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OfflineMrcloudy
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Re: Pestalotiopsis microspora [Re: Mrcloudy]
    #21598533 - 04/26/15 10:17 AM (9 years, 25 days ago)

http://www.sciencedirect.com/science/article/pii/S0166061614000116


Table 1, about 1/4 the way down,  Pestaliotopsis adusta the "host" was the PVC gasket of a refrigerator door. Seems other members of this genus may also have the ability to break down plastics. As mentioned above a lot of fungi probably have this ability. Seems this genus is pretty widespread, not just in the tropics, though it is more common, and the mushroom I cultured mine from is from the southern tip of Florida. 


There has been some disagreement on Mushroom observer about the culture I have. I am pretty confident it is Pestaliotopsis, wont speculate on species just yet.

Alan, I will send you some :thumbup:

Very interested to see what you come up with for testing its ability to eat plastic.

I think there could be a amateur effort to isolate strains from the wild and do tests. From my reading Pestaliotopsis is fairly common, basically a bunch of people could go out and start culturing from leaves that show symptoms of infection, or even go around swabbing old refrigerator doors or discarded styrofoam to get closer to strains with the plastic eating ability.


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10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.

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OfflineAlan RockefellerM
Mycologist
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Re: Mycology student looking for a sample. [Re: Mrcloudy]
    #21598957 - 04/26/15 12:25 PM (9 years, 25 days ago)

Quote:

Mrcloudy said:
How would one go about testing its ability to eat plastic?




Try making agar plates and put various pieces of plastic inside.

Also check the petri dish microscopically.

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InvisibleReggieJenkins
Optical Illusion
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Registered: 01/20/14
Posts: 221
Re: Mycology student looking for a sample. [Re: Alan Rockefeller]
    #21616459 - 04/30/15 06:35 AM (9 years, 21 days ago)



Things are going well on my end!

This (and every species I've tried) stuff loves modified dog food agar (9g agar, 9g dog food, 1g dextrose, 1g wood pellets) the mycelium produced is so much more dense than on PD(Y)A, I tried with this for shits and giggles, it's my new favorite agar recipe.

:mushroom2:


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I probably lost a few IQ points while using this site for a short time, it's like huffing paint but for your ego.

This site is cancer, and I will no longer encourage the spread.

You've been nothing but negative to me, and all I've tried to do is be nice.

This site is cancer.

You will never see me again.

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OfflineMrcloudy
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Re: Pestalotiopsis microspora [Re: ReggieJenkins]
    #21618966 - 04/30/15 07:24 PM (9 years, 21 days ago)

This thread and my recent finding have made me quite interested in this genus. I think that this year I will be paying close attention to grape and apple leaves that I have growing here to see about culturing some new strains.


What does everyone think? Do you think that the plastic eating is a trait shared by all strains that can be woken up with some coaxing? Or do you think it is a trait reserved to only a few strains, and thus possibly quite rare?

What would Pestalotiopsis growing on plastics in the wild even look like?


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10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.

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OfflineMrcloudy
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Re: Mycology student looking for a sample. [Re: ReggieJenkins]
    #21618974 - 04/30/15 07:27 PM (9 years, 21 days ago)

Quote:

ReggieJenkins said:


Things are going well on my end!

This (and every species I've tried) stuff loves modified dog food agar (9g agar, 9g dog food, 1g dextrose, 1g wood pellets) the mycelium produced is so much more dense than on PD(Y)A, I tried with this for shits and giggles, it's my new favorite agar recipe.

:mushroom2:





I have seen this rossette pattern a few times in the literature, I think it is pretty cool, a while back someone posted a picture of a culture they had that was doing that on facebook, I don't remember who it was, perhaps it is possible they also have a Pestalotiopsis culture. They thought it was an oyster or something like that, but I am thinking it was probably a contamination.


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10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.

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InvisibleReggieJenkins
Optical Illusion
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Posts: 221
Re: Mycology student looking for a sample. [Re: Mrcloudy]
    #21620757 - 05/01/15 06:18 AM (9 years, 20 days ago)

Do you have a sample of this species yet? I am working with the cultures today, could stick a wedge in a tube for ya and get it over.

All of the dishes I have on DFA are showing this rosette pattern, the two plates I have on PDA look like normal mycelium.

Wonder what causes this? I thought it was neat, nonetheless.

:mushroom2:


--------------------
I probably lost a few IQ points while using this site for a short time, it's like huffing paint but for your ego.

This site is cancer, and I will no longer encourage the spread.

You've been nothing but negative to me, and all I've tried to do is be nice.

This site is cancer.

You will never see me again.

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OfflineStupendous-Yappi
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Re: Pestalotiopsis microspora [Re: Mrcloudy]
    #21621641 - 05/01/15 11:19 AM (9 years, 20 days ago)

Quote:

Mrcloudy said:
What does everyone think? Do you think that the plastic eating is a trait shared by all strains that can be woken up with some coaxing? Or do you think it is a trait reserved to only a few strains, and thus possibly quite rare?



As I understand it, the rarity in that one strain is being able to live only off plastic. There are plenty of other endophyts that, to one degree or another, are able to breakdown plastic.


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OfflineMrcloudy
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Re: Pestalotiopsis microspora [Re: Stupendous-Yappi]
    #21701199 - 05/20/15 05:31 AM (9 years, 1 day ago)

My Pestalotiopsis culture recovered. I also put some of the spores onto agar and they germinated as well. I will send a wedge your way Alan, and I can send one to you as well Reggie.


--------------------

10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.

