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OfflinewerDehT
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Germination near standing water...
    #15891975 - 03/02/12 07:16 PM (12 years, 10 months ago)

Oh man I'm steamin mad at my own stupidity. I use the brf substitute for agar that I found on this site to germinate my shiot on, then transfer a wedge to my grains. It's always worked well for me. Here's the problem...->made me some new lids, u know with the self healing port. I figure that after I took them out of the pc once they returned o room temp the pressure would equalize back to normal. Boy was I wrong.

2cc's were sucked right into the jar.

I realized the obvious answer is a re-do of the whole deal, sadly that was my last 2cc's of that strain soooooo I propped it up on its side so some of the 'agar' is exposed to air (about 3/4 of it). But there is a small puddle in the corner. Am I wasting my time by waiting to see what happens? Im really hoping I'll get some growth on the one side so I can transfer to a jar done right.


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Invisibleskuzzy1232
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Re: Germination near standing water... [Re: werDehT]
    #15892078 - 03/02/12 07:42 PM (12 years, 10 months ago)

2cc's is the optimum ammount that your supposed to add.:shrug:


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Have any questions comments PM me ill try to help out the only stupid question is the one you dont ask .

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OfflinewerDehT
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Re: Germination near standing water... [Re: skuzzy1232]
    #15892250 - 03/02/12 08:20 PM (12 years, 10 months ago)

Really? Theyre the tall narrow 1/2 pint'ers. Maybe it was more then cus there's about 1/8 in. Of solution when it's sitting flat.


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Invisibleskuzzy1232
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Re: Germination near standing water... [Re: werDehT]
    #15892595 - 03/02/12 09:55 PM (12 years, 10 months ago)

oh .... just keep turning them opposite sides and even upside down and you should be fine man ....


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http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8468463
Have any questions comments PM me ill try to help out the only stupid question is the one you dont ask .

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OfflinewerDehT
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Re: Germination near standing water... [Re: skuzzy1232]
    #15892654 - 03/02/12 10:12 PM (12 years, 10 months ago)

K thanks dude


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Invisibleskuzzy1232
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Re: Germination near standing water... [Re: werDehT]
    #15892711 - 03/02/12 10:28 PM (12 years, 10 months ago)

:rockon:


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Invisibleskuzzy1232
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Re: Germination near standing water... [Re: skuzzy1232]
    #15892713 - 03/02/12 10:28 PM (12 years, 10 months ago)

pm me anytime


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Offlineshamanamba
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Re: Germination near standing water... [Re: werDehT]
    #15892821 - 03/02/12 11:04 PM (12 years, 10 months ago)

Quote:

werDehT said:
2cc's were sucked right into the jar.





You had residual negative pressure in a grain jar??


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OfflinewerDehT
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Re: Germination near standing water... [Re: shamanamba]
    #15894024 - 03/03/12 10:06 AM (12 years, 10 months ago)

Try reading again lol


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OfflinewerDehT
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Re: Germination near standing water... [Re: skuzzy1232]
    #15894499 - 03/03/12 12:36 PM (12 years, 10 months ago)

Beautiful tat btw Skuzzy. Im hopin to get this on my side soon.



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Invisibleskuzzy1232
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Re: Germination near standing water... [Re: werDehT]
    #15895343 - 03/03/12 04:24 PM (12 years, 10 months ago)

thats  whats up bro im doing a shroom sleeve on my leg  thanks .


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http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8468463
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InvisibleInfinitys Minute
a universe inside each moment
I'm a teapot


Registered: 11/02/11
Posts: 1,757
Loc: Zion
x [Re: skuzzy1232]
    #15895635 - 03/03/12 05:27 PM (12 years, 10 months ago)

x


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All my posts are completely fictional and are for entertainment purposes only, images I use, post or reference on this account are sourced from the internet and are not my own.

Edited by Infinitys Minute (10/04/16 02:17 AM)

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OfflineKizzle
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Re: Germination near standing water... [Re: werDehT]
    #15895768 - 03/03/12 06:06 PM (12 years, 10 months ago)

I would just try to keep that puddle to one side so the spores have a place to germinate and transfer some mycelium to a new jar ASAP.


