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ULTIMAH
Stranger
Registered: 02/17/10
Posts: 185
Last seen: 8 years, 4 months
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How can i use bags instead of jars to colonize wbs?
#15912485 - 03/07/12 03:23 AM (12 years, 10 months ago) |
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I have used long time jars to colonize wbs, but now i want to make it a little easier to make bigger amounts of colonized wbs with bags but i have no clue how to make a bag that i can put to pressure cooker.
Can somebody give me some advices how its done?
I read somewhere that if you use bags, then you have to inoculate it with liquid culture, is that true or can i mix it with already colonized wbs?
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joshisstoned
Motorcycle Enthusiast


Registered: 05/24/09
Posts: 3,544
Loc: Ohio
Last seen: 10 years, 5 months
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Re: How can i use bags instead of jars to colonize wbs? [Re: ULTIMAH]
#15912557 - 03/07/12 04:21 AM (12 years, 10 months ago) |
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I just prepped the seed, and wrapped the bag tight with rubber bands and rolled it in foil like a huge sub sandwich. Set it on empty jars on their side to keep out of water. When done and cool unwrap and tie the top with a rubber band. Undo rubber band and add agar wedge/wbs spawn, etc. Worked for me but I'm lucky.......miss the old pre-kid days.
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punkrocker292004
i am a liar



Registered: 12/17/09
Posts: 2,921
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Re: How can i use bags instead of jars to colonize wbs? [Re: joshisstoned]
#15912613 - 03/07/12 04:50 AM (12 years, 10 months ago) |
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-------------------- EVERYTHING YOU NEED TO KNOW
on a long enough timeline the survival rate for everyone drops to zero-tyler durden
watch me
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ChromeCrow
one ancient mutha




Registered: 02/21/02
Posts: 1,887
Loc: Hoosier HELL
Last seen: 1 year, 9 months
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Re: How can i use bags instead of jars to colonize wbs? [Re: ULTIMAH]
#15912865 - 03/07/12 07:15 AM (12 years, 10 months ago) |
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Quote:
ULTIMAH said:
I read somewhere that if you use bags, then you have to inoculate it with liquid culture, is that true or can i mix it with already colonized wbs?
NO I use bags for grain spawn, and substrate. you can use MS, LC, G2G, agar wedges etc.. ANYTHING you use jars for.
I also sterilize my substrate ( No flames) in bags.
If you have an impulse sealer, then it is really easy, just make sure you use presealable bags. If not thats ok, you can go "old school"
*Place grain/substrate etc in bag *cut a syringe in 1/2, with open ends on both sides. *feed the bag end through the syringe, and bag down ( end will now be on the oustide of the syringe piece)

*fasten with rubber band

*fill the open end with polifill

*I like to add a doubled coffee filter over the polifill, use another rubber band to hold the coffee filter on.

*remove as much air as possible from the bag. *place foil over the end to keep water from getting into the bag.

*fold the extra bag accordian style. *place in you pc, BUT make sure no part of the bag touches the metal ( sides,bottom of the pc ) I use a large pre-wetted towel to esnure the bags dont touch any metal
IMPORTANT, you want to weight down the bags in some way, either a plate, an old weight, or as i am now using a brick.. you want something to make sure that if the bag expands, it doesn't block the pressure release valve or you could have an explosion
-------------------- ISO: Orissa, Malabar, z strain
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crimsondrac
Stranger
Registered: 12/09/11
Posts: 493
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Re: How can i use bags instead of jars to colonize wbs? [Re: ChromeCrow]
#15913086 - 03/07/12 08:59 AM (12 years, 10 months ago) |
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I have heard you can use the heavy duty ziplock freezer bags in pressure cookers. Just wrap in foil like the other poster said. I also use the ziplock or Glad brand Plastic containers. Any container really that is Recycle-5. They come in all sizes from small to large and are pretty easy to work with.
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ubimarble
Stranger than most!



Registered: 01/14/11
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Last seen: 7 years, 5 months
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Re: How can i use bags instead of jars to colonize wbs? [Re: punkrocker292004]
#15913119 - 03/07/12 09:15 AM (12 years, 10 months ago) |
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Quote:
punkrocker292004 said:

-------------------- I wouldn't trust a thing I say, most of it is made up anyway.
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