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leschampignons
Biochemistry + Mycology



Registered: 08/30/13
Posts: 1,586
Loc: NY/NJ/ME
Last seen: 3 days, 15 hours
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Re: Mycology student looking for a sample. [Re: Rastaimposta]
#21505467 - 04/05/15 09:28 AM (9 years, 1 month ago) |
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I think people will definitely be sharing this culture. When I get mine and run it out a bit I'd certainly be happy to trade.
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,392
Last seen: 2 days, 17 hours
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Re: Mycology student looking for a sample. [Re: peatmoss]
#21520436 - 04/08/15 05:45 PM (9 years, 1 month ago) |
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Quote:
peatmoss said: What is the status of the culture? I am very much interesting in obtaining a culture. I haven't logged into this account in a couple months and so I missed the whole fundraising campaign, unfortunately!
I got a plate of it yesterday. It is really interesting looking - there are black dots all over the colonized white mycelium.
Reggie has it at his house and I think he is making the plates for everyone there. Or maybe he is giving it to someone else to copy, not sure.
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leschampignons
Biochemistry + Mycology



Registered: 08/30/13
Posts: 1,586
Loc: NY/NJ/ME
Last seen: 3 days, 15 hours
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black spots like this?

how soon do they appear? have you/will you run DNA (or perhaps just take a look under the scope) to confirm that we have the desired species?
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,392
Last seen: 2 days, 17 hours
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Re: Mycology student looking for a sample. [Re: leschampignons]
#21522136 - 04/08/15 10:32 PM (9 years, 1 month ago) |
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Yes, that is how it looked. I don't know how long the plate had run. I will scope it tomorrow, I hope and expect to see something like what you posted.
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ReggieJenkins
Optical Illusion


Registered: 01/20/14
Posts: 221
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That's how my cultures are looking as well! Black spots everywhere. Very interesting specimen here.
Rest assured everyone, I'm going balls to the walls getting plates whipped up for you all.
I've got a few master plates going, once those are at 100% in a few more days I'll pour 100 plates and transfer wedges to each.
I got a case of bubble mailers - am applying postage systematically.
Soon.
Very soon.
Alan I take it Lipa sent that on over? He was too busy to work with the culture, so received and sent it back over to me, which is the slower of the two options, but still going hot and heavy.
I've albeit abandoned this website except for this post, upon the conclusion of my sending these to everyone, I'll be logging off for good. I'm not ignoring anyone, I just haven't been on.
Reggie
-------------------- I probably lost a few IQ points while using this site for a short time, it's like huffing paint but for your ego. This site is cancer, and I will no longer encourage the spread. You've been nothing but negative to me, and all I've tried to do is be nice. This site is cancer. You will never see me again.
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ReggieJenkins
Optical Illusion


Registered: 01/20/14
Posts: 221
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Re: Mycology student looking for a sample. [Re: ReggieJenkins] 1
#21573873 - 04/20/15 10:44 PM (9 years, 30 days ago) |
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Threw together an incubator today.
Transferring wedges from master plates to 100 fresh plates tomorrow afternoon sometime. There were 80 or so donors, but I'd like to have extras so as to not be screwed if a few wedges decide not to grow.
Once all the P. microspora plates have been made, I'll work on getting the additional reward cultures (aside from the Microspora) made up and start shipping.
What - like 2 weeks or so?

