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Invisiblebait_
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no growth on grain after 1 week after inoc with an lc...
    #15930240 - 03/10/12 05:26 PM (12 years, 10 months ago)

would you be worried?

i'm talking like no visible growth and i inoculated heavy (~6ccs)

thoughts/comments?

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OfflineShamrocker
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Re: no growth on grain after 1 week after inoc with an lc... [Re: bait_]
    #15930284 - 03/10/12 05:36 PM (12 years, 10 months ago)

Did you test your LC before noccing up the grain?  Or are these the test jars?

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Invisiblebait_
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Re: no growth on grain after 1 week after inoc with an lc... [Re: Shamrocker]
    #15930305 - 03/10/12 05:41 PM (12 years, 10 months ago)

lc is viable

~3 good grows previous to it using same lc

no sign of bacteria but no growth

i feel i should at least be seeing little with balls on the top of the grain or growth down the walls

used fooman's tek (same tek as previous grows) -- the only thing i did differently was not rinse WBS before 'boiling'

pc for 2 full hours and let cool naturally

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OfflineShamrocker
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Re: no growth on grain after 1 week after inoc with an lc... [Re: bait_]
    #15930336 - 03/10/12 05:47 PM (12 years, 10 months ago)

That is odd. How long ago was the LC created? Fridge stored I'm assuming?

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Invisiblecubes4cancer
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Re: no growth on grain after 1 week after inoc with an lc... [Re: Shamrocker]
    #15930342 - 03/10/12 05:51 PM (12 years, 10 months ago)

it will show,give it another week then worry :thumbup:


--------------------
$$"IM ON A RAMAN NOODLE EVERY NIGHT BUDGET"$$
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ROGERRABBIT: P cubensis will grow on stuffed animals, bibles, quarter pounds of weed, bras, etc.
In other words,it's hard to fuck up a cube grow.
RR

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Invisiblebait_
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Re: no growth on grain after 1 week after inoc with an lc... [Re: cubes4cancer]
    #15930352 - 03/10/12 05:55 PM (12 years, 10 months ago)

lc created 10/11

not fridge stored but stored in a 'secluded' room with no heat vents during a michigan winter.

lc was viable about 6weeks ago

probably ~50°Favg

i think i will give it another week or so.  i'm just worried because it's been a week and out of 10 jars i'm not seeing any growth.  this is the 1st time i checked them (¡¿maybe they've been slow due to lack of good vibes?!)

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OfflineShamrocker
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Re: no growth on grain after 1 week after inoc with an lc... [Re: bait_]
    #15930363 - 03/10/12 05:58 PM (12 years, 10 months ago)

Haha maybe! Don't give up on them yet. In the meantime, you might want to whip up a new batch of LC.

Good Luck, man!

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InvisibleViolet
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Re: no growth on grain after 1 week after inoc with an lc... [Re: Shamrocker]
    #15930368 - 03/10/12 06:00 PM (12 years, 10 months ago)

I find this has many variables. I took 2 biopsy clones of a recent grow, 1 large invitro pin and 1 large fruit quickly up. The invitro pin clone has taken a week and a half to show just the SLIGHTEST bit of growth, over seven jars. The large fruit clone culture was inoculated just two days ago and is already far ahead.
:shrug:
Both cultures grew identically - lovely large mycelial perfect orbs before being broken up - and both in Karo LCs.

I thought the delay may have been from using a culture that was very used to simple sugars, and in the case of my clone only having started on BRF. But frankly it just seems that it will grow when it's ready.


Main thing to tell you is about mine not showing fuzz for a week and half.


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Invisiblebait_
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Re: no growth on grain after 1 week after inoc with an lc... [Re: Shamrocker]
    #15930396 - 03/10/12 06:10 PM (12 years, 10 months ago)

Quote:

Shamrocker said:
Haha maybe! Don't give up on them yet. In the meantime, you might want to whip up a new batch of LC.

Good Luck, man!




got an unused lc made the same time (looks clean and should be because i used the same S-syringe).
even when an lc is fully colonized i am worried about cross contamination.

i am going to give it another ~week. next time i'll be able to spend time on this hobby.

also LC is LME

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OfflineShamrocker
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Re: no growth on grain after 1 week after inoc with an lc... [Re: bait_]
    #15930428 - 03/10/12 06:19 PM (12 years, 10 months ago)

Cool! I use ELME ala Transcending Life's tek. They are usually pretty fast colonizing .... but not always! Lol

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Offlinethedoc8
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Re: no growth on grain after 1 week after inoc with an lc... [Re: bait_]
    #15932520 - 03/11/12 09:30 AM (12 years, 10 months ago)

Quote:

bait_ said:
would you be worried?

i'm talking like no visible growth and i inoculated heavy (~6ccs)

thoughts/comments?



No one ask what temp are your at.

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