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OfflineKanji
Registered: 01/04/09
Posts: 86
Loc: Near the edge
Last seen: 6 years, 1 month
Re: Building laminar flow bood. Advice needed [Re: bakenast]
    #16392071 - 06/16/12 09:27 PM (11 years, 10 months ago)

Quote:

bakenast said:
I wonder why elaborate labortories dont just spend a few bucks on cheap walmart purifiers then instead out thousounds on flow hoods.. and if u think alcohol sterilizes everything dip ur finger in it and touch ur agar plate..




Maybe you should look at the filter efficiency and flow rates instead of wondering.

Extras: Filter Print Post Top
Offlinescienceguy
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Registered: 11/27/09
Posts: 1,983
Loc: One Mile High... Flag
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Re: Building laminar flow bood. Advice needed [Re: worowa] * 1
    #16402194 - 06/19/12 06:15 AM (11 years, 10 months ago)

Quote:

worowa said:
Growing mushies is so much easier than some folks make out. Well,some species anyway.





Like trichoderma?  Cobweb mold?  :wink:


--------------------
"Freedom starts between the ears."

Edward Abbey

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OfflineeLeSDenes
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Registered: 05/25/11
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Re: Building laminar flow bood. Advice needed [Re: bakenast]
    #16605578 - 07/28/12 11:43 AM (11 years, 9 months ago)

if you flame sterilize your hand it works :wink:

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OfflineTheUnknownPoet
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Registered: 11/14/12
Posts: 349
Last seen: 8 years, 11 months
Re: Building laminar flow bood. Advice needed [Re: bakenast]
    #17425232 - 12/20/12 11:15 PM (11 years, 4 months ago)

Quote:

bakenast said:
I wonder why elaborate labortories dont just spend a few bucks on cheap walmart purifiers then instead out thousounds on flow hoods.. and if u think alcohol sterilizes everything dip ur finger in it and touch ur agar plate..



I think you are an imbicille.

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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,091
Loc: Diving into Mystical Territori...
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Re: Building laminar flow bood. Advice needed [Re: big_herb]
    #17425458 - 12/20/12 11:59 PM (11 years, 4 months ago)

Quote:

big_herb said:
Ya know you can can just throw a 50 gallon clear garbage bag over a thirty dollar walmart air purifier and get 100% success rate. I havn't had one contam in a g2g jar in over 200 jars. costs less than 50 bucks; the flow hood thing aint worth it imo.




I do the same. It's worked for well over 200 jars, probably closer to 1,000. I just change the filter once in a while.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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Offlineskippydude
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Registered: 09/08/12
Posts: 1,827
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Re: Building laminar flow bood. Advice needed [Re: 36fuckin5]
    #17425500 - 12/21/12 12:13 AM (11 years, 4 months ago)

Quote:

36fuckin5 said:
Quote:

big_herb said:
Ya know you can can just throw a 50 gallon clear garbage bag over a thirty dollar walmart air purifier and get 100% success rate. I havn't had one contam in a g2g jar in over 200 jars. costs less than 50 bucks; the flow hood thing aint worth it imo.




I do the same. It's worked for well over 200 jars, probably closer to 1,000. I just change the filter once in a while.



I've done open air G2G in a non-sanitized room with success, but your not supposed to tell people your an idiot and I was a risk taking idiot when I did this, but that's just me:laugh2:

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OfflineeLeSDenes
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Re: Building laminar flow bood. Advice needed [Re: skippydude]
    #17426287 - 12/21/12 06:21 AM (11 years, 4 months ago)

I am really satisfied with the flowhood I built. I never get contaminations anymore.
It is easy to work with. I think that was a good investment. I can make agar in petris without worrying about contams. I could not do that in a glovebox with 100% success. So i think this is the way to go.

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Offlinescienceguy
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Re: Building laminar flow bood. Advice needed [Re: eLeSDenes]
    #17435879 - 12/23/12 07:50 AM (11 years, 4 months ago)

It comes down to how much you like to worry about whether something is going to work or not.  As the stakes get higher, and the investments larger, many people want to do what they can to minimize failures that are within their control.


--------------------
"Freedom starts between the ears."

Edward Abbey

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OfflineeLeSDenes
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Re: Building laminar flow bood. Advice needed [Re: scienceguy]
    #17435887 - 12/23/12 07:57 AM (11 years, 4 months ago)

Quote:

scienceguy said:
It comes down to how much you like to worry about whether something is going to work or not.  As the stakes get higher, and the investments larger, many people want to do what they can to minimize failures that are within their control.




Of course i like to worry about them. But what do you want to say with this? That a flowhood is totally unnecessary?

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Offlineskippydude
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Re: Building laminar flow bood. Advice needed [Re: eLeSDenes]
    #17435908 - 12/23/12 08:11 AM (11 years, 4 months ago)

I didn't realize how "necessary" a flow hood was until I got mine.

Mandatory, no.

Necessary, abso-freakin-loot-lee:thumbup:

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OfflineeLeSDenes
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Re: Building laminar flow bood. Advice needed [Re: skippydude]
    #17435917 - 12/23/12 08:15 AM (11 years, 4 months ago)

Quote:

skippydude said:
I didn't realize how "necessary" a flow hood was until I got mine.

Mandatory, no.

Necessary, abso-freakin-loot-lee:thumbup:




I can absolutely agree with you

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Offlinescienceguy
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Re: Building laminar flow bood. Advice needed [Re: eLeSDenes]
    #17435959 - 12/23/12 08:32 AM (11 years, 4 months ago)

I don't like to worry about them at all.  That's why I built a flow hood.




--------------------
"Freedom starts between the ears."

Edward Abbey

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