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Offlinepsychoanomaly
Ἓν rὸ πᾶν
I'm a teapot


Registered: 04/22/09
Posts: 1,697
Last seen: 25 days, 11 hours
A couple quick questions before take off!
    #15931431 - 03/10/12 10:42 PM (12 years, 10 months ago)

Hey all,

I'm starting my third grow in a day or so (first grow in a few years), and there are a few things I need to clear up real quick before I get started.

I'm doing a bulk grow. For my jars I will be using 1 quart jars with WBS. I do not remember how many inoculation holes I made last time, nor how big they were. I plan to drill 4 small inoculation holes (just big enough for the spore syringe), sterilize jars with rubbing alcohol, fill 2/3 of the way with prepared WBS, cover holes with micropore tape, PC for 1.5 hours at 15 psi, and then inoculate each jar with 1? cc of spore solution in a glove box (after they cool), of course sterilizing the needle with flame and wiping with alcohol for each hole. After inoculation of jar, I will re-cover the holes with frsh micropore tape.

Questions:

1. How many cc's of solution should I use per jar? My syringes are from Ralph.

2. Is micropore tape effective for quart jars w/ WBS? Is there anything specific I should do to the tape, like sterilize it in anyway, before I use it?

3. Are four inoculation holes sufficient for each quart jar, or should I use less/more? How big should they be?

4. How much should I fill each quart jar?

5. For my pressure cooking, is 15 PSI for 1.5 hours sufficient? I live in a high altitude.


Thanks everyone! I can't wait to get started..


--------------------
Who looks outside, dreams. Who looks inside, awakes.


Are we but a moment found?
Or a moment lost, a moment unbound?

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InvisibleMollyLucyMaryJane

Registered: 09/10/11
Posts: 1,302
Re: A couple quick questions before take off! [Re: psychoanomaly]
    #15931454 - 03/10/12 10:48 PM (12 years, 10 months ago)

(1) 1.5 - 2.0 ml per half pint.
(2) Micro pore tape is fine, Masking tape will work fine as well. You don't need to sterilize it just take some from lower in the roll.
(3) Some people use 4 some use 2, I prefer 2. The hole is perfect the size of an average nail.
(4) 2/3rd of the way is fine. 3/4 is fine as well.
(5) 15 Psi for 90 Minutes is fine at any altitude.

Good luck growing!

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Offlinemrmojoshroomin
All-summa BeenNoddin
Male User Gallery


Registered: 01/24/12
Posts: 634
Loc: OH
Last seen: 6 years, 7 months
Re: A couple quick questions before take off! [Re: MollyLucyMaryJane]
    #15931735 - 03/10/12 11:48 PM (12 years, 10 months ago)

Make sure u shake your jars after u PC them!!!!

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Offlinepsychoanomaly
Ἓν rὸ πᾶν
I'm a teapot


Registered: 04/22/09
Posts: 1,697
Last seen: 25 days, 11 hours
Re: A couple quick questions before take off! [Re: mrmojoshroomin]
    #15932024 - 03/11/12 01:41 AM (12 years, 10 months ago)

Thanks guys! Gonna start soaking the WBS tomorrow!

Wish me luck!


--------------------
Who looks outside, dreams. Who looks inside, awakes.


Are we but a moment found?
Or a moment lost, a moment unbound?

Extras: Filter Print Post Top
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