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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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Re: Do you think this is possible ? [Re: growin]
    #1626356 -

Quote:
I'M random... 



Apparently so.... :wink:

--
Micro 


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)

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Re: Do you think this is possible ? [Re: micro]
    #1637138 -

Quote:
As for other types of mushrooms, though, there has actually been some success in splicing hyphal cells' genetics between two types of fungi using polethylene glycols; this would be cheap. Just don't know the success rate.



Can you elaborate on that a little?


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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Re: Do you think this is possible ? [Re: mycofile]
    #1638283 -

I'm looking at the textbook, I think.... Might take a little bit.

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)

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Re: Do you think this is possible ? [Re: micro]
    #1638669 -

Turns out it's actually protoplast fusion, so it isn't as easy as I thought....

I just read the whole damn chapter on genetics, again, and of course I couldn't find it.  Prolly wasn't even the right book :wink:

It made me mad, though, so I did an extensive search, and after a while found this:

http://fungus.org.uk/cv/thesis_fig5.4.htm

and another reference not used in that thesis, if you want (sounds very relevant:)

Ogawa, K. 1993. Interspecific
and intergeneric hybridization of edible mushrooms by protoplast fusion.

While reading the chapter again, however, I came across an interesting piece of info related to mating 2 incompatibale isolates from the same "parental strain."  Take the two, make them each nutritionally deficient for a seperate compound (I guess by breeding or manipulating the genome) and culture on a medium lacking both.  They failed to mention, however, the definion of parental, so I have no idea how much genetic variation is acceptable.

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)

Edited by micro (06/16/03 10:41 PM)

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Re: Do you think this is possible ? [Re: micro]
    #1639108 -

Thanks for the research.  I found this
Quote:
Protoplasts isolated from mutant strains were fused using polyethylene glycol


somewhat disheartening.  With no more info other than "mutant strains", who knows what was really going on.

I would love to see the other reference, I guess I'll have to go to the library (30 minutes away :frown: if I want that one...

thanks


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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Re: Do you think this is possible ? [Re: mycofile]
    #1640289 -

"With no more info other than "mutant strains", who knows what was really going on."

Sorry,  I hope this helps:

ABSTRACT

Fundamental genetic analysis of the imperfect fungus Cephalosporium acremonium was undertaken following fusion between protoplasts of nutritionally complementary strains. Protoplasts were obtained in high yields from strains of C. acremonium using the commercial enzymes Cellulase CP or Novozym 234. Other factors affecting protoplast release were investigated to maximise the effectiveness of these enzymes. Optimal conditions for the reversion and fusion of protoplasts were also established.

Haploid recombinants were isolated directly from the fusion plates after plating PEG-treated protoplasts onto a variety of selective media. To minimise any adverse effects caused by the presence of an osmotic stabilizer in the medium a method was developed using cellophane discs to transfer the developing colonies onto normal media as soon as they had become resistant to osmotic shock. Using these techniques mutations at thirteen gene loci were assigned to eight different linkage groups.

The primary colonies obtained on the fusion plates were heterogeneous consisting of haploid segregants and unstable heterozygotes (aneuploids and possible diploids). Overall the results suggest that recombination in C. acremonium occurs by a similar process to that found in other fungi with a parasexual cycle.

This work has demonstrated the value of protoplast fusion methodology in genetic investigations with this fungus. Hybridization between strains of C. acremonium and Cephalosporium chrysogenum was also achieved although these are probably identical species. However, attempts to hybridize the more distantly related species, C. acremonium and Emericellopsis minima, proved unsuccessful.

Note: Cephalosporium acremonium (now known as Acremonium chrysogenum) is used commercially to produce the ?-lactam antibiotic cephalosporin C.

More results:
http://fungus.org.uk/cv/thesis_fig4.1.htm

________

A lot of other stuff can be found on the site that I have yet to read:

http://fungus.org.uk/sitemap.htm

Most of the good stuff is at the bottom :wink:

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)

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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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