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OfflinePsykronik
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1st LC UPDATED *pics*
    #15902466 - 03/05/12 06:26 AM (12 years, 10 months ago)

This is my first LC using light honey and water at a 4% - 5% ratio to water. 3 tubs, been 3 days and still no sign on growth. Anyone offer advice? there is alot of sediment in 1 tub though..

Edited by Psykronik (03/12/12 07:32 AM)

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OfflineJ-baller
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Re: 1st LC *Pics* Advice needed [Re: Psykronik]
    #15902533 - 03/05/12 07:06 AM (12 years, 10 months ago)

3 days is early still. Give it a couple more.

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Offlineayemoner

Registered: 07/17/10
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Re: 1st LC *Pics* Advice needed [Re: Psykronik]
    #15902539 - 03/05/12 07:08 AM (12 years, 10 months ago)

Looks like you used spores? If so where did u get them from? What about Ur temp? Id take the tinfoil off and throw some micropore tape on the hole. Just wait a few more days I usually get something at day 5 or even 7 at times. Good luck


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OfflinePsykronik
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Re: 1st LC *Pics* Advice needed [Re: ayemoner]
    #15902554 - 03/05/12 07:16 AM (12 years, 10 months ago)

Quote:

J-baller said:
3 days is early still. Give it a couple more.



will do :thumbup:
Quote:

ayemoner said:
Looks like you used spores? If so where did u get them from? What about Ur temp? Id take the tinfoil off and throw some micropore tape on the hole. Just wait a few more days I usually get something at day 5 or even 7 at times. Good luck



spores are legit and reliable allready have 4 Brf tubs colonizing now.

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Offlineayemoner


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Re: 1st LC *Pics* Advice needed [Re: Psykronik]
    #15902561 - 03/05/12 07:20 AM (12 years, 10 months ago)

Just wait a few more days and ull be good to go hopefully


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OfflinePsykronik
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Re: 1st LC *Pics* Advice needed [Re: ayemoner]
    #15902580 - 03/05/12 07:30 AM (12 years, 10 months ago)

Quote:

ayemoner said:
Just wait a few more days and ull be good to go hopefully


:thumbup:

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OfflinePsykronik
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Re: 1st LC *Pics* Advice needed [Re: Psykronik]
    #15918647 - 03/08/12 09:51 AM (12 years, 10 months ago)

Ok so just checked on the LC and the spores are surounded in a cloudy substance that seems to be clinging to to them. Im wondering if this is normal or it is contam? I'l post some pix up in the next 10 mins.

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Offlinemister brown 420
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Re: 1st LC *Pics* Advice needed [Re: Psykronik]
    #15918726 - 03/08/12 10:10 AM (12 years, 10 months ago)

Oh ya lol?? I too started a LC for the first time id like to see your pictures.


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OfflineTweakerO
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Re: 1st LC *Pics* Advice needed [Re: Psykronik]
    #15918763 - 03/08/12 10:19 AM (12 years, 10 months ago)

Your probably on your way with germination/mycelium or its contam.  I just went through two MS syringes from a sponsor, Z strain and 6 quart jars of malt/Evap Cane/97% h2o and no growth after 3 weeks. No contams either so I guess I have that to amdire.:confused:

New pics please, Goodluck!

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OfflinePsykronik
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Re: 1st LC *Pics* Advice needed [Re: mister brown 420]
    #15918781 - 03/08/12 10:24 AM (12 years, 10 months ago)

Heres the pics... Best I could do in this light.
[image]http://www.shroomery.org/forums/thumbs/12-10/122730757-thumb_SAM_1486.jpg[/image]

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OfflinePsykronik
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Re: 1st LC *Pics* Advice needed [Re: TweakerO]
    #15918797 - 03/08/12 10:27 AM (12 years, 10 months ago)

Quote:

TweakerO said:
Your probably on your way with germination/mycelium or its contam.  I just went through two MS syringes from a sponsor, Z strain and 6 quart jars of malt/Evap Cane/97% h2o and no growth after 3 weeks. No contams either so I guess I have that to amdire.:confused:

New pics please, Goodluck!



Yeh I think its one of the 2 :thumbup:.
Anyone got any experience on identifying contams and mycelium and know the difference please give it a look

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OfflinePsykronik
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Re: 1st LC *Pics* Advice needed [Re: Psykronik]
    #15918901 - 03/08/12 10:49 AM (12 years, 10 months ago)

no one know?

