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Offlinesgfcchamber
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Registered: 10/15/11
Posts: 554
Last seen: 8 years, 8 months
Agar Wedge Placed in Grain Jar Ended Up Smushed Against Glass...Problem?
    #15915716 - 03/07/12 07:08 PM (12 years, 10 months ago)

Took a nearly fully colonized plate and split it into two agar wedges.

One wedge was placed in one jar and the other wedge in another.

A small shake was given just to get the wedge covered up a little bit by the grains, which was achieved, but in the process the wedge turned sideways and the side that was in contact with the bottom of the petri is now smushed up against the glass...totally stuck to it.  This happened in both jars.

Is this a problem that will lead to a breeding ground for contams?


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Offlinesteelmonkey
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Registered: 02/22/11
Posts: 1,822
Loc: Nova Scotia,Canada Flag
Last seen: 3 years, 3 months
Re: Agar Wedge Placed in Grain Jar Ended Up Smushed Against Glass...Problem? [Re: sgfcchamber]
    #15915837 - 03/07/12 07:26 PM (12 years, 10 months ago)

It shouldn't be a problem as long as the wedge has contact with the grains

In the future try tilting the jar and letting the grains slide to a angle then place the agar wedge and stand the jar upright letting the grains fall against the wedge,let the mycelium grow out a bit then shake


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InvisibleMr.PhilCybin
Master Baiter
I'm a teapot

Registered: 06/13/11
Posts: 11,646
Loc: Gnarnia
Re: Agar Wedge Placed in Grain Jar Ended Up Smushed Against Glass...Problem? [Re: steelmonkey]
    #15915878 - 03/07/12 07:34 PM (12 years, 10 months ago)

:whathesaid:

This happened to a couple of my jars as well.
I just shook the shit out of it to get the grains to "scrape" off some myc onto them and it was fine.  A shake after about a week and everything was moving right along. :thumbup:


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