Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract   MagicBag.co Certified Organic All-In-One Grow Bags   Mushroom-Hut Liquid Cultures   OlympusMyco.com Olympus Myco Bulk Substrate   Myyco.com Golden Teacher Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinemattch1
Monseur

Registered: 05/16/03
Posts: 126
Loc: WV
Last seen: 19 years, 9 months
liquid culture help!
    #1589263 - 05/29/03 03:26 PM (21 years, 19 days ago)

Ive been trying to grow mycelium in some sort of liquid substrate, so that i can make mycelium syringes but ive been having a few problems. 
First off, i tried a suggestion on a post to use Karo light syrup.  I did as it said, but nothing ever showed up, even after a week in the incubator.  I heated the karo/water mix in the microwave to sterilize (the karo seemed to mix in with the water after heating and was not visible.  Maybe the dextrose molecule broke apart into something unusable by spores?)  I just shaved some of the spores from the print into the jar.  No contams showed up, but no mycelium did either.  Next i tried the same medium, but used a small chunk of colonized PF cake to see if the mycelium would grow out from it.  That solution got a little bit cloudy and smelled (contams?), but no strands of mycelium showed up.  Currently im trying another suggestion, honey.  I put about a tablespoon of honey in 3 jars, and sterilized them for 5, 15, and 40 minutes.  I did this to see if maybe the sugars were being broken into unusable parts by the heat.  I have yet to see the results of this experiment.  Any other suggestions as to why this isnt working for me?!?!?  Thanks alot! :confused: 


--------------------
Everything I ask is for informational purposes only. There is no truth to what i say. I am lying.

Extras: Filter Print Post Top
InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: liquid culture help! [Re: mattch1]
    #1589340 - 05/29/03 03:45 PM (21 years, 19 days ago)

Do you have a PC?

6T


--------------------
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~

Extras: Filter Print Post Top
InvisibleZwieback0
Baby Bread
 User Gallery

Registered: 08/23/02
Posts: 3,473
Re: liquid culture help! [Re: SixTango]
    #1589355 - 05/29/03 03:48 PM (21 years, 19 days ago)

Is it normal to do honey tek and after about 5 - 6 days cloudy,white,fluffy, dusty dots appear at the bottom of the jars?

Extras: Filter Print Post Top
Offlinemattch1
Monseur

Registered: 05/16/03
Posts: 126
Loc: WV
Last seen: 19 years, 9 months
Re: liquid culture help! [Re: Zwieback0]
    #1589383 - 05/29/03 03:54 PM (21 years, 19 days ago)

No, i dont have a pressure cooker


--------------------
Everything I ask is for informational purposes only. There is no truth to what i say. I am lying.

Extras: Filter Print Post Top
InvisibleSixTango
Mycota

Registered: 01/21/02
Posts: 1,996
Loc: A little North of Paradis...
Re: liquid culture help! [Re: mattch1]
    #1589570 - 05/29/03 04:35 PM (21 years, 19 days ago)



I only ask, because I am not a fan of microwave sterilization (as - sometimes it does not).

This is what a healthy liquid culture looks like. Mix was 1/2 teaspoon light dry malt. 1/2 teaspoon dextrose to a quart of water, heated in a pot to a mild boil, then strained through coffee filters into a jar, then PC'ed around 15 pounds for 20 minutes.

Those are plated glass shards in the jar bottom (if you wonder). They are there to hel cut up the thick mass of myc - when the jar is agitated - so it does not plug a syringe).

6T  :wink: 


--------------------
~whiskey river rafting, hot tubbing, dirty dancing & spending money on - wild women - having fun & just gonna waste the rest~

Extras: Filter Print Post Top
Anonymous

Re: liquid culture help! [Re: SixTango]
    #1589630 - 05/29/03 04:47 PM (21 years, 19 days ago)

I'm planning on doing this in a couple days and was wondering how long I should steam....the guide I read said 30mins but didn't specify if that was for a pc or not...I was thinking an hour steamed would be good..oh and I'm using honey and spring water btw....any thoughts?

