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Offlineafflictis
ModestMycologist
Registered: 03/09/12
Posts: 334
Last seen: 5 years, 9 months
Quick question about starting a project
    #15968469 - 03/19/12 05:57 PM (11 years, 10 months ago)

Ok so someone sent me this info and i was just a little confused by how to go about this.



Quote:

Here's a process that has been 100% successful in reviving dry mushroom tissue for me so far. Don't use peroxide, whatever you do. It's toxic to fungi cells, and the last thing you want to do when reviving tissue is harm it further.

Sterilize several jars of distilled water, one of which should be prepared with small air exchange holes in the lid and a method of filtering, such as tyvek or synthetic filter disk, polyfill, etc.

Break up the dry tissue into grain sized chunks and put a dozen or so into one of your sterilized jars of water. Shake well to wash the pieces of tissue, and then transfer them to the next jar of sterile water. (you can pick up the pieces with a syringe needle or scalpel) Shake again to wash and repeat.

When you place them into the final jar, which is filtered, swirl them around in the water and then set the jar away and forget about it for a few weeks. You'll begin to see mycelium growing on any chunks that are viable. Don't use nutrients of any kind, just plain sterile distilled water. Once mycelium begins to form, transfer to agar to isolate away from any contaminants that may be present.
RR




Is it saying i can use dry mush caps and stems to get my first batch growing? Can anyone elaborate on this?


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Offlineafflictis
ModestMycologist
Registered: 03/09/12
Posts: 334
Last seen: 5 years, 9 months
Re: Quick question about starting a project [Re: afflictis]
    #15975794 - 03/21/12 10:24 AM (11 years, 10 months ago)

Bump


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