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OfflineTheFreeman
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Registered: 12/07/11
Posts: 40
Last seen: 12 years, 2 months
MS syringe to petri dish contam
    #15910974 - 03/06/12 07:55 PM (12 years, 10 months ago)

I prepped 12 dishes, I inoculated 3 with a spore syringe, left one sitting out as a control, and stuck the rest in the fridge. It's my first attempt at agar work (with a glove box). The three have become colonized with nasty pink stuff, but the control is clean. Could this mean the syringe is contaminated? I did use it once prior to this. And if my sterile procedure wasn't good enough, would I be seeing visible contams on the control dish?

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Offlinebilly


Registered: 07/13/11
Posts: 554
Last seen: 6 years, 10 months
Re: MS syringe to petri dish contam [Re: TheFreeman]
    #15911135 - 03/06/12 08:22 PM (12 years, 10 months ago)

its easy to have a contam from ms prints are not 100% sterile.  or your control didnt get a contam  because it was never opened...sterilize yourself, materials and the glove box better?

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InvisibleDoc_T
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Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: MS syringe to petri dish contam [Re: TheFreeman]
    #15911143 - 03/06/12 08:23 PM (12 years, 10 months ago)

Control dish was clean, but all three you used the syringe on showed the same contam?
High five dude, you've proven your syringe is bad a couple months ahead of the typical grower.
:highfive:

If there's mycelium, you may be able to transfer some to a clean dish and salvage.

(One control dish isn't 'proof', but it's good enough for me.)


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Offlineb4n4n4r4m4
bananas!


Registered: 01/24/12
Posts: 284
Last seen: 1 year, 2 months
Re: MS syringe to petri dish contam [Re: billy]
    #15911153 - 03/06/12 08:25 PM (12 years, 10 months ago)

More than likely it was something you did. If you bought your spores from a trusted vendor most lkely its not their fault. They have good reviews and are "trusted" for a reason. I think the way to do agar in the future is to make/sterilize them yourself like billy said, and Go from LC or take a clone. (Or your own spore print maybe?)


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OfflineTheFreeman
Male

Registered: 12/07/11
Posts: 40
Last seen: 12 years, 2 months
Re: MS syringe to petri dish contam [Re: b4n4n4r4m4]
    #15911571 - 03/06/12 09:42 PM (12 years, 10 months ago)

The syringe was from a reputable vendor and I'm not blaming them. I did use it once prior to inoculating the petri dishes. I didn't replace the little blue plug, I just put the cap on the needle and put it back in the fridge (in a ziploc). Maybe that was a bad idea.  I'm gonna try my own spore print next.

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Offlinepunkrocker292004
i am a liar
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Registered: 12/17/09
Posts: 2,921
Loc: Flag
Last seen: 3 years, 3 months
Re: MS syringe to petri dish contam [Re: Doc_T]
    #15911751 - 03/06/12 10:21 PM (12 years, 10 months ago)

Quote:

Doc_T said:
Control dish was clean, but all three you used the syringe on showed the same contam?
High five dude, you've proven your syringe is bad a couple months ahead of the typical grower.
:highfive:

If there's mycelium, you may be able to transfer some to a clean dish and salvage.

(One control dish isn't 'proof', but it's good enough for me.)




yah hes right:rolleyes:


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