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OfflinePsykronik
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Re: 1st LC *Pics* Advice needed [Re: Psykronik]
    #15919200 - 03/08/12 12:01 PM (12 years, 10 months ago)

*bump* Might throw out as I've got my doubts

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Invisiblepsychotropical

Registered: 10/15/11
Posts: 235
Re: 1st LC *Pics* Advice needed [Re: Psykronik]
    #15920139 - 03/08/12 03:12 PM (12 years, 10 months ago)

I've only done a little LC but it looks like spores germinating to me. I'd wait to get some more advice from some experienced LCers here before tossing...

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Offlinenumnum59
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Re: 1st LC *Pics* Advice needed [Re: psychotropical]
    #15920338 - 03/08/12 03:49 PM (12 years, 10 months ago)

Ime its normal, those are spores with myc growing good luck

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OfflinePsykronik
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Re: 1st LC *Pics* Advice needed [Re: numnum59]
    #15920541 - 03/08/12 04:26 PM (12 years, 10 months ago)

I will let them grow and see how it goes then cheers.

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Offlinetorque
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Re: 1st LC *Pics* Advice needed [Re: Psykronik]
    #15920604 - 03/08/12 04:39 PM (12 years, 10 months ago)

Hey, I hope it turns out that what you have is mycelium growing.  Please post more pictures in the next few days as I'm sure it will help anyone who is considering doing LC or already set up to do it themselves.

Again, good luck sir!

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OfflinePsykronik
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Re: 1st LC *Pics* Advice needed [Re: torque]
    #15920826 - 03/08/12 05:16 PM (12 years, 10 months ago)

Yeh I will do, I'll also start a thread when I do a test batch of PFs n use the LC :thumbup:

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Offlinetorque
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Re: 1st LC *Pics* Advice needed [Re: Psykronik]
    #15923610 - 03/09/12 09:11 AM (12 years, 10 months ago)

Awesome thanks man, I am looking to start LC very soon so any kind of posts about it will be helpful.  I think a lot of people are afraid of showing failure in picture form here, but truthfully, those are the most helpful pictures for people who haven't gone through it themselves yet!

Good vibes sir~

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OfflineQueen of Kings
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Re: 1st LC *Pics* Advice needed [Re: torque]
    #15923680 - 03/09/12 09:29 AM (12 years, 10 months ago)

The first picture looks good.  But pics 3 and 4 show some kind of dark thing in the center of the blobs.  I don't think that should be there; the blobs should be clear.  Unless it's some kind of sediment or you put in a bucket of spores and they're clumping.  Give it some time to keep growing.  You need only a knife-tip of spores tipped in to make a fine liquid culture.  Swirl the mixture every time you walk past it to break up the clumps.  Good luck!

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OfflineQueen of Kings
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Re: 1st LC *Pics* Advice needed [Re: Queen of Kings]
    #15923684 - 03/09/12 09:30 AM (12 years, 10 months ago)


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Offlineyoungdout
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Re: 1st LC *Pics* Advice needed [Re: Queen of Kings]
    #15924155 - 03/09/12 11:19 AM (12 years, 10 months ago)

in the first couple days did your LC look like tiny crystal hairs that glisten in the sun when you stirred them around?

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OfflinePsykronik
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Re: 1st LC *Pics* Advice needed [Re: Queen of Kings]
    #15924158 - 03/09/12 11:21 AM (12 years, 10 months ago)

Quote:

Michelle M said:
The first picture looks good.  But pics 3 and 4 show some kind of dark thing in the center of the blobs.  I don't think that should be there; the blobs should be clear.  Unless it's some kind of sediment or you put in a bucket of spores and they're clumping.  Give it some time to keep growing.  You need only a knife-tip of spores tipped in to make a fine liquid culture.  Swirl the mixture every time you walk past it to break up the clumps.  Good luck!



Yeh those are spores it was only about a quarter ml of a MS syringe maybe not even that much. There is a fair amount of sediment in them also thats down to me not filtering my honey but from what I've seen it gives the myc something to grow on. When will I be able to use the LC btw

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OfflinePsykronik
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Re: 1st LC *Pics* Advice needed [Re: Psykronik]
    #15936570 - 03/12/12 07:29 AM (12 years, 10 months ago)

9 days in now and this is what its looking like. Just not sure its viable, as it doesn't ressemble anyone elses LC really lol, although strict sterile procedures were adhered to.



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