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afflictis
ModestMycologist
Registered: 03/09/12
Posts: 334
Last seen: 6 years, 9 months
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New to growing need advice on agar
#15924113 - 03/09/12 11:03 AM (12 years, 10 months ago) |
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Hey guys, just started up a little growing project. I got some agar plates and took some dry mushroom samples to see if i could get some spores from them, however i have no idea what im really looking for as far as mold/mycelium sheets. Any advice would be greatly appreciated!
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psychotropical

Registered: 10/15/11
Posts: 235
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Re: New to growing need advice on agar [Re: afflictis]
#15924390 - 03/09/12 12:35 PM (12 years, 10 months ago) |
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Hmm, it's tough to tell from the fuzzy pics - it kind of looks like a mess.
You can also use the tissue from the shroom if you rehydrate it.
You're going to be battling contams though since the spores & shroom tissue aren't sterile - so you need to work with small samples and transfer good growth on the leading edge of the myc away from the contams until you isolate it.
It's all about methodical sterile procedure & isolation. What you're looking for is agressive growth of rhizomorphic (stringy/thready - not tomentose/cottony) white mycelium. Check out RR's video "Mushroom Strain Isolation"
Good luck
Edited by psychotropical (03/09/12 12:43 PM)
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sgfcchamber
Stranger
Registered: 10/15/11
Posts: 554
Last seen: 8 years, 8 months
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Re: New to growing need advice on agar [Re: psychotropical]
#15924789 - 03/09/12 02:01 PM (12 years, 10 months ago) |
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You mentioned you took dry mushroom samples. Here is a quote from RR from this thread.
Quote:
Here's a process that has been 100% successful in reviving dry mushroom tissue for me so far. Don't use peroxide, whatever you do. It's toxic to fungi cells, and the last thing you want to do when reviving tissue is harm it further.
Sterilize several jars of distilled water, one of which should be prepared with small air exchange holes in the lid and a method of filtering, such as tyvek or synthetic filter disk, polyfill, etc.
Break up the dry tissue into grain sized chunks and put a dozen or so into one of your sterilized jars of water. Shake well to wash the pieces of tissue, and then transfer them to the next jar of sterile water. (you can pick up the pieces with a syringe needle or scalpel) Shake again to wash and repeat.
When you place them into the final jar, which is filtered, swirl them around in the water and then set the jar away and forget about it for a few weeks. You'll begin to see mycelium growing on any chunks that are viable. Don't use nutrients of any kind, just plain sterile distilled water. Once mycelium begins to form, transfer to agar to isolate away from any contaminants that may be present. RR
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Mushroom / Bulk Substrate Calculator <- Tells you exactly how much of what ingredients you need for any substrate for any container!
Here is the corresponding shroomery thread: http://www.shroomery.org/forums/showflat.php/Number/15656577
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psychotropical

Registered: 10/15/11
Posts: 235
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Re: New to growing need advice on agar [Re: sgfcchamber]
#15924816 - 03/09/12 02:07 PM (12 years, 10 months ago) |
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there you go
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