Correction, I wounded myself this morning. I can't work in the SAB until my injuries are healed. Tried to amputate some fingers by accident, luckily I was not successful.
I expect I'll be able to don gloves again in a few days once I can get the bandages off of hand and get to work.
Sorry for the delay, don't play with knives when you've deprived yourself of sleep!
-------------------- I probably lost a few IQ points while using this site for a short time, it's like huffing paint but for your ego. This site is cancer, and I will no longer encourage the spread. You've been nothing but negative to me, and all I've tried to do is be nice. This site is cancer. You will never see me again.
Edited by ReggieJenkins (04/21/15 07:34 AM)
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aktar
nerf herder


Registered: 02/27/11
Posts: 58
Last seen: 8 years, 7 months
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Re: Mycology student looking for a sample. [Re: ReggieJenkins]
#21588261 - 04/23/15 10:01 PM (9 years, 27 days ago) |
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Quote:
ReggieJenkins said: I have 2 samples of the P. microspora sitting in my culture fridge

Quote:
ReggieJenkins said: Sorry for the delay, don't play with knives when you've deprived yourself of sleep!
Rest up buddy! We've been waiting months, we can hang in a few more weeks.
You have done good work keeping it all in motion  We are all indebted
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Mrcloudy
Stranger than you.


Registered: 10/01/13
Posts: 2,889
Loc: Northeast US
Last seen: 8 days, 11 hours
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Re: Mycology student looking for a sample. [Re: leschampignons]
#21588307 - 04/23/15 10:16 PM (9 years, 27 days ago) |
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Quote:
leschampignons said:
black spots like this?

how soon do they appear? have you/will you run DNA (or perhaps just take a look under the scope) to confirm that we have the desired species?
Whats up with these black spots? I have or had, I don't know if I tossed it or not yet. A random contaminant isolated from the surface of a Ganoderma from Florida. This culture produced a lot of little black areas of black liquid.
EDIT, still got it. The culture has yellowed with age. The camera flash caused a little condensation but you can see the black liquid.
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10 different Ganoderma species from across the USA AMU MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.
Edited by Mrcloudy (04/23/15 10:23 PM)
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,392
Last seen: 2 days, 17 hours
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Re: Mycology student looking for a sample. [Re: Mrcloudy]
#21588355 - 04/23/15 10:34 PM (9 years, 27 days ago) |
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I scoped the black spots in my P. microspora culture and they were 4-septate, like the above photo.
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Mrcloudy
Stranger than you.


Registered: 10/01/13
Posts: 2,889
Loc: Northeast US
Last seen: 8 days, 11 hours
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So are the black spots spore accumulations?
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10 different Ganoderma species from across the USA AMU MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,392
Last seen: 2 days, 17 hours
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Re: Mycology student looking for a sample. [Re: Mrcloudy]
#21588837 - 04/24/15 02:05 AM (9 years, 27 days ago) |
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Yes.
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Mrcloudy
Stranger than you.


Registered: 10/01/13
Posts: 2,889
Loc: Northeast US
Last seen: 8 days, 11 hours
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http://mushroomobserver.org/203284?q=2YJx9
Thanks Alan! 
I made this observation. The spore liquid had dried to a more tar like substance. The spores from mine are similar but have a more jointed appearance, perhaps because they had dried?
I might also make a post over to the Hunting and Identification forum so I don't take this thread too far off topic.
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10 different Ganoderma species from across the USA AMU MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.
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ReggieJenkins
Optical Illusion


Registered: 01/20/14
Posts: 221
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Re: Mycology student looking for a sample. [Re: Mrcloudy]
#21589153 - 04/24/15 06:35 AM (9 years, 27 days ago) |
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Back on track!
Stay tuned!
-------------------- I probably lost a few IQ points while using this site for a short time, it's like huffing paint but for your ego. This site is cancer, and I will no longer encourage the spread. You've been nothing but negative to me, and all I've tried to do is be nice. This site is cancer. You will never see me again.
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,392
Last seen: 2 days, 17 hours
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Re: Mycology student looking for a sample. [Re: Mrcloudy]
#21590108 - 04/24/15 11:07 AM (9 years, 27 days ago) |
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Quote:
Mrcloudy said: http://mushroomobserver.org/203284?q=2YJx9
Thanks Alan! 
I made this observation. The spore liquid had dried to a more tar like substance. The spores from mine are similar but have a more jointed appearance, perhaps because they had dried?
I might also make a post over to the Hunting and Identification forum so I don't take this thread too far off topic.
Wow, you have discovered a second strain. Lets evaluate that one for plastic eating ability too.
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,392
Last seen: 2 days, 17 hours
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Here are some pics of the strain I have:


