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OfflineCrazyEarl
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Registered: 10/01/07
Posts: 73
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Last seen: 16 years, 1 month
agar-agar in lc?
    #7488786 - 10/05/07 03:09 PM (16 years, 5 months ago)

hi..i have a question for you all about using agar-agar in a lc...can it be used?
i bought this stuff (korean-Telephone Brand Agar Agar)for making culture dishes using the grocery store agar plate tek, but im curious if i can also use this to make lcs?

none of the lc teks ive seen that use agar mention using agar-agar in a lc its always reffeard to as just agar or other names...im wondering if anyone has used it in a lc and if it works.
anyone have a tek or recipe that they would be willing to share?
im under the impression that agar-agar is dif from the type you get from a brewing supply store. am i wrong?
any help would be great


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InvisibleCaptain Cubensis
Bleeding HeartLiberal


Registered: 09/18/07
Posts: 648
Re: agar-agar in lc? [Re: CrazyEarl]
    #7488802 - 10/05/07 03:13 PM (16 years, 5 months ago)

You use that to pour plates/petri dishes which harden/gel, not LC, which is liquid, and typically any sugar source will work at around 5%, I think malt dextrose is preferred.

Honey water

Karo water

Brown Rice syrup, although it is dark and cloudy, you can still see myc pretty well.

Or so says my foaf.


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Invisibledysphoria
lost soul

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Registered: 06/04/03
Posts: 1,651
Re: agar-agar in lc? [Re: Captain Cubensis]
    #7488805 - 10/05/07 03:15 PM (16 years, 5 months ago)

captain cubensis nailed it.
agar for gelling, simple sugars for colonization.

while agar is nutritious, it would cause problems by coalescing your liquid culture.


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OfflineCrazyEarl
Meat Popsicle
Male

Registered: 10/01/07
Posts: 73
Loc: WHAT?!
Last seen: 16 years, 1 month
Re: agar-agar in lc? [Re: Captain Cubensis]
    #7488825 - 10/05/07 03:22 PM (16 years, 5 months ago)

how well do honey lcs work on edibles such as A.bisporus? (button mushroom)
ill give it a shot and see...wont hurt to experiment


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Invisibleyutaka
Registered: 06/12/10
Posts: 544
Re: agar-agar in lc? [Re: CrazyEarl]
    #15943785 - 03/13/12 08:27 PM (12 years, 18 days ago)

Jokefox has an agar broth lc tek. I would check that out.

Depending on the agar / water ratio you could make a range of consistencies.

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