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OfflineJw6457
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Registered: 12/17/11
Posts: 434
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Inoculation question
    #15940095 - 03/12/12 09:56 PM (12 years, 10 months ago)

Hey guys, i was just wondering if my method of inoculation was not a good way because i ended up with yeast contams in all of my last set of jars and not sure what i did wrong because this method has worked great in the passed. Anyways i use tyvek filters and the red high heat silicone for injection ports. I take a piece of those wash scrubs i guess you could call it foam but anyways i soak it in alcohol cover the silicone that im going to inject through after first wilig the needle down with alcohol, then i flame sterilize the needle and stick itthroughthe alcohol soaked foam straight into the injection port and bam. Is this not a good idea. I figured if the needle is red hot it would be a good idea to stick that into the silicone because wont it melt? I was just wondering if my method is not a smart idea and if there is a better way to prdvent yeast contams or any contams please let me know thanks guys


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