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OfflineButhoscorpio
Registered: 01/05/11
Posts: 96
Last seen: 2 years, 8 months
Re: Pestalotiopsis microspora [Re: Sharpstuff]
    #21988654 - 07/24/15 06:38 AM (8 years, 9 months ago)

I send out the cultures to european donators. They should arrive within about a week.

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OfflineKingSloth
Stranger
Registered: 03/19/15
Posts: 10
Last seen: 8 years, 5 months
Re: Pestalotiopsis microspora [Re: Avoidit89] * 1
    #22095490 - 08/15/15 04:26 PM (8 years, 9 months ago)

I got my culture and have been busy making slants, plates, and spawn-- so far so good. If successful I'll be using the spawn to start bioremediation trials!

Thanks for following through!

KS

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Offlineaktar
nerf herder

Registered: 02/27/11
Posts: 58
Last seen: 8 years, 7 months
Re: Pestalotiopsis microspora [Re: KingSloth]
    #22100250 - 08/16/15 08:03 PM (8 years, 8 months ago)

Quote:

KingSloth said:
I'll be using the spawn to start bioremediation trials!




:rockon: keep us posted! I have not had a chance to do much with it yet

does anybody know the logistics/chemistry of infusing polyeurethane into agar? if I just melt it and add it to the mix will it dissolve?

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Offlineghiajake
Myco-Viking
Male

Registered: 01/10/13
Posts: 3,897
Loc: Indiana
Last seen: 1 day, 24 minutes
Re: Pestalotiopsis microspora [Re: aktar]
    #22100373 - 08/16/15 08:40 PM (8 years, 8 months ago)

MrCloudy is working on that.

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OfflineMrcloudy
Stranger than you.
Male User Gallery

Registered: 10/01/13
Posts: 2,889
Loc: Northeast US Flag
Last seen: 8 days, 11 hours
Re: Pestalotiopsis microspora [Re: ghiajake]
    #22100429 - 08/16/15 08:59 PM (8 years, 8 months ago)

Water based polyurethane, it must be added after sterilization. If you mix it in before pressurecooking it precipitates out into strings and chunks of plastic. I was able to confirm that there does not seem to be anything in the water based polyurethane that inhibits the fungal growth, though I did confirm that my strain will not grow on straight polyurethane agar alone. 

The water based polyurethane is opaque, you can see the fungus eating it because it will clear up around the mycelium on the plate.

I will be continuing my experiments here soon, I will try sterilizing the polyurethane separately then adding it, if it can survive pressure cooking on its own.


--------------------

10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.

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Offlinemicahkid
Stranger

Registered: 07/03/12
Posts: 6
Last seen: 8 years, 8 months
Re: Pestalotiopsis microspora [Re: Mrcloudy]
    #22103019 - 08/17/15 02:36 PM (8 years, 8 months ago)

Sorry I haven't been on lately & haven't received a culture yet... I had some members involved PM me on here with address confirmation, but I haven't been on to confirm because I'm getting ready to move to NC.

Hopefully there's still a little guy waiting for me *fingers crossed* I was looking forward to checking it out.

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Offlinenomendubium
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Registered: 05/16/14
Posts: 500
Last seen: 7 years, 2 months
Re: Pestalotiopsis microspora [Re: micahkid] * 1
    #22105543 - 08/18/15 06:45 AM (8 years, 8 months ago)

got you covered


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Offlinemicahkid
Stranger

Registered: 07/03/12
Posts: 6
Last seen: 8 years, 8 months
Re: Pestalotiopsis microspora [Re: nomendubium]
    #22111679 - 08/19/15 12:59 PM (8 years, 8 months ago)

Quote:

nomendubium said:
got you covered



Thanks so much!

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Offlinemorbiddoctor
Frilly fungal fruiter
Male User Gallery


Registered: 07/05/09
Posts: 1,021
Loc: seattle, wa
Last seen: 2 years, 8 months
Re: Pestalotiopsis microspora [Re: micahkid]
    #22114544 - 08/19/15 10:31 PM (8 years, 8 months ago)

Anything I can do to get a culture?


--------------------
Trade list Updated 12/20/15

Ranco temperature controller and Wagner steamer for pasteurization in trade list!

I'm looking for eyecatching, colorful, unique and interesting fungi. Not limited to edible or medicinal. Print them when you're on foray and pm me please!

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Offlinenomendubium
 User Gallery


Registered: 05/16/14
Posts: 500
Last seen: 7 years, 2 months
Re: Pestalotiopsis microspora [Re: morbiddoctor]
    #22118231 - 08/20/15 07:27 PM (8 years, 8 months ago)

yep, you can send me $20 :pm: me


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Offlinemorbiddoctor
Frilly fungal fruiter
Male User Gallery


Registered: 07/05/09
Posts: 1,021
Loc: seattle, wa
Last seen: 2 years, 8 months
Re: Pestalotiopsis microspora [Re: nomendubium]
    #22118463 - 08/20/15 08:29 PM (8 years, 8 months ago)

^End of the month i can ☺


--------------------
Trade list Updated 12/20/15

Ranco temperature controller and Wagner steamer for pasteurization in trade list!

I'm looking for eyecatching, colorful, unique and interesting fungi. Not limited to edible or medicinal. Print them when you're on foray and pm me please!

