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Invisiblehelix
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Registered: 09/13/10
Posts: 409
Re: how many times do you shake rye grain jars before they're considered "fully colonized"? [Re: rohr]
    #15929368 - 03/10/12 01:18 PM (12 years, 10 months ago)

Quote:

rohr said:
Quote:

aronf13 said:
My jars all looked like the one on the left but when I shake them up against a phone book they look like the one on the right. Do I have to keep incubating them or is it that once it has looked like the one on the left at least once, it's good to go into the tub?





a fully colonized jar will appear almost completely void of mycelium after it's shaken. it's ok, the mycelium will recover! no need to wait any longer, pour the broken up rye into your tub (or tray or whatever) after shaking.





yay. thank you!

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OfflineChromeCrow
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Re: how many times do you shake rye grain jars before they're considered "fully colonized"? [Re: Shamrocker]
    #15929530 - 03/10/12 02:02 PM (12 years, 10 months ago)

Quote:

Shamrocker said:
If you're using a MS syringe, don't shake. You can shake it if you are using LC.




each tiny drop of MS spore solution  has hundreds or thousands of spores. There is NO way you gonna separate them by shaking.
you WANT to distribute the spore water around the grain, so it will colonize through out the jar, not just at the points the spore solution trickles down


--------------------
ISO: Orissa, Malabar, z strain

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OfflineShamrocker
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Re: how many times do you shake rye grain jars before they're considered "fully colonized"? [Re: ChromeCrow]
    #15929954 - 03/10/12 04:03 PM (12 years, 10 months ago)

If you're going to shake the jar at 25% or so colonization anyway to distribute the myc, maybe it helps that you have a clump of myc instead of a bunch of isolated growth spots.
Honestly, I don't know for certain. All I can say is it works for me.

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Offlinemrmojoshroomin
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Re: how many times do you shake rye grain jars before they're considered "fully colonized"? [Re: ChromeCrow]
    #15930577 - 03/10/12 07:00 PM (12 years, 10 months ago)

Quote:

ChromeCrow said:
Quote:

Shamrocker said:
If you're using a MS syringe, don't shake. You can shake it if you are using LC.




each tiny drop of MS spore solution  has hundreds or thousands of spores. There is NO way you gonna separate them by shaking.
you WANT to distribute the spore water around the grain, so it will colonize through out the jar, not just at the points the spore solution trickles down



ChromeCrow I think u and everyone who agree's with you are wrong...This is straight outta RR's notebook>>>> *Shaking right after inoculation is a mistake. You should allow the spores to germinate in close proximity to each other so they can pair up and become dikaryotic. Monokaryotic mycelium is thinner and much slower growing than dikaryotic mycelium. By shaking the jar, you required it to colonize with the slower growing monokaryons. SHAKING GRAIN JARS

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Invisiblecubes4cancer
C0nn0IssuR Of s0rtS


Registered: 01/11/12
Posts: 1,527
Loc: thiswashereandthatwaswhen
Re: how many times do you shake rye grain jars before they're considered "fully colonized"? [Re: mrmojoshroomin]
    #15930647 - 03/10/12 07:12 PM (12 years, 10 months ago)

copy and paste much??lol jk :crazy2:
RRs notes are one of the few things i bookmark,and for good reason :grin::thumbup:


--------------------
$$"IM ON A RAMAN NOODLE EVERY NIGHT BUDGET"$$
                                        :ramen:



ROGERRABBIT: P cubensis will grow on stuffed animals, bibles, quarter pounds of weed, bras, etc.
In other words,it's hard to fuck up a cube grow.
RR

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Offlinemrmojoshroomin
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Re: how many times do you shake rye grain jars before they're considered "fully colonized"? [Re: cubes4cancer]
    #15930661 - 03/10/12 07:16 PM (12 years, 10 months ago)

Quote:

cubes4cancer said:
copy and paste much??lol jk :crazy2:
RRs notes are one of the few things i bookmark,and for good reason :grin::thumbup:



lol, soo we gotta wise guy, nyuk nyuk nyuk, yeah his and Agar's are about all the research ANYbody needs for GENERAL cultivation

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Invisiblecubes4cancer
C0nn0IssuR Of s0rtS


Registered: 01/11/12
Posts: 1,527
Loc: thiswashereandthatwaswhen
Re: how many times do you shake rye grain jars before they're considered "fully colonized"? [Re: mrmojoshroomin]
    #15930675 - 03/10/12 07:19 PM (12 years, 10 months ago)

i concur 100% :smile:


--------------------
$$"IM ON A RAMAN NOODLE EVERY NIGHT BUDGET"$$
                                        :ramen:



ROGERRABBIT: P cubensis will grow on stuffed animals, bibles, quarter pounds of weed, bras, etc.
In other words,it's hard to fuck up a cube grow.
RR

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