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OfflineCheebo
Earthling
Registered: 07/14/14
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Last seen: 7 years, 16 days
Re: Pestalotiopsis microspora [Re: Avoidit89]
    #21702405 - 05/20/15 01:32 PM (9 years, 1 day ago)

Hey Reggie and Allan and to whoever might be able to help me out:
I recently discovered the amazing capabilities of pestalotiopsis microspora, and simply put I want to acquire the culture or spores to cultivate and harness the plastic eating powers.

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Offlinerambo__23
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Re: Pestalotiopsis microspora [Re: Cheebo]
    #21708503 - 05/21/15 11:13 PM (8 years, 11 months ago)

yea same here.

i know its too late to donate on the indigogo campaign as it is long over, but if i donate via paypal would i be able to get a wedge, or lc?

any help would be greatly appreciated.


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OfflineAlan RockefellerM
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Re: Pestalotiopsis microspora [Re: rambo__23]
    #21710630 - 05/22/15 01:53 PM (8 years, 11 months ago)

Quote:

rambo__23 said:
yea same here.

i know its too late to donate on the indigogo campaign as it is long over, but if i donate via paypal would i be able to get a wedge, or lc?

any help would be greatly appreciated.





I'll be sending out a few next week.  People who would like one should PM me.

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OfflineMycoLab Solutions
Mycologists

Registered: 04/23/15
Posts: 5
Loc: Sebastopol, CA
Last seen: 1 year, 4 months
Re: Pestalotiopsis microspora [Re: Alan Rockefeller]
    #21720243 - 05/25/15 10:01 AM (8 years, 11 months ago)

Aloha,

I donated to the Indigogo campaign and just wanted to get an update on progress and ETA of cultures. Reggie if you could PM me that would be great.

Thanks

~ Mycolab Solutions


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MycoLab Solutions

~ Saving the world one mushroom at a time!

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Offlinepeatmoss
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Registered: 02/25/12
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Re: Pestalotiopsis microspora [Re: MycoLab Solutions]
    #21770678 - 06/06/15 02:23 PM (8 years, 11 months ago)

Would anyone be willing to send me a sample of this fungus? I'm rather eager to get a hold of it!

Does anyone think there would be interest in getting a sequencing project going for the fungus? The excess of interest shown by people wanting to acquire samples suggests that the funds for genome sequencing could easily be raised by continuing to charge for distribution of the strain.

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OfflineAlan RockefellerM
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Re: Pestalotiopsis microspora [Re: peatmoss]
    #21825396 - 06/18/15 08:33 PM (8 years, 10 months ago)

I can send out agar wedges of this - If anyone would like one, PM me an address.  If you get one, please copy the culture and send it out to others.

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Offlineleschampignons
Biochemistry + Mycology
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Re: Pestalotiopsis microspora [Re: Alan Rockefeller]
    #21830362 - 06/19/15 10:14 PM (8 years, 10 months ago)

Have the reward wedges for contributions been shipped yet? I contributed to the indiegogo but have not received a plate yet.


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OfflineStupendous-Yappi
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Re: Pestalotiopsis microspora [Re: leschampignons]
    #21830570 - 06/19/15 11:12 PM (8 years, 10 months ago)

Quote:

leschampignons said:
Have the reward wedges for contributions been shipped yet? I contributed to the indiegogo but have not received a plate yet.



:whathesaid:

What's the status ReggieJenkins?


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Offlineaktar
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Registered: 02/27/11
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Re: Pestalotiopsis microspora [Re: Stupendous-Yappi]
    #21858822 - 06/26/15 07:51 AM (8 years, 10 months ago)

I hate to suggest the worst, but it seems it's been almost 2 months since RJ has been around on the forums, and it's hard to imagine what could be causing such a delay, since the culture was delivered and reanimated quite some time ago. PLUS i am nearly certain that there are leftover funds from the IGG campaign, and I've not heard any mention of what's going to be done with those. There was originally some speak of using excess funds to look for other novel fungi with plastic-digesting potential. I would really like to see this happen, but as I understand the funds are all with Reggie. :shrug:

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Offlinemorbiddoctor
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Re: Pestalotiopsis microspora [Re: aktar]
    #21858835 - 06/26/15 07:56 AM (8 years, 10 months ago)

Would also like a wedge, should it be available.


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Trade list Updated 12/20/15

Ranco temperature controller and Wagner steamer for pasteurization in trade list!

I'm looking for eyecatching, colorful, unique and interesting fungi. Not limited to edible or medicinal. Print them when you're on foray and pm me please!

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Offlineforrest
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Re: Pestalotiopsis microspora [Re: morbiddoctor]
    #21858952 - 06/26/15 08:47 AM (8 years, 10 months ago)

About two months ago (after the group buy was made) i bought a wedge from RJ for 30$, and it also didn't arrive. But because it had to go to Europe, that could also just be because of customs. But when i pmd him about it, he also didn't respond anymore.


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OfflineAlan RockefellerM
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Re: Pestalotiopsis microspora [Re: aktar]
    #21858998 - 06/26/15 08:57 AM (8 years, 10 months ago)

Quote:

aktar said:
I hate to suggest the worst, but it seems it's been almost 2 months since RJ has been around on the forums, and it's hard to imagine what could be causing such a delay, since the culture was delivered and reanimated quite some time ago. PLUS i am nearly certain that there are leftover funds from the IGG campaign, and I've not heard any mention of what's going to be done with those. There was originally some speak of using excess funds to look for other novel fungi with plastic-digesting potential. I would really like to see this happen, but as I understand the funds are all with Reggie. :shrug:





I spoke with him on Facebook a couple days ago, and he says that he will send them but does not have the time.  I just sent another message, perhaps I can talk him into finding the time soon.

I am going to send a few out today, and that should at the very minimum get the culture out there.  I sent two wedges out last week.

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