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Offlineknomadic_niki
A mile high
 User Gallery


Registered: 06/30/14
Posts: 1,275
Loc: 6200' Colorado
Last seen: 6 years, 9 months
Re: Pestalotiopsis microspora [Re: Mrcloudy]
    #22132290 - 08/23/15 06:32 PM (8 years, 8 months ago)

Quote:

Mrcloudy said:
Water based polyurethane, it must be added after sterilization. If you mix it in before pressurecooking it precipitates out into strings and chunks of plastic. I was able to confirm that there does not seem to be anything in the water based polyurethane that inhibits the fungal growth, though I did confirm that my strain will not grow on straight polyurethane agar alone. 

The water based polyurethane is opaque, you can see the fungus eating it because it will clear up around the mycelium on the plate.

I will be continuing my experiments here soon, I will try sterilizing the polyurethane separately then adding it, if it can survive pressure cooking on its own.




awesome!


--------------------
My trade list

In search of sporeless oyster cultures

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OfflineSharpstuff


Registered: 05/04/11
Posts: 510
Loc: Australia Flag
Last seen: 7 years, 10 months
Re: Pestalotiopsis microspora [Re: knomadic_niki]
    #22150055 - 08/27/15 06:58 AM (8 years, 8 months ago)

Will also buy a culture if anyone has one,

Located in Australia !

Can you make liquid cultures of this fungi and then drop plastic in it and watch it degrade?

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Offlineseahorse
Let the hunt begin...
Male


Registered: 08/16/14
Posts: 154
Last seen: 1 year, 6 months
Re: Pestalotiopsis microspora [Re: Mrcloudy]
    #22151679 - 08/27/15 02:13 PM (8 years, 8 months ago)

This
Quote:

Mrcloudy said:
Water based polyurethane, it must be added after sterilization. If you mix it in before pressurecooking it precipitates out into strings and chunks of plastic. I was able to confirm that there does not seem to be anything in the water based polyurethane that inhibits the fungal growth, though I did confirm that my strain will not grow on straight polyurethane agar alone. 

The water based polyurethane is opaque, you can see the fungus eating it because it will clear up around the mycelium on the plate.

I will be continuing my experiments here soon, I will try sterilizing the polyurethane separately then adding it, if it can survive pressure cooking on its own.




Epic.
Can't wait for more experiments!

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OfflineSharpstuff


Registered: 05/04/11
Posts: 510
Loc: Australia Flag
Last seen: 7 years, 10 months
Re: Pestalotiopsis microspora [Re: seahorse]
    #22153705 - 08/27/15 08:39 PM (8 years, 8 months ago)

I think UV sterlization may be more ideal for sterlizing polyurethane, no?

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OfflineMrcloudy
Stranger than you.
Male User Gallery

Registered: 10/01/13
Posts: 2,889
Loc: Northeast US Flag
Last seen: 8 days, 11 hours
Re: Pestalotiopsis microspora [Re: Sharpstuff]
    #22153761 - 08/27/15 08:50 PM (8 years, 8 months ago)

Quote:

Sharpstuff said:
I think UV sterlization may be more ideal for sterlizing polyurethane, no?



I don't have a UV lamp yet.

And UV tends to only sterilize on the surface, though I do think it can be used on liquids. The water based polyurethane that I am using is opaque and would not let enough UV in to sterilize, though honestly I am not sure if the polyurethane would even be a contamination vector. Depending on the cleanliness of the factory it was made in and how careful you are about extracting it from the can, for some reason it says not to shake so I have not shook it since I opened it, in theory I should be able to pull clean polyurethane from beneath the surface, it also helps that you are only supposed to add a very small amount.

I am thinking about forgoing sterilization all together with the polyurethane and just adding it to some agar before I pour it. I just haven't had a batch of agar that wasn't already going towards another project lately.

I also need to get a hold of this culture from the co op buy. I am not sure that my personal culture has the plastic eating ability, though it could just be because I screwed my agar up the first time.


--------------------

10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.

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Offlineghiajake
Myco-Viking
Male

Registered: 01/10/13
Posts: 3,897
Loc: Indiana
Last seen: 1 day, 24 minutes
Re: Pestalotiopsis microspora [Re: Mrcloudy]
    #22155767 - 08/28/15 10:17 AM (8 years, 8 months ago)

I have the wedge Alan sent me still Mr Cloudy, you want me to send it to you?

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OfflineSharpstuff


Registered: 05/04/11
Posts: 510
Loc: Australia Flag
Last seen: 7 years, 10 months
Re: Pestalotiopsis microspora [Re: ghiajake]
    #22159097 - 08/29/15 01:13 AM (8 years, 8 months ago)

Still interested in a slant for Australia.

Will spread the love all down under!

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OfflineMrcloudy
Stranger than you.
Male User Gallery

Registered: 10/01/13
Posts: 2,889
Loc: Northeast US Flag
Last seen: 8 days, 11 hours
Re: Pestalotiopsis microspora [Re: ghiajake]
    #22159733 - 08/29/15 08:55 AM (8 years, 8 months ago)

Quote:

ghiajake said:
I have the wedge Alan sent me still Mr Cloudy, you want me to send it to you?



Thank's Ghia, my offer to send you a wedge of my strain still stands :thumbup:


--------------------

10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.

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OfflineAlan RockefellerM
Mycologist
Male User Gallery
Registered: 03/10/07
Posts: 48,392
Last seen: 2 days, 17 hours
Re: Pestalotiopsis microspora [Re: Sharpstuff]
    #22170400 - 08/31/15 06:10 PM (8 years, 8 months ago)

Quote:

Sharpstuff said:
Still interested in a slant for Australia.

Will spread the love all down under!





Slants are very hard to get into Australia due to customs restrictions.  It would be best to send a very small agar wedge in an regular letter envelope, hopefully it won't be bumpy.

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