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Offlinerichismalls
Richi-smalls

Registered: 10/19/02
Posts: 768
Loc: the ill state
Last seen: 1 day, 12 hours
Cloning
    #1598213 - 06/01/03 02:48 PM (14 years, 3 months ago)

can anyone point me to some good links on cloning. thanks :grin: 


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InvisibleEffedS
 User Gallery

Registered: 05/15/02
Posts: 7,364
Loc: Daylight Slavings
Re: Cloning [Re: richismalls]
    #1598251 - 06/01/03 03:06 PM (14 years, 3 months ago)



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Offlinerichismalls
Richi-smalls

Registered: 10/19/02
Posts: 768
Loc: the ill state
Last seen: 1 day, 12 hours
Re: Cloning [Re: Effed]
    #1608319 - 06/04/03 04:59 PM (14 years, 3 months ago)

how do i get the clone to a substrate?


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OfflineMAGICSNOOP
mob boss
Registered: 03/31/03
Posts: 394
Loc: bud park
Last seen: 14 years, 1 month
Re: Cloning [Re: richismalls]
    #1608325 - 06/04/03 05:05 PM (14 years, 3 months ago)

its in the faq cut the mushroom then take the middle or some shit the transfer to agar there a in depth explanation how to do it. pictures and all its cool


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Anonymous

Post deleted by Papaver [Re: richismalls]
    #1608528 - 06/04/03 06:06 PM (14 years, 3 months ago)



Edited by Anonymous (06/04/03 06:07 PM)


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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: Cloning [Re: ]
    #1608563 - 06/04/03 06:17 PM (14 years, 3 months ago)

Quote:

1.Build a glovebox or flowhood.
2.Make and sterilize some agar (pressure cooker required)




I've had great sucess with agar without a glovebox, flowhood or pressure cooker, they are not essential for cloning.


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PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


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Offlinerichismalls
Richi-smalls

Registered: 10/19/02
Posts: 768
Loc: the ill state
Last seen: 1 day, 12 hours
Re: Cloning [Re: Bi0TeK]
    #1608608 - 06/04/03 06:32 PM (14 years, 3 months ago)

ok so do i take the spores from the shroom that grows on the agar and thats my clone?


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Anonymous

Post deleted by Papaver [Re: Bi0TeK]
    #1608613 - 06/04/03 06:32 PM (14 years, 3 months ago)



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Anonymous

Post deleted by Papaver [Re: richismalls]
    #1608623 - 06/04/03 06:36 PM (14 years, 3 months ago)



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Offlinerichismalls
Richi-smalls

Registered: 10/19/02
Posts: 768
Loc: the ill state
Last seen: 1 day, 12 hours
Re: Cloning [Re: ]
    #1608657 - 06/04/03 06:48 PM (14 years, 3 months ago)

thank  you
:grin: 


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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: Cloning [Re: ]
    #1608755 - 06/04/03 07:18 PM (14 years, 3 months ago)

Quote:

You're correct,they are not ESSENTIAL,however it is much better to be safe and build a glovebox and PC the agar,IMO. If you're gonna do something,do it right,don't "half ass" it,thats my motto. If you've had much success with agar that wasn't PC'd I think you better thank God,because I can't see how that would work too many times.




Don't make me laugh ! I make my own peroxidated PDYA agar and have only had 2 petris turn bad out of 40 but only cos I was using a dud thermometer.

Try READING the FAQ and experiment a little, you just might learn something.  :crazy: 


--------------------
PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


Edited by Bi0TeK (06/04/03 07:19 PM)


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Anonymous

Post deleted by Papaver [Re: Bi0TeK]
    #1608868 - 06/04/03 07:58 PM (14 years, 3 months ago)



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OfflineFallenShroom
Shagadelic

Registered: 05/01/03
Posts: 879
Last seen: 9 years, 5 months
Re: Cloning [Re: ]
    #1608907 - 06/04/03 08:13 PM (14 years, 3 months ago)

paradis i don't pc my solution either with great success i guess I'm doing this half ass also open your mind just cause something is in black and white doesn't make it true you can nuke your solution or boil don't be so ignorant please


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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: Cloning [Re: ]
    #1608913 - 06/04/03 08:13 PM (14 years, 3 months ago)

Considering your a self confesed "new hand" you sure have alot of balls !  :wink: I've had a few beers etc so excuse me if I seem overly cocky but I'd be quite happy to send you a agar wedge of your choice if you like to prove my point that you don't need a autoclave/PC for agar work.

These are the only cultures on agar I have at the moment, take your pick.

P. Cubensis "Texan"
Lentinula edodes (Shiitake, Xiang Gu )
Grifola frondosa (Hen of the Woods, Maitake, kumotake)
Pleurotus ostreatus (Pearl Oyster)
Agaricus bisporus (White Button)
Cremini/Portobello (Chestnut Button)
Flammulina velutipes (winter mushroom, velvet stem, velvet foot, enoki, enokitake)

Bi0
 


--------------------
PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


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Anonymous

Post deleted by Papaver [Re: Bi0TeK]
    #1608950 - 06/04/03 08:23 PM (14 years, 3 months ago)



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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: Cloning [Re: ]
    #1608977 - 06/04/03 08:33 PM (14 years, 3 months ago)

No problem mate, all I'm saying is it works for me !  :smile:

Peroxidated agar kills bacteria and fungal spores so you can do open air transfers with little worry of contams !

Hell, I've even left open petri dishes in the dirtiest room of my house and NOTHING grew on it ! It just shriveled up as it lost moisture !  :grin:

PM me a addy and your choice of culture and I'll get it sent off to you.

Peace

Bi0 


--------------------
PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


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InvisibleChampion des Champignons
long standing member;)

Registered: 07/27/00
Posts: 2,679
Loc: Alba
Re: Cloning [Re: Bi0TeK]
    #1609894 - 06/05/03 02:00 AM (14 years, 3 months ago)

Quote:

I've had great sucess with agar without a glovebox, flowhood or pressure cooker



ditto that, as long as you use sterile petris and peroxidated agar.


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hmmm........


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