Home | Community | Message Board

Mycohaus
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   PhytoExtractum Buy Bali Kratom Powder   MagicBag.co Certified Organic All-In-One Grow Bags by Magic Bag   Myyco.com Golden Teacher Liquid Culture For Sale   North Spore Injection Grain Bag   Original Sensible Seeds High THC Strains   Left Coast Kratom Buy Kratom Extract   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)

Jump to first unread post Pages: 1 | 2 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflinewerDehT
Offset
Male

Registered: 12/15/11
Posts: 707
Loc: Over the cuckoo's nest
Last seen: 2 years, 11 months
Germination near standing water...
    #15891975 - 03/02/12 07:16 PM (12 years, 10 months ago)

Oh man I'm steamin mad at my own stupidity. I use the brf substitute for agar that I found on this site to germinate my shiot on, then transfer a wedge to my grains. It's always worked well for me. Here's the problem...->made me some new lids, u know with the self healing port. I figure that after I took them out of the pc once they returned o room temp the pressure would equalize back to normal. Boy was I wrong.

2cc's were sucked right into the jar.

I realized the obvious answer is a re-do of the whole deal, sadly that was my last 2cc's of that strain soooooo I propped it up on its side so some of the 'agar' is exposed to air (about 3/4 of it). But there is a small puddle in the corner. Am I wasting my time by waiting to see what happens? Im really hoping I'll get some growth on the one side so I can transfer to a jar done right.


--------------------
"It's only after you've lost everything that your free to do anything."

Extras: Filter Print Post Top
Invisibleskuzzy1232
:))
Male User Gallery


Registered: 05/31/10
Posts: 1,460
Re: Germination near standing water... [Re: werDehT]
    #15892078 - 03/02/12 07:42 PM (12 years, 10 months ago)

2cc's is the optimum ammount that your supposed to add.:shrug:


--------------------
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8468463
Have any questions comments PM me ill try to help out the only stupid question is the one you dont ask .

Extras: Filter Print Post Top
OfflinewerDehT
Offset
Male

Registered: 12/15/11
Posts: 707
Loc: Over the cuckoo's nest
Last seen: 2 years, 11 months
Re: Germination near standing water... [Re: skuzzy1232]
    #15892250 - 03/02/12 08:20 PM (12 years, 10 months ago)

Really? Theyre the tall narrow 1/2 pint'ers. Maybe it was more then cus there's about 1/8 in. Of solution when it's sitting flat.


--------------------
"It's only after you've lost everything that your free to do anything."

Extras: Filter Print Post Top
Invisibleskuzzy1232
:))
Male User Gallery


Registered: 05/31/10
Posts: 1,460
Re: Germination near standing water... [Re: werDehT]
    #15892595 - 03/02/12 09:55 PM (12 years, 10 months ago)

oh .... just keep turning them opposite sides and even upside down and you should be fine man ....


--------------------
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8468463
Have any questions comments PM me ill try to help out the only stupid question is the one you dont ask .

Extras: Filter Print Post Top
OfflinewerDehT
Offset
Male

Registered: 12/15/11
Posts: 707
Loc: Over the cuckoo's nest
Last seen: 2 years, 11 months
Re: Germination near standing water... [Re: skuzzy1232]
    #15892654 - 03/02/12 10:12 PM (12 years, 10 months ago)

K thanks dude


--------------------
"It's only after you've lost everything that your free to do anything."

Extras: Filter Print Post Top
Invisibleskuzzy1232
:))
Male User Gallery


Registered: 05/31/10
Posts: 1,460
Re: Germination near standing water... [Re: werDehT]
    #15892711 - 03/02/12 10:28 PM (12 years, 10 months ago)

:rockon:


--------------------
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8468463
Have any questions comments PM me ill try to help out the only stupid question is the one you dont ask .

Extras: Filter Print Post Top
Invisibleskuzzy1232
:))
Male User Gallery


Registered: 05/31/10
Posts: 1,460
Re: Germination near standing water... [Re: skuzzy1232]
    #15892713 - 03/02/12 10:28 PM (12 years, 10 months ago)

pm me anytime


--------------------
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8468463
Have any questions comments PM me ill try to help out the only stupid question is the one you dont ask .