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ReggieJenkins
Optical Illusion


Registered: 01/20/14
Posts: 221
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Top one was the plate I started from one of the slants I received - this stuff is pretty fast. Just tossed fresh wedges onto the ones up below the top plate today, once they have colonized enough I will pour 100 petri dishes and do a large batch of transfers and start getting things mailed out to folks.
The ones who didn't want any extra bonus culture are going out first - I've still gotta make the bonus plates for everyone who wants one. One task at a time!
Buthoscorpio your wedges went out today in the same method which I received your wedges, thanks for the reward cultures! Everyone should give Buthoscorpio 5/5 for hooking it up! International folks - I've been having trouble getting wedges through customs in tubes, you'll receive wedges in a baggie wrapped in paper in a padded envelope. I recovered all cultures from Buthoscorpio in this method, no problem.
Thanks everyone!
-------------------- I probably lost a few IQ points while using this site for a short time, it's like huffing paint but for your ego. This site is cancer, and I will no longer encourage the spread. You've been nothing but negative to me, and all I've tried to do is be nice. This site is cancer. You will never see me again.
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Mrcloudy
Stranger than you.


Registered: 10/01/13
Posts: 2,889
Loc: Northeast US
Last seen: 8 days, 11 hours
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Very cool! Thanks for the confirmation Alan! How would one go about testing its ability to eat plastic?
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10 different Ganoderma species from across the USA AMU MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.
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leschampignons
Biochemistry + Mycology



Registered: 08/30/13
Posts: 1,586
Loc: NY/NJ/ME
Last seen: 3 days, 15 hours
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Re: Mycology student looking for a sample. [Re: Mrcloudy]
#21592490 - 04/24/15 09:05 PM (9 years, 27 days ago) |
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Holy shit this thread is awesome. Why was P. microspora growing on Ganoderma? maybe eating some carbon based compounds in the conk/conk surface?
I think other strains have been observed to eat polystyrene so find some polystyrene (ps #6) and throw it in a dish with this culture maybe? Any decay would be easiest to notice if you used real thin plastic shavings I think..
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Mrcloudy
Stranger than you.


Registered: 10/01/13
Posts: 2,889
Loc: Northeast US
Last seen: 8 days, 11 hours
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Re: Mycology student looking for a sample. [Re: leschampignons]
#21592907 - 04/24/15 10:15 PM (9 years, 26 days ago) |
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Better picture of my spores, they seem to match yours Alan.
I will have to see if this is still alive since I had it sitting on a shelf instead of refrigeration. I tossed one out of the fridge a week or so to make room. (Thought it was just a boring contam). If it died I might be able to start new from spore.
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10 different Ganoderma species from across the USA AMU MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.
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Mrcloudy
Stranger than you.


Registered: 10/01/13
Posts: 2,889
Loc: Northeast US
Last seen: 8 days, 11 hours
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Re: Mycology student looking for a sample. [Re: leschampignons]
#21593012 - 04/24/15 10:40 PM (9 years, 26 days ago) |
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Quote:
leschampignons said: Holy shit this thread is awesome. Why was P. microspora growing on Ganoderma? maybe eating some carbon based compounds in the conk/conk surface?
I think other strains have been observed to eat polystyrene so find some polystyrene (ps #6) and throw it in a dish with this culture maybe? Any decay would be easiest to notice if you used real thin plastic shavings I think..
It is interesting because normally it seems to have a plant host instead of fungi. Though Ganoderma is hard and woody enough it might not make any immediate difference, or it could be environmental spores that happened to land on the Ganoderma where I scraped the material from. 
It could be a different but related mold too.
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10 different Ganoderma species from across the USA AMU MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.
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