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morbiddoctor
Frilly fungal fruiter



Registered: 07/05/09
Posts: 1,021
Loc: seattle, wa
Last seen: 2 years, 8 months
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Re: Pestalotiopsis microspora [Re: peatmoss]
#18033843 - 03/30/13 04:42 PM (11 years, 1 month ago) |
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Probably. There is the group buy thread.
-------------------- Trade list Updated 12/20/15 Ranco temperature controller and Wagner steamer for pasteurization in trade list! I'm looking for eyecatching, colorful, unique and interesting fungi. Not limited to edible or medicinal. Print them when you're on foray and pm me please!
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stevo

Registered: 04/11/05
Posts: 5,100
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Re: Pestalotiopsis microspora *DELETED* [Re: morbiddoctor]
#18054288 - 04/03/13 07:32 PM (11 years, 1 month ago) |
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Post deleted by stevo
Reason for deletion: .
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peatmoss
Stranger


Registered: 02/25/12
Posts: 64
Last seen: 2 years, 10 months
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Re: Pestalotiopsis microspora [Re: stevo]
#18054377 - 04/03/13 07:45 PM (11 years, 1 month ago) |
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I don't think those consist of polyurethane
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Versicolor
♖♘♗♔♕♗♘♖



Registered: 05/04/11
Posts: 2,268
Loc:
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Re: Pestalotiopsis microspora [Re: peatmoss]
#18198965 - 05/01/13 07:56 PM (11 years, 20 days ago) |
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I have been doing some research, and am a bit concerned as to whether or not it degrades polyethylene, polypropylene and other common polymers. All the studies I've seen have not said anything about using anything but polyurethane, which is only currently the 6th or 7th most commonly used polymer material. Maybe I have no idea what I'm talking about, but I would like to get some more info on this.
Also, I have read that only certain isolates of the fungus tested positive for the ability to metabolize polyurethane.
However, I could easily grow out dozens of isolates and test them all, provided I have a source for spores or a non-isolated culture. Or, I could try to fruit the ATCC culture and go from there.
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morbiddoctor
Frilly fungal fruiter



Registered: 07/05/09
Posts: 1,021
Loc: seattle, wa
Last seen: 2 years, 8 months
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Re: Pestalotiopsis microspora [Re: Versicolor]
#18201906 - 05/02/13 11:42 AM (11 years, 19 days ago) |
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Quote:
Versicolor said: I have been doing some research, and am a bit concerned as to whether or not it degrades polyethylene, polypropylene and other common polymers. All the studies I've seen have not said anything about using anything but polyurethane, which is only currently the 6th or 7th most commonly used polymer material. Maybe I have no idea what I'm talking about, but I would like to get some more info on this.
Also, I have read that only certain isolates of the fungus tested positive for the ability to metabolize polyurethane.
However, I could easily grow out dozens of isolates and test them all, provided I have a source for spores or a non-isolated culture. Or, I could try to fruit the ATCC culture and go from there.
You bring up several good points in your post. I wonder if the fungus has even been tested on other plastics. I would love to get this fungus going provided it can eat more than the above. I want to see more info on this.
-------------------- Trade list Updated 12/20/15 Ranco temperature controller and Wagner steamer for pasteurization in trade list! I'm looking for eyecatching, colorful, unique and interesting fungi. Not limited to edible or medicinal. Print them when you're on foray and pm me please!
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Rastaimposta
Apothecary


Registered: 02/21/07
Posts: 227
Last seen: 4 years, 6 months
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Has anyone actually received any of this fungus?
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greenelephant
Stranger
Registered: 10/31/12
Posts: 58
Last seen: 6 years, 6 months
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Re: Pestalotiopsis microspora [Re: Avoidit89]
#18672347 - 08/07/13 08:52 AM (10 years, 9 months ago) |
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Does anyone know which size micron filter is needed to successfully extract the fungus from growth medium? thank you
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,392
Last seen: 2 days, 17 hours
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Why would you use a filter to extract the fungus from growth medium? What are you trying to do?
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Javadog
Continuing along



Registered: 05/03/10
Posts: 7,385
Loc: USA
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Aloha does use mycelium instead of fruit bodies from which to make extractions.
How do they filter their growth media?
Interesting stuff in any case...
JD
-------------------- Boyd Rice told my brother that life is a corny pack of freesakes Myco-tek.org
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,392
Last seen: 2 days, 17 hours
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Re: Pestalotiopsis microspora [Re: Javadog]
#18705053 - 08/14/13 03:52 PM (10 years, 9 months ago) |
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My guess is that they extract from grain spawn.
I am still looking for a culture of P. microspora. I do not think it is a good solution to the plastic garbage problem because it would be far more expensive than just burying the plastic. Like all mycoremediation projects, it is highly impractical. But it would be cool to play with and send out cultures.
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,392
Last seen: 2 days, 17 hours
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Ok I finally found someone that has this culture!
She works in the Yale lab that discovered it.
You might be able to contact her and get it! She is michelle (dot) legaspi (at) yale.edu. The punctuation was spelled out to foil spam bots.
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stevo

Registered: 04/11/05
Posts: 5,100
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Post deleted by stevo
Reason for deletion: .
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WillSolvem
Odd-Hand




