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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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Re: Do you think this is possible ? [Re: Funger]
    #1610208 -

What is wrong with liquid mycelium culturing in big aerated tanks?


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Grow Mushrooms from Spores - PF-Tek for Simple Minds

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Re: Do you think this is possible ? [Re: Anno]
    #1610797 -

I'm not sure if this question was intended to be directed to me, but what the hay...

Doesn't mycelium only produce trace amounts of psilocybin/psilocin? What the author of the thread is trying to achieve is a bacteria that produces the active compounds.


Edited by Funger (06/05/03 07:22 AM)

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Re: Do you think this is possible ? [Re: ]
    #1610800 -

If you want to hammer out what is "right" or "appropriate" about the drug culture, then please do it in a different forum. This forum is for the discussion of advanced cultivation techniques and ideas, and I think that this idea definitely falls under that category.


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Re: Do you think this is possible ? [Re: Funger]
    #1610819 -

>Doesn't mycelium only produce trace amounts of psilocybin/psilocin?

Not that little in my experience.
And you can make up for the minor amounts with bigger amounts of mycelium.


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Grow Mushrooms from Spores - PF-Tek for Simple Minds

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Re: Do you think this is possible ? [Re: Anno]
    #1612065 -

Just some thoughts:
I forget who said it, but it's not bacteria but yeast that I suggested cloning into. Bacteria's sometimes tricky stuff to play with, gotta be way more sterile. Also, having smelt my fair share of bacterial cultures (cheesy shroom jars/armpits on a hot day, etc...), I"d far rather gulp down some yeast powder/ extract.
Also, like growing cannabis (were it legal) , this would take the power out of the hands of dealers/ cartels/syndicates- who ever heard of a yeast monopoly ???

"What is wrong with liquid mycelium culturing in big aerated tanks?."
Hell, if I could afford some big aerated tanks then I'd already have hired a team of scientists to bioengineer the yeasts. This is a not a shrooms vs. yeast thread I was trying to start- that's rather a lame thing to get all worked up about. Also, it's easier to hide a couple of bottles away in a dark place somewhere, and turn them over every few hours, than concealing a shitload of machinery and gleaming metallic cylinders.... "ummm.. well officer........ those are just errrr... giant fish tanks!"

Some additional side benefits ..... Psychedelic fruit wine!!! Any d.i.y shroom grower
should find this interesting if you don't already know about it:
http://www.wineworldfdw.com/fruit_wine.html
If you can already grow shrooms, this wine- making should be a breeze. However, substitute the word yeast in that article with "Psilo- yeast" (I have to give a name) and it gets a lot more interesting. Imagine sipping a glass of nice apple/ raspberry/peach wine, and tripping........ you'll have to admit it's an appealing idea.





Edited by Ghengis Khan (06/05/03 03:19 PM)

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Post deleted by Papaver [Re: Funger]
    #1612246 -


Edited by paradis (06/05/03 04:25 PM)

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Re: Do you think this is possible ? [Re: ]
    #1612317 -

Relax a little........ my sole purpose in life is not to produce "magic yeast." Other ideas that also occured to me were super enhancing human senses via: increasing the number of scent receptors in the nose, altering the cells at the back of the eyes to percieve infra red and ultra violet, installing a fluid filled cavity in the head to percieve sonar, etc..... inserting THC producing genes into common weeds...... blah blah blah, those are some I can remember. But actually going out doing these things is something different.
In the good old days I used to smoke a lot of weed, grow shrooms, and read a lot of science fiction while studying microbiology- you get some strange ideas with this combo.
But this yeast thing is entirely possible, and WILL be done in the future



Edited by Ghengis Khan (06/05/03 04:48 PM)

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Post deleted by Papaver [Re: Ghengis Khan]
    #1612359 -


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Re: Do you think this is possible ? [Re: ]
    #1612811 -

That's a really good idea :smile:

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Re: Do you think this is possible ? [Re: ]
    #1612813 -

Don't fix it if it ain't broke. What's the difference between 1 gram of shrooms and .03 grams of psilocybin? .97 grams of mushrooms, which are half the fun!

