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InvisiblePorciniMan
Mustard Tiger


Registered: 05/29/08
Posts: 58
Colonization notes on 5 different strains.
    #15913891 - 03/07/12 12:56 PM (12 years, 10 months ago)

I am currently incubating 12 jars of 5 different strains on WBS. Currently I am "cold incubating" meaning I am using no heat source to incubate them yet. I'm not sure if this is a commonly used term I just made it up. They are in my closet between 50-60 degrees so they are colonizing quite slowly. The reason for this is because I am waiting to see if I get a job out of town that pays well and I will not be able to take them with me in which case I will have to find someone to adopt them. This may require me to train someone real quick before I leave in early april. If I end up staying, I will apply heat and speed the proccess and continue with the grow.

Anyway, the progress of the colonization of these "cold incubated" jars are as follows. Note that at day 2 these jars where transported 300 miles because I moved.

The R44 and Acadian Coast strains showed signs of colonization at exactly 7 days.
The Mexi strain showed signs of colonization at 8 days.
The Panama strain has one single grain starting to colonize at 9 days.
The Puerto Rico strain still has no colonization at day 10 with the R44 and Acadian strains going strong and the Mexis catching up.

These results were nowhere close to what I expected at innooculation. I was expecting the Mexis to colonize first because it looked more like myceliated water than spores and the Puerto Rico syringe I used had a very high spore count. I'm curious if the real results are due to genetics. Panama and Puerto Rico being warmer climates, I assume they don't do as well in the cold temps as other strains.

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OfflineKizzle
Misanthrope
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Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 7 days
Trusted Cultivator
Re: Colonization notes on 5 different strains. [Re: PorciniMan]
    #15913980 - 03/07/12 01:18 PM (12 years, 10 months ago)

The random genetics that are formed during a multispore grow are going to be more significant than the supposed traits of the various variety of cubensis sold by vendors. I always take the descriptions given with a grain of salt even though it is fun to try new strains (they actually don't fit the scientific definition of a strain so I try to use 'variety')


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OfflineDr.Tooty
Eye see you.
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Registered: 06/03/11
Posts: 2,003
Loc: Nowhere in particular. Flag
Last seen: 8 years, 3 months
Re: Colonization notes on 5 different strains. [Re: PorciniMan]
    #15913986 - 03/07/12 01:20 PM (12 years, 10 months ago)

Quote:

PorciniMan said:
I'm curious if the real results are due to genetics. Panama and Puerto Rico being warmer climates, I assume they don't do as well in the cold temps as other strains.




All P. cubensis. All spores came from fruits not in puerto rico or panama...so :shrug:

It's just called the colonization period/stage/whatever. not "cold incubation".
and only people in very cold places need to incubate.


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"I get up, I get down." Insect Forum

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