Extras: Filter Print Post Top
Offlineshamanamba
The Shaman
Male User Gallery

Registered: 06/15/05
Posts: 1,122
Last seen: 2 months, 12 days
Trusted Cultivator
Re: Germination near standing water... [Re: werDehT]
    #15892821 - 03/02/12 11:04 PM (12 years, 10 months ago)

Quote:

werDehT said:
2cc's were sucked right into the jar.





You had residual negative pressure in a grain jar??


--------------------
The Shaman

Extras: Filter Print Post Top
OfflinewerDehT
Offset
Male

Registered: 12/15/11
Posts: 707
Loc: Over the cuckoo's nest
Last seen: 2 years, 11 months
Re: Germination near standing water... [Re: shamanamba]
    #15894024 - 03/03/12 10:06 AM (12 years, 10 months ago)

Try reading again lol


--------------------
"It's only after you've lost everything that your free to do anything."

Extras: Filter Print Post Top
OfflinewerDehT
Offset
Male

Registered: 12/15/11
Posts: 707
Loc: Over the cuckoo's nest
Last seen: 2 years, 11 months
Re: Germination near standing water... [Re: skuzzy1232]
    #15894499 - 03/03/12 12:36 PM (12 years, 10 months ago)

Beautiful tat btw Skuzzy. Im hopin to get this on my side soon.



--------------------
"It's only after you've lost everything that your free to do anything."

Extras: Filter Print Post Top
Invisibleskuzzy1232
:))
Male User Gallery


Registered: 05/31/10
Posts: 1,460
Re: Germination near standing water... [Re: werDehT]
    #15895343 - 03/03/12 04:24 PM (12 years, 10 months ago)

thats  whats up bro im doing a shroom sleeve on my leg  thanks .


--------------------
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8468463
Have any questions comments PM me ill try to help out the only stupid question is the one you dont ask .

Extras: Filter Print Post Top
InvisibleInfinitys Minute
a universe inside each moment
I'm a teapot


Registered: 11/02/11
Posts: 1,757
Loc: Zion
x [Re: skuzzy1232]
    #15895635 - 03/03/12 05:27 PM (12 years, 10 months ago)

x


--------------------
All my posts are completely fictional and are for entertainment purposes only, images I use, post or reference on this account are sourced from the internet and are not my own.

Edited by Infinitys Minute (10/04/16 02:17 AM)

Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 7 days
Trusted Cultivator
Re: Germination near standing water... [Re: werDehT]
    #15895768 - 03/03/12 06:06 PM (12 years, 10 months ago)

I would just try to keep that puddle to one side so the spores have a place to germinate and transfer some mycelium to a new jar ASAP.


--------------------

Extras: Filter Print Post Top
OfflinewerDehT
Offset
Male

Registered: 12/15/11
Posts: 707
Loc: Over the cuckoo's nest
Last seen: 2 years, 11 months
Re: Germination near standing water... [Re: Infinitys Minute]
    #15895802 - 03/03/12 06:15 PM (12 years, 10 months ago)

Sry for the confusion. I'm using this tek...

http://www.shroomery.org/8514/Agar-substitute

Yes it's a thin Layer of brf, no verm. And your correct, the jar is just a sub for the petri dish.

I equalized the pressure using that method for the other 2 strains I was working with unfortunately the first jar took all that was left of that syringe. Afterward I equalized the pressure in that jar I'm just hoping I'll get some growth in it even though there's standing water in the jar. I have them stored on tipped to they're side so about 3/4 of the brf  is exposed to air.


--------------------
"It's only after you've lost everything that your free to do anything."

Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 7 days
Trusted Cultivator
Re: Germination near standing water... [Re: werDehT]
    #15895942 - 03/03/12 06:49 PM (12 years, 10 months ago)

Why don't you just add a polyfill or tyvek filter to the lids so you don't haven't to worry about the pressure.


--------------------

Extras: Filter Print Post Top
OfflinewerDehT
Offset
Male

Registered: 12/15/11
Posts: 707
Loc: Over the cuckoo's nest
Last seen: 2 years, 11 months
Re: Germination near standing water... [Re: Kizzle]
    #15895996 - 03/03/12 07:01 PM (12 years, 10 months ago)

Kizzle u don't understand the situation. Reading my posts will fix that right up though.


--------------------
"It's only after you've lost everything that your free to do anything."

Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 7 days
Trusted Cultivator
Re: Germination near standing water... [Re: werDehT]
    #15896084 - 03/03/12 07:26 PM (12 years, 10 months ago)

I read them. I've done the exact same thing with the BRF agar substitute in half-pint jars. If you would have had a filter on there in addition to the self sealing port the pressure would have equalized on it's own before you even inoculated them.


--------------------

Extras: Filter Print Post Top
Invisibleskuzzy1232
:))
Male User Gallery


Registered: 05/31/10
Posts: 1,460
Re: Germination near standing water... [Re: Infinitys Minute]
    #15896225 - 03/03/12 07:55 PM (12 years, 10 months ago)

Quote:

Infinitys Minute said:
Quote:

shamanamba said:
You had residual negative pressure in a grain jar??



AFAIK he has a SHIP but no GE port, and is using a jar as a petri dish alternative.

Quote:

skuzzy1232 said:
2cc's is the optimum ammount that your supposed to add.:shrug:



You sure? OP's not making 1/2 pint PF jars here... we're talking about a much smaller volume of BRF + verm mix in the jar (assuming this is the case, at least).

Not saying you're wrong at all, especially as I've never done this.
But just saying if 1-2cc per 1/2 pint PF tek is good, it would seem off if it was also a good amount for a far smaller amount of BRF mix.

Also there's some assumption here on my part - OP didn't explicitly state they were using it in a very thin layer... But I assume they are based on the way they said they were using it as an agar substitute, and I believe that's what the tek in question calls for.



Seems to be some confusion in this thread. If not then it's just me who's confused :tongue:
Care to clear that one up, OP? Also, for the record, if your jars have no GE port to equalise pressure, remove the plunger from a syringe, apply a suitable filter medium (eg. stuff with polyfil), sterilise it, flame the needle and then pierce the SHIP with it. This will prevent your jar automatically emptying your syringe.


\
sorry i assumed they were quart jars i just skimmed ...


--------------------
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8468463
Have any questions comments PM me ill try to help out the only stupid question is the one you dont ask .

Extras: Filter Print Post Top
OfflinewerDehT
Offset
Male

Registered: 12/15/11
Posts: 707
Loc: Over the cuckoo's nest
Last seen: 2 years, 11 months
Re: Germination near standing water... [Re: skuzzy1232]
    #15896462 - 03/03/12 08:50 PM (12 years, 10 months ago)

Right I know what I did wrong and how to do it right, thank you though. My question was will I get any growth in the one that I messed up.


--------------------
"It's only after you've lost everything that your free to do anything."

Extras: Filter Print Post Top
InvisibleInfinitys Minute
a universe inside each moment
I'm a teapot


Registered: 11/02/11
Posts: 1,757
Loc: Zion
x [Re: werDehT]
    #15896752 - 03/03/12 10:12 PM (12 years, 10 months ago)

x


--------------------
All my posts are completely fictional and are for entertainment purposes only, images I use, post or reference on this account are sourced from the internet and are not my own.

Edited by Infinitys Minute (10/04/16 02:27 AM)

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | Next >  [ show all ]

Shop: Mushroom-Hut Liquid Cultures   PhytoExtractum Buy Bali Kratom Powder   MagicBag.co Certified Organic All-In-One Grow Bags by Magic Bag   Myyco.com Golden Teacher Liquid Culture For Sale   North Spore Injection Grain Bag   Original Sensible Seeds High THC Strains   Left Coast Kratom Buy Kratom Extract   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)


Similar ThreadsPosterViewsRepliesLast post
* G2G transfers
( 1 2 all )
kjb1891 33,625 20 12/19/18 11:45 AM
by Nichrome
* G2G Transfer? lvleph 4,557 9 11/20/02 12:28 PM
by scifi5001
* substrate transfer?!?! dets 3,017 8 11/13/02 12:29 AM
by SixTango
* G2G transfers? lvleph 4,280 15 11/03/13 06:15 AM
by SpitballJedi
* Ideas for 'ingredient' substitutions DarK_SavioR 2,016 6 05/12/01 09:56 AM
by MAIA
* germination period l33tflip1 404 2 04/15/06 08:21 AM
by l33tflip1
* substitute for agar? brewwhaha1 2,349 15 02/19/05 09:27 PM
by MontyRev
* Will pan cyans germinate on rye? Rhizoid 5,129 7 03/08/07 10:14 AM
by Rhizoid

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
782 topic views. 17 members, 78 guests and 19 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.007 seconds on 14 queries.