Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Bridgetown Botanicals CBD Concentrates   Left Coast Kratom Buy Kratom Capsules   Original Sensible Seeds High THC Strains   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   Myyco.com Golden Teacher Liquid Culture For Sale   PhytoExtractum Kratom Powder for Sale

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinerodent
Stranger
Registered: 12/27/02
Posts: 67
Last seen: 11 years, 7 months
Reuse Master Jar for Grain LC?
    #15928250 - 03/10/12 03:35 AM (11 years, 11 months ago)

I made a Grain LC the other day, but had a difficult time getting much mycelium from the grain-certainly plenty for enough syringes to experiment, but insufficient in the end. The master jar is about 95% regrown, and while I'd like to give it another week or so to make sure no contams set in because of excess moisture, can this same jar be used for another Grain LC extraction to the same jar I just made, because of the genetics?

Extras: Filter Print Post Top
Invisibletruskool
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: Reuse Master Jar for Grain LC? [Re: rodent]
    #15928257 - 03/10/12 03:39 AM (11 years, 11 months ago)

Yea you can do it.  Id shoot a test jar though


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

Extras: Filter Print Post Top
Offlinerodent
Stranger
Registered: 12/27/02
Posts: 67
Last seen: 11 years, 7 months
Re: Reuse Master Jar for Grain LC? [Re: truskool]
    #15928355 - 03/10/12 04:59 AM (11 years, 11 months ago)

Thinking I'll just noc up a rye jar with 2 ml of my prepped syringe and see how it does, and use it for a G2G if it looks good.

I should have shaken my master grain jar before injecting water, I think. Liberating the myc from the grain was a task, and I'm sure that's why my yield was so low. If I can use the original jar to add more myc, awesome, because it's bounced back hard in only 3 days.

Extras: Filter Print Post Top
OfflineDurban.Poison
Spore Whore
Male


Registered: 02/24/12
Posts: 269
Loc: the butthole of the USA Flag
Last seen: 11 years, 2 months
Re: Reuse Master Jar for Grain LC? [Re: rodent]
    #15928479 - 03/10/12 06:59 AM (11 years, 11 months ago)

definitely shake your master grain before adding water...make sure it is broke up really good, like you would if you were doing g2g

Extras: Filter Print Post Top
Offlinerodent
Stranger
Registered: 12/27/02
Posts: 67
Last seen: 11 years, 7 months
Re: Reuse Master Jar for Grain LC? [Re: Durban.Poison]
    #15936063 - 03/12/12 01:18 AM (11 years, 11 months ago)

Just wondering-when I PC water for a second pass on this jar, do I need an LC lid on the jar if I'm not actually going to use that jar to store the myc (putting it into the original GLC jar)? I've done my grain lids like TL's lid tek, would one of these work if I only need the water to be PC'ed, then added to the original GLC and not saved otherwise?

Edited by rodent (03/12/12 03:29 PM)

Extras: Filter Print Post Top
Offlinerodent
Stranger
Registered: 12/27/02
Posts: 67
Last seen: 11 years, 7 months
Re: Reuse Master Jar for Grain LC? [Re: rodent]
    #15938106 - 03/12/12 02:51 PM (11 years, 11 months ago)

Anyone? Anyone? Bueller?

Extras: Filter Print Post Top
Offlinerodent
Stranger
Registered: 12/27/02
Posts: 67
Last seen: 11 years, 7 months
Re: Reuse Master Jar for Grain LC? [Re: rodent]
    #15979150 - 03/22/12 12:05 AM (11 years, 11 months ago)

Sorry to dredge this up, but I'm wondering if this will cause complications:

I have 9 jars (10, but one is showing senescence) that were done via 2nd gen G2G (MS>G2G>G2G). The jar I used for the GLC (which has regrown with a vengeance...will be making a second pass extraction after getting a 60 ml syringe from a Shroomery vendor) was used for GLC after the first G2G transfer. I'd like to make two tubs with minimum 7 qts rye each. Can I combine jars of the same strain from different generations, or possibly use the rapidly colonizing test jar from the GLC for G2G with these earlier jars without issue? Might seem like a silly question, but I wonder if the myc may be weaker after so much transfer.

Extras: Filter Print Post Top
Invisibletruskool
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: Reuse Master Jar for Grain LC? [Re: rodent]
    #15979584 - 03/22/12 02:39 AM (11 years, 11 months ago)

It should be fine to use jars from any generation.  Why do you think one jar has already reached sensecence?  I highly doubt that at the second generation


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

Extras: Filter Print Post Top
Offlinerodent
Stranger
Registered: 12/27/02
Posts: 67
Last seen: 11 years, 7 months
Re: Reuse Master Jar for Grain LC? [Re: truskool]
    #15980896 - 03/22/12 11:35 AM (11 years, 11 months ago)

The jars were G2G'd on 3/2, all colonized within 8-10 days, except for one jar, which does still slowly appear to be trying, but there's a large section that still doesn't have any myc.

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Bridgetown Botanicals CBD Concentrates   Left Coast Kratom Buy Kratom Capsules   Original Sensible Seeds High THC Strains   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   Myyco.com Golden Teacher Liquid Culture For Sale   PhytoExtractum Kratom Powder for Sale


Similar ThreadsPosterViewsRepliesLast post
* Reusing Contaminated Jars? NFG_Getupkid 10,747 19 06/26/02 05:18 PM
by bassplayer74
* Reusing contaminated jars FreeCheerios 2,564 16 07/23/02 03:07 PM
by FreeCheerios
* reusing comtamed jars aenima 725 2 10/02/01 08:26 AM
by Anno
* Liquidplaya's 99% contam free grain -> X TransfTek liquidplaya 2,069 7 10/25/16 10:59 AM
by Greg
* question about grain 2 grain innoculation robinhood2 2,379 12 09/22/02 01:17 PM
by matts
* Grain Transfer from the Masters mrshrums 4,503 4 02/23/02 05:26 PM
by Suntzu
* grain grain highoman 1,083 6 03/22/02 04:00 AM
by ParticleMan
* problems with grain quarts RedHead 3,585 5 04/02/02 07:37 PM
by RedHead

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
868 topic views. 31 members, 162 guests and 29 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.008 seconds on 14 queries.