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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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OfflineRustik
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Re: 22S, 23S-homobrassinolide [Re: mycofile]
    #1602654 - 06/02/03 07:54 PM (20 years, 10 months ago)

Quote:

Bee pollen is another source of homobrassino.......



Umm.. Bees make pollen?  :wink: If the bee picks the pollen up from a rapeseed, then perhaps it does. If not, then it might not. But I KNOW bees don't MAKE pollen..

lol :P 


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OfflineAnnoA
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much, much pollen [Re: ]
    #1603382 - 06/03/03 12:04 AM (20 years, 10 months ago)

>Could you just use the pollen or would it need to be extracted from the pollen?

I read that you need hundreds of kilos of rape pollen to be able to extract the amounts needed.


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Invisiblemycofile
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Re: much, much pollen [Re: Anno]
    #1603949 - 06/03/03 08:51 AM (20 years, 10 months ago)

Where did you read that anno? The reference I saw showed high concentrations of brassinosteroids in rape seed pollen. Granted, that was a while back, and I don't remember where it was, but I was always under the impression that it was one of the best organic sources.

Also, in regards to bee pollen Fungus Maximus (I think that's who) has shown definite increases in growth in liquid cultures with bee pollen. He actually even had a liquid culture form a fruit body (not from the liquid itself, but from a mass of mycelium floating on top of it). Now, like I said, that could be due to some unknown chemical, but it certainly sounds like the effects of a brassinosteroid....


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PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.

Edited by mycofile (06/03/03 08:53 AM)

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OfflineAnnoA
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Re: much, much pollen [Re: mycofile]
    #1603996 - 06/03/03 09:17 AM (20 years, 10 months ago)

>Where did you read that anno?

Believe me, a week after I read it I searched again for it for almost an hour, to no avail...

I can?t find the source anymore, but it was in the internet.


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OfflineAnnoA
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Re: 22S, 23S-homobrassinolide [Re: mycofile]
    #1604073 - 06/03/03 09:46 AM (20 years, 10 months ago)

Ok, so the motto is "never give up"

Here:
http://www.chm.bris.ac.uk/motm/brassinolide/brassinolidej.htm

"The huge effort of processing 40 kg of collected bee pollen of Brassica napus L. yielded 4 mg of a crystalline material which was identified as (22R,23R,24S)-2a,3a,22,23-tetrahydroxy- 24-methyl-B-homo-7-oxa-5a-cholestan-6-one.[3] "

More:
http://members.tripod.com/~mzullo/sourcebrassino.htm


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OfflineODhaze
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Re: 22S, 23S-homobrassinolide [Re: Anno]
    #1604406 - 06/03/03 11:47 AM (20 years, 10 months ago)



i can get a few fresh pounds of this stuff if anyone wants any of it...

i am about 95% sure that it is rape. judging from the pictures..

there is some growing next to where i work, PM me if interested, it would be worth a shot at extracting the brassinosteroids i think.


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OfflineAnnoA
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Re: 22S, 23S-homobrassinolide [Re: ODhaze]
    #1604436 - 06/03/03 11:53 AM (20 years, 10 months ago)

the pollen only, though....


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OfflineODhaze
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Re: 22S, 23S-homobrassinolide [Re: Anno]
    #1604458 - 06/03/03 12:00 PM (20 years, 10 months ago)

shit i didnt realize it was 40KG of the pollen alone,

thats a lot of work,


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I believe i will go out to the seashore, let the waves wash my mind. Gunna open up my head now just to see what i can find...

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OfflineAnnoA
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Re: 22S, 23S-homobrassinolide [Re: ODhaze]
    #1604464 - 06/03/03 12:02 PM (20 years, 10 months ago)

indeed.

still, try to get at least a few grams.


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Edited by Anno (06/03/03 12:02 PM)

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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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