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Amazon Shop: ½ Pint Jars, Paul Stamets, Rye Grain

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InvisibleI_Stain_Blue
abominableshroomman

Registered: 08/19/02
Posts: 395
Loc: police state
100% colonization?
    #1596624 - 05/31/03 05:42 PM (14 years, 2 months ago)

These rye grain jars were innoculated 30 days ago with Cambodian spores.

They all appear 100% colonized:


But, then they are shaken, and they look like this:


What's going on?  Aren't they supposed to be 100% white all the way through?

They are being incubated at ~80 degrees F. :confused:


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Offlinefugu
Pooh-Bah

Registered: 01/06/03
Posts: 2,223
Loc: istanbul
Last seen: 7 years, 1 month
Re: 100% colonization? [Re: I_Stain_Blue]
    #1596636 - 05/31/03 05:49 PM (14 years, 2 months ago)

maybe gas ex.


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mushroom culture history making ...Mr. Allan is the best .....


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InvisibleI_Stain_Blue
abominableshroomman

Registered: 08/19/02
Posts: 395
Loc: police state
Re: 100% colonization? [Re: fugu]
    #1596638 - 05/31/03 05:50 PM (14 years, 2 months ago)

anyone else?


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InvisibleLiL_KuSsH
Lost In The Trip

Registered: 05/07/03
Posts: 3,001
Deleted [Re: I_Stain_Blue]
    #1596712 - 05/31/03 06:33 PM (14 years, 2 months ago)

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InvisibleI_Stain_Blue
abominableshroomman

Registered: 08/19/02
Posts: 395
Loc: police state
Re: 100% colonization? [Re: LiL_KuSsH]
    #1596746 - 05/31/03 07:10 PM (14 years, 2 months ago)

it seems more like the colonization is only occuring on the surface of the grains...so when the jar is shaken, the mycelium is pulled from the surface and the grains are exposed... I thought the mycelium is supposed to penetrate the surface of each grain and completely colonize them. ??


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InvisibleLiL_KuSsH
Lost In The Trip

Registered: 05/07/03
Posts: 3,001
Deleted [Re: I_Stain_Blue]
    #1596756 - 05/31/03 07:15 PM (14 years, 2 months ago)

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Edited by LiL_KuSsH (05/31/03 07:15 PM)


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InvisibleZwieback0
Baby Bread
 User Gallery

Registered: 08/23/02
Posts: 3,473
Re: 100% colonization? [Re: LiL_KuSsH]
    #1596776 - 05/31/03 07:32 PM (14 years, 2 months ago)

Dont dunk grain. They dont work like cakes.


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InvisibleLiL_KuSsH
Lost In The Trip

Registered: 05/07/03
Posts: 3,001
Deleted [Re: Zwieback0]
    #1596780 - 05/31/03 07:34 PM (14 years, 2 months ago)

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OfflineDSD
devil

Registered: 12/01/02
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Re: 100% colonization? [Re: Zwieback0]
    #1596786 - 05/31/03 07:37 PM (14 years, 2 months ago)


Z is right, don't dunk the grains. those pics are a little dark so it's hard to really tell. if in doubt let them sit for another week. and as said above, if the jars smell like the funk they may have contams.


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Offlinesmokey
Stranger
Registered: 05/15/03
Posts: 20
Last seen: 14 years, 13 days
Re: 100% colonization? [Re: DSD]
    #1596793 - 05/31/03 07:46 PM (14 years, 2 months ago)

That jar is ready to go, that is what happens when you shake grain. It will continue to do that as long as you shake it. I suggest using it to spawn more rye unless you already have more jars, then use it to spawn some straw/poo or just case it.


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InvisibleI_Stain_Blue
abominableshroomman

Registered: 08/19/02
Posts: 395
Loc: police state
Re: 100% colonization? [Re: smokey]
    #1596804 - 05/31/03 07:53 PM (14 years, 2 months ago)

are you serious smokey?
If it gets crumbled to be cased, there will be lots of rye surface exposed..aren't those points for contams to take hold?

because otherwise the next step is to spawn some poo


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Edited by I_Stain_Blue (05/31/03 07:56 PM)


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InvisibleWizzang
Deviant

Registered: 02/26/03
Posts: 142
Re: 100% colonization? [Re: I_Stain_Blue]
    #1596823 - 05/31/03 08:12 PM (14 years, 2 months ago)

Wait for full colonization before birthing. All other pics of completed grain jars I've seen are completely white just like pf jars. That exposed grain surface is prime territory for contams, just like if you birth a pf cake that has an area of exposed rice still, definetely not a good idea.

edit: I even looked at the first pic with a little confusion at first, it looks all light and webby. Then the second pic confirmed. Wait for that dense rhizo.


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I was once a man I used to know
He changed into a man I have been
And became the man I am today


Edited by Wizzang (05/31/03 08:15 PM)


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InvisibleLiL_KuSsH
Lost In The Trip

Registered: 05/07/03
Posts: 3,001
Deleted [Re: Wizzang]
    #1596827 - 05/31/03 08:17 PM (14 years, 2 months ago)

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InvisibleWizzang
Deviant

Registered: 02/26/03
Posts: 142
Re: 100% colonization? [Re: LiL_KuSsH]
    #1596842 - 05/31/03 08:29 PM (14 years, 2 months ago)

yea it is a long time for colonization, doesn't mean you should birth early though.


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I was once a man I used to know
He changed into a man I have been
And became the man I am today


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InvisibleLiL_KuSsH
Lost In The Trip

Registered: 05/07/03
Posts: 3,001
Deleted [Re: Wizzang]
    #1596855 - 05/31/03 08:36 PM (14 years, 2 months ago)

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InvisibleI_Stain_Blue
abominableshroomman

Registered: 08/19/02
Posts: 395
Loc: police state
Re: 100% colonization? [Re: I_Stain_Blue]
    #1596958 - 05/31/03 10:07 PM (14 years, 2 months ago)

well, look at this guy's post.

His jars look pretty much like the pics I provided. He then spawned to straw. Hmm! seems like this 100% colonization thing isn't to definite.


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Offlinejakeholman
crotch rocketsrule!

Registered: 02/24/03
Posts: 503
Last seen: 10 years, 5 months
Re: 100% colonization? [Re: I_Stain_Blue]
    #1596967 - 05/31/03 10:18 PM (14 years, 2 months ago)

stamets has before and after pics of colonized rye after shaking-its exactly what you are showing, and if you are not satisfied i can post the pics for you, but you should be good to go with that jar.
Jake H


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The above statement is fictitious and for entertainment purposes only. I do not use or condone the use of illegal substances, nor do I use or condone the use of legal substances in an illegal manner.


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Offlinemattch1
Monseur

Registered: 05/17/03
Posts: 126
Loc: WV
Last seen: 12 years, 11 months
Re: 100% colonization? [Re: I_Stain_Blue]
    #1596970 - 05/31/03 10:24 PM (14 years, 2 months ago)

Looks like a possible contamination of Bacillus. Do they smell like rotten eggs/ crotch? Ive had this problem many times before with PF cakes. To kill bacillus, you have let the jars sit for 48 hours before sterilizing/steaming. Their endospores are resistant to heat, so you have to let them sit for 2 days and let them germinate before sterilizing. If they dont have a foul odor, you might want to tip them upside down and let oxygen enter and co2 exit. Thats all i got.
Mc


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Everything I ask is for informational purposes only. There is no truth to what i say. I am lying.


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Amazon Shop: ½ Pint Jars, Paul Stamets, Rye Grain

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