Registered: 04/24/12
Posts: 1,519
Loc: Chapter 26
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Re: Pestalotiopsis microspora [Re: stevo]
#19461307 - 01/23/14 07:58 AM (10 years, 3 months ago) |
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I don't want to be "that guy" but I think someone should bring it up. This species should be at least semi-regulated in the sense that it stays out of the hands of amateur mycologist. We do not fully understand this species enough to start "playing with it". I know it's a stretch for some to believe but a fungi that can exist solely on plastic alone (in anaerobic environments as well) is a danger to our technology based existence as humans.
Best case scenario we now have a fungi that degrades the plastic in our landfills.
Worst case scenario we now have a fungi that degrades the plastics in our computers, electrical infrastructure, transportation equipment, etc.
And we think trichoderma is a problem...
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AMU Q&A Thread because questions deserve answers.
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Versicolor
♖♘♗♔♕♗♘♖



Registered: 05/04/11
Posts: 2,268
Loc:
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Re: Pestalotiopsis microspora [Re: WillSolvem]
#19461489 - 01/23/14 08:48 AM (10 years, 3 months ago) |
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I can respect your opinion, but like I said above, it has only shown to biodegrade polyurethane. I doubt 'contamination' of your couch cushions with the spores of this fungus would be a problem.
-------------------- My Trade List
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WillSolvem
Odd-Hand




Registered: 04/24/12
Posts: 1,519
Loc: Chapter 26
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Re: Pestalotiopsis microspora [Re: Versicolor]
#19461520 - 01/23/14 08:55 AM (10 years, 3 months ago) |
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Right but Google a list of common urethane containing products, you will be shocked that you find MUCH more than couch cushions. CMV airbrake lines being one example that worries me.
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AMU Q&A Thread because questions deserve answers.
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,392
Last seen: 2 days, 17 hours
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Re: Pestalotiopsis microspora [Re: WillSolvem]
#19461523 - 01/23/14 08:56 AM (10 years, 3 months ago) |
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Quote:
stevo said: Thanks Alan I thought this thread had died for good. Do you think she will welcome emails from strangers though? I mean is it cool to be like: So I was reading Alans post on a magic mushroom forum... ?
Btw I dont think that works uery well spam bots anymore
I have no idea, wouldn't hurt to ask. Regarding the spam bots, I do believe they would have a very hard time turning words back into email addresses with no at sign, seems like there would be a whole lot of false positives if they tried to do that.
Quote:
WillSolvem said: I don't want to be "that guy" but I think someone should bring it up. This species should be at least semi-regulated in the sense that it stays out of the hands of amateur mycologist. We do not fully understand this species enough to start "playing with it". I know it's a stretch for some to believe but a fungi that can exist solely on plastic alone (in anaerobic environments as well) is a danger to our technology based existence as humans.
I think it's fine to play with. This species was isolated from nature and is probably not uncommon in many areas. If it was going to start eating our plastics, it would have done so a long time ago.
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WillSolvem
Odd-Hand




Registered: 04/24/12
Posts: 1,519
Loc: Chapter 26
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Re: Pestalotiopsis microspora [Re: WillSolvem]
#19461536 - 01/23/14 09:00 AM (10 years, 3 months ago) |
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AMU Q&A Thread because questions deserve answers.
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WillSolvem
Odd-Hand




Registered: 04/24/12
Posts: 1,519
Loc: Chapter 26
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Re: Pestalotiopsis microspora [Re: WillSolvem]
#19461554 - 01/23/14 09:05 AM (10 years, 3 months ago) |
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>I think it's fine to play with. This species was isolated from nature and is probably not uncommon in many areas. If it was going to start eating our plastics, it would have done so a long time ago.
Like woodlover species have exploded with the urbanisation of America plestalotiopsis my explode with the overuse of urethane products. Maybe it was just waiting for the right niche to be created and a unknowing researcher bringing it out of the amazon and into the urethane petri dish we call America.
Just a thought we should hold while dealing with this or similar species.
--------------------
AMU Q&A Thread because questions deserve answers.
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stevo

Registered: 04/11/05
Posts: 5,100
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Re: Pestalotiopsis microspora *DELETED* [Re: WillSolvem]
#19461676 - 01/23/14 09:42 AM (10 years, 3 months ago) |
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Post deleted by stevo
Reason for deletion: .
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,392
Last seen: 2 days, 17 hours
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Re: Pestalotiopsis microspora [Re: stevo]
#19461833 - 01/23/14 10:21 AM (10 years, 3 months ago) |
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Quote:
stevo said: i am still very interested I will be tuning in to see what becomes of this.
Unfortunately, nothing will probably ever become of this. I don't think it will ever be practical to break down plastic with fungi. I am not sure why anyone would want to break down plastic anyway, wouldn't it be smarter to melt it down and recycle it? If we break down plastic with mushrooms, then we have to extract more oil to make new plastic...Also we would have to burn a lot of oil to power the large autoclaves required to make enough spawn to eat a lot of plastic, the whole idea just doesn't seem very well thought out.
It would be cool if I was that guy that fucked up all the plastic in the world. I would wear that badge with honor...Unfortunately it's not going to happen. For the same reason that wood decaying fungi is ubiquitous, yet we still build things out of wood.
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