I maybe was supposed to let them "consolidate" first, so I left the third bowl in a drawer to do this after it was fully colonized by 4/10. That one also doesn't have pins and is just white all over (as seen below).

I know that people say FAE and misting is important, but there's an entire thread on Mycotopia of growers who grow In Vitro, which involves no misting (and as far as they describe it, not even much FAE except the holes in the jar lids). I added more holes to the bowls, thinking of buying a mister to do that, and they're sitting on a rack to get indirect sunlight instead of a bulb.
Any input other than "Wait" and "Needs more humidity"? I don't know how I can add more humidity without just soaking their outsides at this point.
In other news, here are some jars that were inoculated with a pretty bad Spores101 syringe on like 3/10. They're probably a week away from full colonization, making it in total like 6 weeks. A side-effect from this super slow growth is these things have a TON of the yellow metabolites or whatever that stuff is -- kind of gross (I know it's not contams, it shows up as wet specks in the middle of growth on all the jars). I plan on putting these inside quart jars to fruit (gonna try to get a drill from a friend to just put holes in the glass for FAE).

Edited by phreak07 (04/17/12 12:57 AM)
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hardcoresmexyou


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Re: 1 Week, "In Vitro," No Pinning [Re: phreak07]
#16098660 - 04/16/12 06:06 PM (12 years, 8 months ago) |
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u have to allow consolidation
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phreak07
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Yeah, I figured, which is why I let the other cake rest. Is like 6 days of consolidation good? I remember the PF website says to wait until pinning starts to fruit, which I forgot, but there still isn't pinning in the last bowl.
Also, these will start fruiting eventually, right? Is there anything I should do to help that? After reading about consolidation, I figured that happens whether you leave them in the bowl or not (I assume that means allowing the fungus to settle all the way into the cake).
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hardcoresmexyou


