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Invisibleslushie9090

Registered: 08/14/01
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Cracked jars,spore syringe fountain and messy inoculation....the joys of a first attempt *DELETED* *DELETED*
    #15893761 - 03/03/12 08:09 AM (12 years, 10 months ago)

Post deleted by Rin

Reason for deletion: .

Edited by slushie9090 (03/03/12 08:18 AM)

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OfflineSomeGuy
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Re: Cracked jars,spore syringe fountain and messy inoculation....the joys of a first attempt [Re: slushie9090]
    #15893774 - 03/03/12 08:15 AM (12 years, 10 months ago)

:facepalm3: I guess you gained experience. That'll help you next time. :cookiemonster:

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OfflineHybridprX
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Re: Cracked jars,spore syringe fountain and messy inoculation....the joys of a first attempt [Re: SomeGuy]
    #15893800 - 03/03/12 08:27 AM (12 years, 10 months ago)

Thats alright friend, My very first attempt(s) I inoculated the pf cakes and then boiled them, 2nd attempt I boiled them but didnt let them cool down before inoculating.... 3rd attempt I lit both my arms on fire in a glovebox (That was the funniest thing in my life to date)

It is a learning experience :wink:

and by the way, since you say your jars cracked....there is a could possibility that you may have dried out the substrate when the pot ran dry.


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OfflineSomeGuy
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Re: Cracked jars,spore syringe fountain and messy inoculation....the joys of a first attempt [Re: HybridprX]
    #15893806 - 03/03/12 08:30 AM (12 years, 10 months ago)

the trick to getting your needle red hot is to tip it almost straight up so damn near the whole thing is in the flame, if you hold the syringe sideways, you'll never get the whole thing hot at one time :lecture:
    It doesn't sound very hopeful, but what-do-ya-do?

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OfflineHybridprX
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Re: Cracked jars,spore syringe fountain and messy inoculation....the joys of a first attempt [Re: SomeGuy]
    #15893811 - 03/03/12 08:34 AM (12 years, 10 months ago)

I should have added that you do not need to get the needle red hot before each inoculation.

I never, ever flame sterilize my syringe tips, simply giving it a wipe with a alcohol soaked paper towel is sufficient.... or just run a regular flame up and down the needle a couple of times, that is sufficient.

and I do my pf cakes in open air without contams so.....maybe I have special cultivation powers?


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InvisibleEvilMushroom666
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Re: Cracked jars,spore syringe fountain and messy inoculation....the joys of a first attempt [Re: HybridprX]
    #15893839 - 03/03/12 08:50 AM (12 years, 10 months ago)

Quote:

HybridprX said:
I never, ever flame sterilize my syringe tips, simply giving it a wipe with a alcohol soaked paper towel is sufficient.... or just run a regular flame up and down the needle a couple of times, that is sufficient.





I mean no offense but that is not only terrible advice to give to someone
starting out, but a terrible practice that falls far from aseptic technique.

If you are having issues getting your needle red hot I would suggest either
a small butane torch lighter, or a small propane plumbing torch, your
needle will be red hot and sterilized within a few seconds.

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OfflineDurban.Poison
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Re: Cracked jars,spore syringe fountain and messy inoculation....the joys of a first attempt [Re: HybridprX]
    #15893855 - 03/03/12 08:56 AM (12 years, 10 months ago)

Your pot boiling dry could have been two factors...1) your lid doesn't fit tight enough...you could remedy this by adding foil to the top of your pot, or 2) you didnt turn down the heat low enough after it came to a boil...dont be scared to turn it down too low...it doesn't take much to keep it going...I have a gas stove and I turn it down as low as it will go

try experimenting with boiling water without anything in it...wouldn't think boiling water would need practice huh...haha

as for your jars cracking...put your jars and room temperature tap water in at the same time and then put it to the heat....dont add jars to boiling pot...this is a sure fire way to crack them

as for the syringe requiring alot of pressure...this could happen if your syringe has alot of clumps in it and you don't shake it well enough and they are clogging the tip...make sure you shake with the cap on, because I have shook so hard I shook out about a 1/2 cc before

when I inoculate, I use my index finger and thumb on the side of the plunger just where it meets the syringe...so the possibility of your range of motion is very limited...I did the whole blasting spores up in the air my first time too

hope this help...better luck next time

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OfflineHybridprX
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Re: Cracked jars,spore syringe fountain and messy inoculation....the joys of a first attempt [Re: EvilMushroom666]
    #15893859 - 03/03/12 08:59 AM (12 years, 10 months ago)

Unless someone is leaving their needles uncovered, laying on dust and dirt or poking bacteria and mold with them, there is no chance that the steel will harbour mold spores or bacteria from the time its used to suck up boiling or distilled water.

Im sorry but Ive never had a contaminate introduced from the syringe unless it was the print itself that was contaminated.

Also, when steel is heated to the point it glows red, it draws in the surrounding air when cooling, so you're actually making a mold spore magnet by heating it outside of a flowhood or glovebox.