Unless your idea of fun is genetic recombination and hours upon hours of labwork (which I can understand.. :smile: ), then hey - go for it!





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The blue... the blue!!!

Edited by Rustik (06/05/03 07:41 PM)

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Re: Do you think this is possible ? [Re: Rustik]
    #1614149 -

So that's actually very inefficient Rustik, 97% of the matter is only for 50% of the fun! :wink:

But I agree, just the growing is half the fun, I wouldn't want to miss that.

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Re: Do you think this is possible ? [Re: koraks]
    #1615938 -

Well, if you don't mind losing out on the 'fun' of growing mushrooms, this is actually a rather 'simple' method to make bulk materials -- if you have access to a genetics lab and such, know which genes produce the chemicals, etc. It's used by pharmaceutical companies to produce several differnt types of drugs -- I know that most penicillin is now produced from gengineered E. Coli vats.

If you used a yeast instead of a bacteria, you could have insta-psilobycin anywhere. Packet of yeast, bucket, sugar water and some nutrients, fish tank aerator and a bubbler stone ... 12-24 hours later, you've got ten gallons of psychedelic yeast. It'd be very easy to strain the stuff and dry it. Furthermore, culturing more would be as simple as setting up another bucket and taking a few drops from the first.



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"Do we want the stars? We can have them. Can we borrow cups of fire from the sun? We can and must and light the world." --"On the Shoulders of Giants", Ray Bradbury

All of my posts are full of fiction and blatant lies.

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Post deleted by Administrator [Re: Ghengis Khan]
    #1619600 -


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Re: Do you think this is possible ? [Re: YellowPurpleHills]
    #1621871 -

I believe it would be possible if proper $ were allocated to research. But it wont be, so it wont. No home lab gonna be able to do DNA splicing, i don't think.


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Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--

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Re: Do you think this is possible ? [Re: Raadt]
    #1622648 -

Yeah, that money thing, again :wink:

--
Micro


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Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)

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Re: Do you think this is possible ? [Re: micro]
    #1625402 -

Well, yes.  Technically -- for a specialist in recombinant DNA in a fully equipped lab -- it should be pretty easy.  For you or me?  Hell no.  :wink:


--------------------
"Do we want the stars? We can have them. Can we borrow cups of fire from the sun? We can and must and light the world." --"On the Shoulders of Giants", Ray Bradbury

All of my posts are full of fiction and blatant lies.

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Re: Do you think this is possible ? [Re: Sev]
    #1625968 -

Actually, I do work with RNA in a "fully equipped lab."  They pay me, too.  :wink:

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)

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Re: Do you think this is possible ? [Re: micro]
    #1626023 -

well then, how about it?

Actually, all this talk, and we've missed the better option. Splice that gene into a Garden Giant! For those unfamiliar, garden giants fruit on a wide range of substrates from wood to straw, the beds fruit for years, and individual mushrooms weigh up to 7 pounds a piece!

So, how about it micro, are we going to see some psychoactive yeasts and garden giants coming out of your lab anytime soon?


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"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

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Re: Do you think this is possible ? [Re: mycofile]
    #1626047 -

Last time I checked the vector kit for s. cervasia (don't know how to spell that) is about $450.  Too much to come out of my pocket :wink:  We don't work with it here, so I'd need a lot of stuff we don't have.  I also don't think they'd appreciate me using their reagents for this.  It's an interesting topic of discussion, though.  Maybe some day when I have my own grant :wink:

As for other types of mushrooms, though, there has actually been some success in splicing hyphal cells' genetics between two types of fungi using polethylene glycols; this would be cheap.  Just don't know the success rate.

BTW -- I WILL do this at some point in my life, just might not be for a few years.  This is simply because it SHOULD be done.  When I do, I'm going to disseminate cultures to random people so that everyone will have them :wink:

On of my life goals.

--
Micro


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)

Edited by micro (06/11/03 09:51 AM)

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Re: Do you think this is possible ? [Re: micro]
    #1626133 -

I'M random...  :wink:

a growin original

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