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Amazon Shop for: ½ Pint Jars, 10cc Syringe

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OfflineJohnnyRespect
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Registered: 04/17/03
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Honey Water Question
    #1597351 - 06/01/03 02:02 AM (13 years, 9 months ago)

I have a question as to honey water. I've read some posts and a few links so I'm just asking for clarity. If I were to have one syringe (10cc) filled with spore solution and 5 half pint jars with a honey-water solution (sterile) and I injected 2cc's of spore solution into each jar, would this eventually turn into a material that I could introduce onto sterile substrate and grow to mycelia?

thanks for the 411

pz


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OfflineDSD
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Re: Honey Water Question [Re: JohnnyRespect]
    #1597396 - 06/01/03 02:19 AM (13 years, 9 months ago)


Yes, that would work but in my freinds experience it's done differently. l'll post it shortly.


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OfflineDSD
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Re: Honey Water Question [Re: DSD]
    #1597416 - 06/01/03 02:28 AM (13 years, 9 months ago)


J
sorry i couldn't find it. check it out in the faq's there is some info in there. if not pm me.


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OfflineAgarico
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Re: Honey Water Question [Re: JohnnyRespect]
    #1597671 - 06/01/03 04:22 AM (13 years, 9 months ago)

Quote:

would this eventually turn into a material that I could introduce onto sterile substrate and grow to mycelia?




In that way you will make a thin spore solution with honey, this is not what it's ment to be.


You need to wait having enough mycelium in the jar, most important advantage of honeywater is to inoculate with living mycelium, this makes things go faster from the moment your solution has been colonized enough.
A few tips:
-Use very tight polifill filter
-Leave at least one third of the jar unfilled to promote breathing
-Keep jars in a clean environment
-Try to avoid contact of honeywater with  polifill filter or it could be acting  like a wick for contaminations.
-Search for long thick needles and use luer-lock syringe
-Eventually add peroxide( not before spores germination, as it will kill the spores).

Got the idea? :laugh:
   


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OfflinePDU
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Re: Honey Water Question [Re: Agarico]
    #1597753 - 06/01/03 06:12 AM (13 years, 9 months ago)

No...its not a thin spore solution, the spores germinate and grow in the honey...its just a tad slower than a tissue culture. Bandaids have worked fine as injection ports/air exchange for me with alot of success.


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OfflineAgarico
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Re: Honey Water Question [Re: PDU]
    #1598019 - 06/01/03 12:51 PM (13 years, 9 months ago)

That's what I said, thin spore solution was referred to the text I quoted :tongue: 


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Amazon Shop for: ½ Pint Jars, 10cc Syringe

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