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The following pics are of rye berries innoculated with B+. There were several bags. Most show signs of a green like colored mold. Some have strong mycelium growth, but still have some of this other colored stuff next to it. The bags contain three cups of rye berries to 2-2 1/4 cups water. They were all sterilized in a pressure cooker for 1hr. and let cool for two days before innoculating and to insure no other contanimant may have been present. I saw no growth of anything till I innoculated. The environment was sterile and I flame sterilized the syringe as well as used rubbing alcohol on everything. The bags were each openned individually, innoculated, and then sealed with an impulse sealer. They have been in a closet with the temp at a steady 82 degrees. The last pic is one of an open bag. The mold, if that is what it is, appears to be greenish in color. When I cut bag a powder seem to escape from bag like a dust. The rye berries smell like fermented fruit. Not all bags look like this one. Each pic is a pic of a different bag. The contaminant, if it is one, appears to be the same in all. If this is mold, which one is it and how can I prevent in future. I will have lost all my bags due to this. The rye berries are organic if that matters. I did not pre soak them. Can mycelium look like mold? Can it start looking greenish like this and then grow to a proper white color? Am I screwed? Could it have been bad syringes? When using rye berries for a bulk substrate, should I always pre-soak? Thanks for any suggestions.
Pic # 1 Grayish to green color - Have not openned to smell
Pic #2 Same thing
Pic #3 Notice this is really green - I have shaken this one up to mix prior to pic
Pic #4 This is the openned bag
Re: Possible rye berry contanimation? Help identify please. [Re: Michsageman] #1591295 - 05/29/03 11:45 PM (15 years, 1 day ago)
How can I prevent in future? This has contanimated every single bag out of thirty. I cleaned the room where innoculation took place and even saturated the air with lysol to clean air. What else could I have done?