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OfflinewerDehT
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Re: Germination near standing water... [Re: Infinitys Minute]
    #15895802 - 03/03/12 06:15 PM (12 years, 10 months ago)

Sry for the confusion. I'm using this tek...

http://www.shroomery.org/8514/Agar-substitute

Yes it's a thin Layer of brf, no verm. And your correct, the jar is just a sub for the petri dish.

I equalized the pressure using that method for the other 2 strains I was working with unfortunately the first jar took all that was left of that syringe. Afterward I equalized the pressure in that jar I'm just hoping I'll get some growth in it even though there's standing water in the jar. I have them stored on tipped to they're side so about 3/4 of the brf  is exposed to air.


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OfflineKizzle
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Re: Germination near standing water... [Re: werDehT]
    #15895942 - 03/03/12 06:49 PM (12 years, 10 months ago)

Why don't you just add a polyfill or tyvek filter to the lids so you don't haven't to worry about the pressure.


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OfflinewerDehT
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Re: Germination near standing water... [Re: Kizzle]
    #15895996 - 03/03/12 07:01 PM (12 years, 10 months ago)

Kizzle u don't understand the situation. Reading my posts will fix that right up though.


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OfflineKizzle
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Re: Germination near standing water... [Re: werDehT]
    #15896084 - 03/03/12 07:26 PM (12 years, 10 months ago)

I read them. I've done the exact same thing with the BRF agar substitute in half-pint jars. If you would have had a filter on there in addition to the self sealing port the pressure would have equalized on it's own before you even inoculated them.


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Invisibleskuzzy1232
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Re: Germination near standing water... [Re: Infinitys Minute]
    #15896225 - 03/03/12 07:55 PM (12 years, 10 months ago)

Quote:

Infinitys Minute said:
Quote:

shamanamba said:
You had residual negative pressure in a grain jar??



AFAIK he has a SHIP but no GE port, and is using a jar as a petri dish alternative.

Quote:

skuzzy1232 said:
2cc's is the optimum ammount that your supposed to add.:shrug:



You sure? OP's not making 1/2 pint PF jars here... we're talking about a much smaller volume of BRF + verm mix in the jar (assuming this is the case, at least).

Not saying you're wrong at all, especially as I've never done this.
But just saying if 1-2cc per 1/2 pint PF tek is good, it would seem off if it was also a good amount for a far smaller amount of BRF mix.

Also there's some assumption here on my part - OP didn't explicitly state they were using it in a very thin layer... But I assume they are based on the way they said they were using it as an agar substitute, and I believe that's what the tek in question calls for.



Seems to be some confusion in this thread. If not then it's just me who's confused :tongue:
Care to clear that one up, OP? Also, for the record, if your jars have no GE port to equalise pressure, remove the plunger from a syringe, apply a suitable filter medium (eg. stuff with polyfil), sterilise it, flame the needle and then pierce the SHIP with it. This will prevent your jar automatically emptying your syringe.


\
sorry i assumed they were quart jars i just skimmed ...


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http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8468463
Have any questions comments PM me ill try to help out the only stupid question is the one you dont ask .

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OfflinewerDehT
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Re: Germination near standing water... [Re: skuzzy1232]
    #15896462 - 03/03/12 08:50 PM (12 years, 10 months ago)

Right I know what I did wrong and how to do it right, thank you though. My question was will I get any growth in the one that I messed up.


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InvisibleInfinitys Minute
a universe inside each moment
I'm a teapot


Registered: 11/02/11
Posts: 1,757
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x [Re: werDehT]
    #15896752 - 03/03/12 10:12 PM (12 years, 10 months ago)

x


--------------------
All my posts are completely fictional and are for entertainment purposes only, images I use, post or reference on this account are sourced from the internet and are not my own.

Edited by Infinitys Minute (10/04/16 02:27 AM)

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