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OfflineDurban.Poison
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Re: Cracked jars,spore syringe fountain and messy inoculation....the joys of a first attempt [Re: Durban.Poison]
    #15893870 - 03/03/12 09:04 AM (12 years, 10 months ago)

I left out a comment on flame sterilizing the needle...just use the lighter you have and hold the needle tip about 1/2 inch above the tallest part of the flame...not directly in it...should take less than 5 seconds to be red hot...experiment with this on an old syringe before you get in the pressure situation next time

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InvisibleEvilMushroom666
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Re: Cracked jars,spore syringe fountain and messy inoculation....the joys of a first attempt [Re: HybridprX]
    #15893891 - 03/03/12 09:12 AM (12 years, 10 months ago)

Quote:

HybridprX said:
Unless someone is leaving their needles uncovered, laying on dust and dirt or poking bacteria and mold with them, there is no chance that the steel will harbour mold spores or bacteria from the time its used to suck up boiling or distilled water.

Im sorry but Ive never had a contaminate introduced from the syringe unless it was the print itself that was contaminated.

Also, when steel is heated to the point it glows red, it draws in the surrounding air when cooling, so you're actually making a mold spore magnet by heating it outside of a flowhood or glovebox.




I disagree with you 100%.

Just because I have sex without a condom and have never gotten a women
pregnant does not mean I go around telling others that it is 100% fine
to practice unsafe sex. Just because it has worked for me, does not mean
that it will work for others, just ask my friend John who has three kids.

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Invisibleslushie9090

Registered: 08/14/01
Posts: 2,000
Re: Cracked jars,spore syringe fountain and messy inoculation....the joys of a first attempt *DELETED* [Re: EvilMushroom666]
    #15893910 - 03/03/12 09:22 AM (12 years, 10 months ago)

Post deleted by Rin

Reason for deletion: ?


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InvisibleEvilMushroom666
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Re: Cracked jars,spore syringe fountain and messy inoculation....the joys of a first attempt [Re: slushie9090]
    #15893927 - 03/03/12 09:28 AM (12 years, 10 months ago)

I do not mean to speak off topic Rin, I do apologize.

It just burns my gears when someone gives TERRIBLE advice from personal
experience to someone new to the hobby. Not everyone will have the same
spore load, environment, climate, materials, etc so to say "Well being
lazy with my sterile procedure has never bitten me in the ass, you should
go ahead and do the same" is beyond retarded IMO. Sterile/Aseptic Technique
is a standard for a reason, so that people from all over can replicate
the same environment and set of variables to achieve success with their
experiments and projects.


As for your questions in regards to steam sterilizing your jars, as
said above you need to make sure your lid is very tight, and after bringing
your water to a foil boil, turn the temperature down to a rolling boil
and start your time from then.

Perhaps taking a look through the link in my signature in regards to my
take on BRF cakes will give you some more insight into how I go about
doing things. You can perhaps take this information, apply it to your
own situation and find the solution to your problems.

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Invisibleslushie9090

Registered: 08/14/01
Posts: 2,000
Re: Cracked jars,spore syringe fountain and messy inoculation....the joys of a first attempt *DELETED* [Re: EvilMushroom666]
    #15893952 - 03/03/12 09:38 AM (12 years, 10 months ago)

Post deleted by Rin

Reason for deletion: ?


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Invisible13shroomsM
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Re: Cracked jars,spore syringe fountain and messy inoculation....the joys of a first attempt [Re: HybridprX]
    #15894016 - 03/03/12 10:03 AM (12 years, 10 months ago)

Quote:

HybridprX said:
Unless someone is leaving their needles uncovered, laying on dust and dirt or poking bacteria and mold with them, there is no chance that the steel will harbour mold spores or bacteria from the time its used to suck up boiling or distilled water.

Im sorry but Ive never had a contaminate introduced from the syringe unless it was the print itself that was contaminated.

Also, when steel is heated to the point it glows red, it draws in the surrounding air when cooling, so you're actually making a mold spore magnet by heating it outside of a flowhood or glovebox.





:rolleyes:

now we know why he USED to have a TC tag :smirk:

you are just plan out giving bad advice, even if it does work for you you should still help others by giving the best advice possible not just how to cut corners and get by with luck. :nono:


kinda reminds me of how Doc_Ts gives advice. :thumbdown:

:themoreyouknow:


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OfflineHybridprX
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Re: Cracked jars,spore syringe fountain and messy inoculation....the joys of a first attempt [Re: 13shrooms]
    #15894046 - 03/03/12 10:15 AM (12 years, 10 months ago)

I dont mean to derail this thread any further but just because I dont agree with RR 100% of the time does not make it bad advice.

The fact of the matter is this, if the syringe is sterilized and then capped, the needle will not harbour contamination unless your poking bacteria and mold with it, period!

and physics says that you are all wrong for thinking that a glowing hot piece of steel does not pull in surrounding air. If you want to play it (excessively) safe, then sure, run a flame up and down the needle a few times but not until it glows red.

The excess heat expands the plastic connecting it to the syringe, and then while its pulling in air, guess what can happen?

Im sorry but I know more people that have fucked up pf cakes inoculations going by the books than those that use common sense with those books.

