Home | Community | Message Board

Mycohaus
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: MagicBag.co All-In-One Bags That Don't Suck   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds High THC Strains   PhytoExtractum Kratom Powder for Sale

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineafflictis
ModestMycologist
Registered: 03/09/12
Posts: 334
Last seen: 6 years, 9 months
New to growing need advice on agar
    #15924113 - 03/09/12 11:03 AM (12 years, 10 months ago)

Hey guys, just started up a little growing project. I got some agar plates and took some dry mushroom samples to see if i could get some spores from them, however i have no idea what im really looking for as far as mold/mycelium sheets. Any advice would be greatly appreciated!


Extras: Filter Print Post Top
Invisiblepsychotropical

Registered: 10/15/11
Posts: 235
Re: New to growing need advice on agar [Re: afflictis]
    #15924390 - 03/09/12 12:35 PM (12 years, 10 months ago)

Hmm, it's tough to tell from the fuzzy pics - it kind of looks like a mess.

You can also use the tissue from the shroom if you rehydrate it.

You're going to be battling contams though since the spores & shroom tissue aren't sterile -  so you need to work with small samples and transfer good growth on the leading edge of the myc away from the contams until you isolate it.

It's all about methodical sterile procedure & isolation. What you're looking for is agressive growth of rhizomorphic (stringy/thready - not tomentose/cottony) white mycelium. Check out RR's video "Mushroom Strain Isolation"

Good luck :thumbup:

Edited by psychotropical (03/09/12 12:43 PM)

Extras: Filter Print Post Top
Offlinesgfcchamber
Stranger
Registered: 10/15/11
Posts: 554
Last seen: 8 years, 8 months
Re: New to growing need advice on agar [Re: psychotropical]
    #15924789 - 03/09/12 02:01 PM (12 years, 10 months ago)

You mentioned you took dry mushroom samples.  Here is a quote from RR from this thread.

Quote:

Here's a process that has been 100% successful in reviving dry mushroom tissue for me so far. Don't use peroxide, whatever you do. It's toxic to fungi cells, and the last thing you want to do when reviving tissue is harm it further.

Sterilize several jars of distilled water, one of which should be prepared with small air exchange holes in the lid and a method of filtering, such as tyvek or synthetic filter disk, polyfill, etc.

Break up the dry tissue into grain sized chunks and put a dozen or so into one of your sterilized jars of water. Shake well to wash the pieces of tissue, and then transfer them to the next jar of sterile water. (you can pick up the pieces with a syringe needle or scalpel) Shake again to wash and repeat.

When you place them into the final jar, which is filtered, swirl them around in the water and then set the jar away and forget about it for a few weeks. You'll begin to see mycelium growing on any chunks that are viable. Don't use nutrients of any kind, just plain sterile distilled water. Once mycelium begins to form, transfer to agar to isolate away from any contaminants that may be present.
RR




--------------------

========================================================================================
Mushroom / Bulk Substrate Calculator <- Tells you exactly how much of what ingredients you need for any substrate for any container!
Here is the corresponding shroomery thread: http://www.shroomery.org/forums/showflat.php/Number/15656577


Easy Bulk Substrate Pasteurizer - Hands Free Operation
========================================================================================

Extras: Filter Print Post Top
Invisiblepsychotropical

Registered: 10/15/11
Posts: 235
Re: New to growing need advice on agar [Re: sgfcchamber]
    #15924816 - 03/09/12 02:07 PM (12 years, 10 months ago)

:whathesaid:  there you go  :billymaythumbup:

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: MagicBag.co All-In-One Bags That Don't Suck   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds High THC Strains   PhytoExtractum Kratom Powder for Sale


Similar ThreadsPosterViewsRepliesLast post
* Why grow on agar? stcore 2,057 1 07/29/02 05:03 PM
by bluhoney
* Innoculation with agar wedge Visigoth 5,665 6 01/15/23 12:59 PM
by Screwup
* . *DELETED*
( 1 2 all )
Fd3000 5,914 22 08/22/02 11:08 PM
by Zen Peddler
* Re: Growing shrooms on a bioreactor paddoholland 2,115 13 03/20/01 11:45 PM
by paddoholland
* Re: Use agar and break free from spore vendor dependency forever for only $10!
( 1 2 3 all )
Anonymous 7,723 41 02/04/01 12:43 PM
by puscle
* Pastywhyte's Easy Agar Tek
( 1 2 3 4 ... 376 377 )
PastywhyteMDiscord 820,245 7,536 12/09/24 09:50 AM
by InnerWisdom
* Benefits of Agar? celsius 9,660 10 12/01/19 12:36 AM
by wildernessjunkie
* Re: mold in agar plate Anonymous 13,428 3 12/04/99 12:35 AM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
236 topic views. 20 members, 93 guests and 24 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.018 seconds spending 0.007 seconds on 12 queries.