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Re: 1 Week, "In Vitro," No Pinning [Re: phreak07]
#16099844 - 04/16/12 10:31 PM (12 years, 8 months ago) |
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.
Edited by hardcoresmexyou (05/02/12 09:03 AM)
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Divinity
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The cakes should be completely colonized. If you feel the need, let em sit an extra week once fully colonized to be sure(optional) What are you using for a fc?
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phreak07
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Re: 1 Week, "In Vitro," No Pinning [Re: Divinity]
#16099997 - 04/16/12 11:03 PM (12 years, 8 months ago) |
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As I explained in the first post, I'm using the containers themselves to fruit in using the "in vitro tek." Since I couldn't afford all the resources for an FC, I went into this intending to use an in vitro method with jars, but those jars ended up not working so well, so with these three containers I was gonna try a play off of that.
Because there is a lot more space in these bowls, it leaves room for the cakes to fruit without making it as cramped as the normal in vitro method does. However, I didn't read much about humidity/FAE in this tek, I'm not totally sure if the insides of my containers aren't humid enough -- I know some people grow in vitro without even opening the jar until the first flush is finished.
I think I'm gonna let the un-dunked one sit a bit longer. Luckily the humidity and heat just went up a lot outside/inside, so leaving these two containers out to get indirect sunlight and stay warm will hopefully help.
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Kizzle
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Re: 1 Week, "In Vitro," No Pinning [Re: phreak07]
#16100363 - 04/17/12 12:51 AM (12 years, 8 months ago) |
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You have the lid off right? That really is the main thing you can do to help speed up pinning. You could give it direct sunlight, just for a few minutes. That can help trigger pinning as well. As long as the verm is moist the humidity should be adequate.
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RogerRabbit
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Re: 1 Week, "In Vitro," No Pinning [Re: Kizzle]
#16100554 - 04/17/12 02:20 AM (12 years, 8 months ago) |
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Quote:
I know that people say FAE and misting is important, but there's an entire thread on Mycotopia of growers who grow In Vitro, which involves no misting (and as far as they describe it, not even much FAE except the holes in the jar lids).
I made a shitton of invitro posts over there when I was a mod ten years or more ago. Growing invitro will work, but it's far slower than using a fruiting chamber and yields are far lower. Invitro works much better if coffee is added to the substrate. Did you?
I'd suggest you pony up $20 for a terrarium setup. You'll be glad you did. Here's some invitro mushrooms. RR
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phreak07
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Re: 1 Week, "In Vitro," No Pinning [Re: RogerRabbit]
#16101258 - 04/17/12 09:25 AM (12 years, 8 months ago) |
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Hm, I was worried about that, which is why I've tried improving on it a bit. I felt the tupperware would create a sort of mini terrarium environment, and if there was enough FAE, it might work better than the normal in vitro set up.
That's also why I got some quart jars. I felt like a terrarium set up was a lot because most people don't just get the bins, they also get heating and humidity set-ups, which down the line I'd like to do, but for this time around wasn't that important (because from what I read this method worked, and it sounded more expensive). For those jars, I'm gonna try to get a drill that can put holes in the sides of these quart jars, then rest the cakes on the lids with the jars upside down.
Well, not a hugely successful experiment thusfar, but I've learned. When I've got a more secure living space and some money to put into it, I'll probably try a bit harder to go all out on the gear.
Edited by phreak07 (04/17/12 09:31 AM)
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RogerRabbit
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Re: 1 Week, "In Vitro," No Pinning [Re: phreak07]
#16101639 - 04/17/12 11:12 AM (12 years, 8 months ago) |
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Quote:
phreak07 said: I felt like a terrarium set up was a lot because most people don't just get the bins, they also get heating and humidity set-ups, which down the line I'd like to do, but for this time around wasn't that important (because from what I read this method worked, and it sounded more expensive)
No, most people spend less than 20 dollars to build a shotgun terrarium which will outperform all those silly 'automated' things.
You can use quart jars as a fruiting chamber too. Trivia: The picture below was part of my first post on shroomery. Gosh, I was still a moderator over at 'topia at the time. How things change.  RR
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phreak07
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Re: 1 Week, "In Vitro," No Pinning [Re: RogerRabbit]
#16102859 - 04/17/12 05:15 PM (12 years, 8 months ago) |
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Would you suggest putting the cakes in the quart jars that direction (in the bottom of the jar with the lid off)? I totally hadn't thought of that because the one quart jar tek I saw had it the other way around (using a weird PVC pipe stand to keep the holes in the lids open), but it would be a ton easier than getting a drill that can put holes in glass for FAE.
I didn't realize the shotgun FC was so cheap, I'll definitely go that route next time.
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phreak07
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Re: 1 Week, "In Vitro," No Pinning [Re: phreak07]
#16110846 - 04/19/12 09:43 AM (12 years, 8 months ago) |
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Hm, I have a blue spot on top of one of the cakes. A pool of water had collected there, so I tried trying it off with a paper towel. That spot was discolored, and I assumed it might be bruised. However, now it's kind of fuzzy and still discolored -- does that sound like bruising, or mold? I was worried that something bad might have been growing in the pool of water.
Sorry for no pictures. Some people talk about having some verm with water on top of the cake, and none of them say that causes mold to grow, but I don't know.
There's also STILL no pins, almost 2 weeks now. They're definitely getting plenty of fresh air, so would you say it's not enough humidity in the bowls? Water collects on the outsides of the bowls every day, so I assumed there's got to be some evaporation going on inside. I could try spraying them, but I feel like they'd get really wet -- also, there's a lot of holes in these for FAE, so I doubt it's that.
Edited by phreak07 (04/19/12 09:48 AM)
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hardcoresmexyou


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Re: 1 Week, "In Vitro," No Pinning [Re: phreak07]
#16110867 - 04/19/12 09:49 AM (12 years, 8 months ago) |
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can you possibly post pics?
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phreak07
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Here:

I've read I can put peroxide or something on that if it's mold?
You can also see that the tops of them are pretty speckled with water. Does that fulfill what humidity does, or do I need to make the insides warm enough that the water stays in the atmosphere instead of condensing?
Edited by phreak07 (04/19/12 10:04 AM)
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MagicCarpetRide89
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Re: 1 Week, "In Vitro," No Pinning [Re: phreak07]
#16110936 - 04/19/12 10:12 AM (12 years, 8 months ago) |
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that looks like it could be trich. But I could be wrong. How long has that spot been like that at the top? And is it aggressive?
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phreak07
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Well, it just became fuzzy like that in the last 24 hours. Before, it was just a blue spot on the surface of the myc for at least 4 days.
Can I do anything to get rid of it? I've read salt or peroxide?
Edited by phreak07 (04/19/12 10:47 AM)
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MagicCarpetRide89
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Re: 1 Week, "In Vitro," No Pinning [Re: phreak07]
#16111095 - 04/19/12 10:57 AM (12 years, 8 months ago) |
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I could be wrong like I said, so just wait to see if someone else chims in. If you don't hear anything from anyone else by tonight, either you can wait to see if that spot turns green or chance it and wait to see if it does pin. Either way, I hope you have that cake away from your other ones. Don't want to spread the love from that one!!
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phreak07
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Is it an urgent issue? Like, if I leave this for too long in the next few days, will I lose the whole cake? Can I afford to wait to see if it gets worse?
And if it is a contam, are there different removal techniques for different contams?
I think it probably is, the edges around it are brown and the surface is a different overall texture than the myc.
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Synapse Trap
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Re: 1 Week, "In Vitro," No Pinning [Re: phreak07]
#16115002 - 04/20/12 04:40 AM (12 years, 8 months ago) |
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I'm a bit confused on the drilling of the glass.. would your cakes be in it while you drill? couldn't you get glass shards all in your mushrooms that you will eventually be swallowing?
As RR mentioned, a Shotgun Fruiting chamber is nothing more than a plastic tote with some perlite in the bottom (and holes drilled all over it) heck you might even have a suitable tub laying around the house.
I'm not even certain it would have to be a clear tub, you could probably hang a light from the lid, just make sure it's a bulb that is 1. at or near 6500K 2. can handle humidity/condensation.
I saw one set up where a guy used outdoor Christmas LED lights inside the lid. Gotta get creative..
aside, once you drill the jars, aren't they useless after that one grow?
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phreak07
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Re: 1 Week, "In Vitro," No Pinning [Re: Synapse Trap]
#16115466 - 04/20/12 08:36 AM (12 years, 8 months ago) |
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Uh, no, the drilling glass is an aside and not related to this thread really.
The jars would be upside-down, lids down. These are quart jars, not the jars I inoculated in (over twice the size). I'd drill holes in the "bottom" of the jar before putting the cakes in (obviously). Then the quart jar is a mini fruiting chamber. However, as RR showed, I can just put the cakes in with the jar right-side-up and put holes in the lid.
Edited by phreak07 (04/20/12 03:42 PM)
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phreak07
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Re: 1 Week, "In Vitro," No Pinning [Re: phreak07]
#16116772 - 04/20/12 03:47 PM (12 years, 8 months ago) |
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Well shit, it's definitely like trich I think. The thing has turned green and is slowly spreading.
This happened because there was water collected on top of the cake. I remember someone suggested letting that happen, but I would say it's a bad idea. Within a couple days of it resting there, this contam developed in the little bit of water.
The good news is the third cake is really healthily colonized, really thick white myc all over it. I just dunked it for 12 hours and put it back in its fruiting bowl. The two good ones are sitting on top of my PC for some minor warmth, which should hopefully facilitate humidity and FAE.
[EDIT] Wooooo!!! The first non-contammed cake has tiny pins on it.
My 7 healthy PF tek jars should finish colonizing in a week or two, making for a full 8 weeks. Due to some moving issues, probably gonna have to just let 'em wait in the jar until there's a good situation to fruit them in, but shouldn't be a big deal.
Edited by phreak07 (04/20/12 04:46 PM)
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phreak07
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Re: 1 Week, "In Vitro," No Pinning [Re: phreak07]
#16182205 - 05/04/12 03:14 PM (12 years, 8 months ago) |
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So I got a couple good fruits off of one of these cakes, but trouble is brewing now.
On the cake that fruited has a little chunk on the bottom with like 8 pins. They've stayed the same size for weeks now, but only one of them has a remotely dark top. I dunked them as is, but they're STILL not growing. They look healthy and red/brown, but they're not doing anything (opening up, growing, etc). Is this normal? I don't feel like I've heard of this before. They definitely have not aborted the way four other pins on top did.
I have another cake that was REALLY well colonized, really thick myc, and it's been sitting in the FC (which is the tupperware things) for like 2 weeks now with no pins. There's some spots on its surface that are brown, some that are slightly blue, and then others that are fuzzy and moist. There's little knots of myc, but they're not turning into pins or anything.
The way I've been trying to fruit these is that they're in tupperware bowls with a lot of holes for FAE. Then they're resting slightly on top of a computer fan that keeps the temperature warm (I'd say 70 to 80). I put water in the lids of the bowls that evaporates inside from the warmth so that it's humid. This led to a couple fruits growing on one, but now neither of them are doing anything.
Any suggestions?
Edited by phreak07 (05/04/12 03:16 PM)
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