So again, as long as those syringes are sterilized properly prior to adding spores, they will remain sterile unless you get a bad print or pull in some bunk air by over heating the syringe.


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OfflineSomeGuy
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Re: Cracked jars,spore syringe fountain and messy inoculation....the joys of a first attempt [Re: HybridprX]
    #15894071 - 03/03/12 10:26 AM (12 years, 10 months ago)

Quote:

HybridprX said:
The fact of the matter is this, if the syringe is sterilized and then capped, the needle will not harbour contamination unless your poking bacteria and mold with it, period!


bacteria and mold spores have mass and therefor are effected by gravity. They are always being stirred up and then falling. In that split second between wiping the needle and capping it, spores and bac. can land on it.
[Quote}
and physics says that you are all wrong for thinking that a glowing hot piece of steel does not pull in surrounding air. If you want to play it (excessively) safe, then sure, run a flame up and down the needle a few times but not until it glows red.


Actually since the needle typically has a bit of water/moisture, the heating would cause  steam to blow out, I seriously doubt anything can be sucked in whilst steam is coming out
Quote:


The excess heat expands the plastic connecting it to the syringe, and then while its pulling in air, guess what can happen?


it the connection got tighter when it expanded, unless you melted it
Quote:


Im sorry but I know more people that have fucked up pf cakes inoculations going by the books than those that use common sense with those books.

So again, as long as those syringes are sterilized properly prior to adding spores, they will remain sterile unless you get a bad print or pull in some bunk air by over heating the syringe.



To automatically assume you know more than the experts is bad logic

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Offlinetotal
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Re: Cracked jars,spore syringe fountain and messy inoculation....the joys of a first attempt [Re: HybridprX]
    #15894078 - 03/03/12 10:29 AM (12 years, 10 months ago)

:facepalm:
I would disregard anything HybridprX is saying...:rolleyes:
The flaming red hot needle is not enough to cause a vacuum effect on the rest of the cool syringe...:nonono:
Like Evil said, telling someone not to do this, is retarded...

Go ahead and come back and tell me i am wrong now, even though ive been doing this since the better part of you dripped down your daddy's leg...

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Invisible13shroomsM
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Re: Cracked jars,spore syringe fountain and messy inoculation....the joys of a first attempt [Re: HybridprX]
    #15894085 - 03/03/12 10:32 AM (12 years, 10 months ago)

Quote:

HybridprX said:
I dont mean to derail this thread any further but just because I dont agree with RR 100% of the time does not make it bad advice.

The fact of the matter is this, if the syringe is sterilized and then capped, the needle will not harbour contamination unless your poking bacteria and mold with it, period!

and physics says that you are all wrong for thinking that a glowing hot piece of steel does not pull in surrounding air. If you want to play it (excessively) safe, then sure, run a flame up and down the needle a few times but not until it glows red.

The excess heat expands the plastic connecting it to the syringe, and then while its pulling in air, guess what can happen?

Im sorry but I know more people that have fucked up pf cakes inoculations going by the books than those that use common sense with those books.

So again, as long as those syringes are sterilized properly prior to adding spores, they will remain sterile unless you get a bad print or pull in some bunk air by over heating the syringe.




or until you inoc a jar them move on to the next jar :smirk:

when you flame the needle is still hot when you inoc, so its not cooling to pull in any air, it cools when the 1st drops of spore solution pass through the needle inside the jar, and you are poking into airborn bacteria that settles on your lids each time you inject into your jars.  thats why you flame between each jar. :thumbup:

yes you can wipe the inoc point with alcohol but wiping your needle with it does not sterilize it only sanitizes it briefly . :yesnod:

why take the extra chances/risks if you dont need to?  its not like its harder. :smirk:

giving advice is to give them the most successful way to do something not how to cheat and cut corners just to hope it worked for them. :smirk:

sounds like you have a RR stigma :wow:

I think the OP is fine listening to evil666 now anyway. :super:


:plur:


--------------------
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Click here ^ for the AMU forum
VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV
"Man is the sex organ of the machine world" ~  Marshall McLuhan

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Offlinetotal
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Re: Cracked jars,spore syringe fountain and messy inoculation....the joys of a first attempt [Re: 13shrooms]
    #15894092 - 03/03/12 10:34 AM (12 years, 10 months ago)

Quote:

13shrooms said:
Sounds like you have a RR stigma :wow:



NWO is brainwashing people to hate what they dont understand...:lol:

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OfflineHybridprX
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Re: Cracked jars,spore syringe fountain and messy inoculation....the joys of a first attempt [Re: total]
    #15894093 - 03/03/12 10:34 AM (12 years, 10 months ago)

I said, if you feel safer, run a flame up and down the needle.... but I think its un-necessary. Ive never had a contam problem while inoculating jars. Like I said, it must be my magical cultivation powers...

And total, Ive been doing this for nine years there budday.

personal insults are not the issue here, my apologies. But my personal opinion on the subject of flame sterilizing the syringe until its "red hot" stands.



--------------------

Edited by HybridprX (03/03/12 11:07 AM)

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