Extras: Filter Print Post Top
Offlinemattch1
Monseur

Registered: 05/16/03
Posts: 126
Loc: WV
Last seen: 19 years, 9 months
Re: liquid culture help! [Re: ]
    #1590681 - 05/29/03 07:57 PM (21 years, 19 days ago)

Has anyone done it with just honey?  I see lots of recipes for malt and dextrose, but will just honey do the job?  I dont have ready access to those ingredients.  Thanks :smile:


--------------------
Everything I ask is for informational purposes only. There is no truth to what i say. I am lying.

Extras: Filter Print Post Top
OfflinePDU
travel kid vs.amerika
 User Gallery

Registered: 12/03/02
Posts: 10,675
Loc: beautiful BC
Last seen: 8 years, 8 months
Re: liquid culture help! [Re: mattch1]
    #1590889 - 05/29/03 08:29 PM (21 years, 19 days ago)

Yes, honey is just fine. Ive used a single pack of honey from mcdonalds per 1/2 pint in the past...its light honey..


--------------------
GO OUTSIDE.

Extras: Filter Print Post Top
Offlinelemunhed
The hustler'shustler

Registered: 05/12/03
Posts: 775
Loc: Ur moms house
Last seen: 18 years, 10 months
Re: liquid culture help! [Re: PDU]
    #1591159 - 05/29/03 09:17 PM (21 years, 19 days ago)

Yes i have some Lipa Yai i did with just honey. I used the shittiest crystalized honey ever. I only sterilized it for 10 minutes... and i didn't incubate it lol. My culture sucks a dick compared to the one in the picture though. You want a 96% water to 4% sugar ratio.... 1tsp per 2 cups is what i used. It took about 3 days to see growth, but Lipa's are supposed to be fast colonizers.


--------------------
You're pompeius, aren't you? I'll make a pompeian of you unless you hold your toungue!

-Tiberius Claudius Nero

Extras: Filter Print Post Top
Anonymous

Re: liquid culture help! [Re: lemunhed]
    #1592227 - 05/30/03 06:04 AM (21 years, 19 days ago)

so would an hour steamed be ok or too much?

Extras: Filter Print Post Top
Anonymous

Re: liquid culture help! [Re: ]
    #1592670 - 05/30/03 10:26 AM (21 years, 18 days ago)

also would it be a good idea too put a ball bearing or marble into the jar for agitating?

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract   MagicBag.co Certified Organic All-In-One Grow Bags   Mushroom-Hut Liquid Cultures   OlympusMyco.com Olympus Myco Bulk Substrate   Myyco.com Golden Teacher Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* Spores, G2G, Liquid Culture, Cloning & Colonization Time.
( 1 2 3 all )
agar 53,383 43 07/08/23 07:04 AM
by Screwup
* Liquid Culture Challenges Leimana 9,675 4 05/31/05 11:33 PM
by Corporal Kielbasa
* How to make a liquid cultures FAST (like two days)!
( 1 2 3 4 all )
Blue Helix 40,660 68 05/26/13 05:12 PM
by Jman420
* 1st Liquid Culture, arrrgh! Seril 14,593 10 02/28/06 08:35 PM
by Limelight_Liver
* Liquid Culture Recipes MrMaddHatter 24,172 13 03/29/05 04:53 AM
by Sbealzebub
* Clear liquid cultures ? mickywilliams2 3,743 11 10/12/05 05:02 PM
by Bamaman
* i can get liquid cultures, but not grain laughingbuddha 2,846 8 03/08/05 12:02 PM
by laughingbuddha
* how to use cardboard cultures to inoculate grain jars and how to use a liquid culture to inoculate lordoftheshroomz 6,588 12 05/08/05 03:55 PM
by lordoftheshroomz

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,745 topic views. 17 members, 97 guests and 36 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.02 seconds spending 0.005 seconds on 12 queries.