Home | Community | Message Board


Mycohaus
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop for:   eBay Lighters   Amazon ½ Pint Jars, Vermiculite

Jump to first unread post. Pages: 1
Offlinedroogie75
frat-boy killa
Registered: 04/05/03
Posts: 20
Loc: Cockneck, N.C.
Last seen: 13 years, 7 months
flame sterilization
    #1587241 - 05/29/03 03:11 AM (13 years, 8 months ago)

Today, I was outside enjoying the nice weather and my neighbor's rabbit came over. He took a fat shit in my lawn and then pondered these questions:

"Ok so I tried the Bic lighter tek to sterilize my syringe and it left a black mess on my needles. What other options do I have besides an alcohol lamp? I have a backpackers stove that runs on white gas...would that work or would it leave a carbon residue like butane?

Also, does the needle need to be glowing red-hot everytime I sterilize?"

That's all the bunny had to say before he hopped off.


--------------------
Whatever I or any soft furry creatures may say, do, or speculate is complete fiction and doesn't exist in reality.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisible40oz
 User Gallery

Registered: 01/19/01
Posts: 30,035
Loc: Sandy Eggo. Ca.
Re: flame sterilization [Re: droogie75]
    #1587295 - 05/29/03 03:33 AM (13 years, 8 months ago)

yes and yes.


--------------------
:pacman: - - - -  :pill: :mushroom2: :pill2: :mushroom2: :regularshroom: :mushroomgrow: :pill: :pill2: :mushroom2: :poison:

:sun::heart::sun:

tiny_rabid_birds said:
"your avatar is dirty."


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMAIA
World-BridgerKartikeya (DftS)
Male User Gallery

Registered: 04/27/01
Posts: 7,275
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 8 months, 20 days
Re: flame sterilization [Re: 40oz]
    #1588193 - 05/29/03 10:03 AM (13 years, 8 months ago)

Use a cotton piece with rubbing alcohol to clean the needle after you flame it.
Maybe you'll get some change in color, which is ok, but not any "black mess" like you say.

MAIA


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: flame sterilization [Re: MAIA]
    #1588530 - 05/29/03 01:20 PM (13 years, 8 months ago)

I have never flame sterlized syringes and so far no probs...I just use alcohol with a cotton ball and go over it a few times


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinepsilo9com
Koh Koh Gadget

Registered: 05/20/03
Posts: 267
Last seen: 13 years, 5 months
Re: flame sterilization [Re: ]
    #1588566 - 05/29/03 01:39 PM (13 years, 8 months ago)

A butane lighter will do the job, and the char is just a pain but won't effect your results.

Here's how I did it:
-Flame until red hot
-Wipe off char with moist Lysol or Clorox wipes until needle is cooled down.
-Inoculate 4 holes in 1jar with about .25cc each
-repeat.

I used the same clorox wipe for 6 jars, letting it sit on top of its case while i was inoculating. After 6 jars the wipe was all dirty with char and some verm so I changed wipes after that.

0% contams so far.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,122
Loc: my room
Last seen: 1 month, 10 days
Re: flame sterilization [Re: psilo9com]
    #1588572 - 05/29/03 01:42 PM (13 years, 8 months ago)

There is no need to sterilize the needle between each jar, as long the only thing you touch with it is the sterile jar holes or foil.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinemattch1
Monseur

Registered: 05/17/03
Posts: 126
Loc: WV
Last seen: 12 years, 5 months
Re: flame sterilization [Re: Anno]
    #1588833 - 05/29/03 03:16 PM (13 years, 8 months ago)

Dont worry about getting red hot. I never have. Why dont u just use alcohol or lysol to wipe them down?


--------------------
Everything I ask is for informational purposes only. There is no truth to what i say. I am lying.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisiblePrisoner#1M
Even Dumber ThanAdvertized!
 User Gallery

Registered: 01/22/03
Posts: 193,252
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: flame sterilization [Re: mattch1]
    #1590745 - 05/29/03 10:07 PM (13 years, 8 months ago)



my prefered method for heat sterilization....but the afore mentioned methods are simple and dont cost $300.....


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinedroogie75
frat-boy killa
Registered: 04/05/03
Posts: 20
Loc: Cockneck, N.C.
Last seen: 13 years, 7 months
Re: flame sterilization [Re: droogie75]
    #1590919 - 05/29/03 10:35 PM (13 years, 8 months ago)

The rabbit used his white gas backpackers stove to sterilize the needle.  He told me that it worked great (it only took ~5 seconds to make the needle glow red). 

2 concerns the bunny expressed:

1) A piece of verm got caught inside the needle, and no matter how much the rabbit tried to "burn it out", it wouldn't.

2) He got a little excited with the efficiency of the stove and burnt a little section of the syringe (the flange that connects the main cylinder to the needle) and when he inoculated the pf jars a little drop of solution would form where the syringe was burned.  However, the bunny noticed -aside from the accident- all the jars got ~1 cc of B+, flame sterilizing after EVERY jar (he's a tad anal retentive).

Should my neighbor's rabbit be worried? :confused: 


--------------------
Whatever I or any soft furry creatures may say, do, or speculate is complete fiction and doesn't exist in reality.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinedroogie75
frat-boy killa
Registered: 04/05/03
Posts: 20
Loc: Cockneck, N.C.
Last seen: 13 years, 7 months
Re: flame sterilization [Re: droogie75]
    #1591724 - 05/30/03 01:22 AM (13 years, 8 months ago)

My neighbor's rabbit (MNR) has me at gunpoint right now, suggesting that I should be obnoxious and BUMP this thread. Sorry guys


--------------------
Whatever I or any soft furry creatures may say, do, or speculate is complete fiction and doesn't exist in reality.


Post Extras: Print Post  Remind Me! Notify Moderator
Anonymous

Re: flame sterilization [Re: droogie75]
    #1592247 - 05/30/03 08:12 AM (13 years, 8 months ago)

well as long as you didn't kill the spores should be fine....just curious has anyone else tried JUST using alcohol swabbing? I did a couple diff innoculations this way and haven't had a prob yet...lot easier and safer than trying too get the needle red hot


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinecwb0805
ambassador of kick your assador

Registered: 05/15/14
Posts: 97
Last seen: 2 years, 14 days
Re: flame sterilization [Re: droogie75]
    #20424456 - 08/15/14 10:05 PM (2 years, 6 months ago)

I always flame sterilize mine between each jar.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleTheEaglesGift
The Nagual


Registered: 04/11/11
Posts: 10,501
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: flame sterilization [Re: Anonymous]
    #20424700 - 08/15/14 11:11 PM (2 years, 6 months ago)

Quote:

Anonymous said:
well as long as you didn't kill the spores should be fine....just curious has anyone else tried JUST using alcohol swabbing? I did a couple diff innoculations this way and haven't had a prob yet...lot easier and safer than trying too get the needle red hot




What's dangerous about trying to get the needle red hot?


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineOgreLokon
Pretty Fun Guy
Male

Registered: 06/30/13
Posts: 512
Last seen: 8 months, 19 days
Re: flame sterilization [Re: cwb0805]
    #20424723 - 08/15/14 11:16 PM (2 years, 6 months ago)

:oldthread:

I use my Zippo


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop for:   eBay Lighters   Amazon ½ Pint Jars, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Inoculation: Flame Sterilization Vs. Alcohol Sterilization... Does it make a difference?
( 1 2 all )
v3nn3m 11,862 25 01/20/11 10:53 PM
by TheCannabisSativa
* How long do you let your needle cool for after flame sterilizing? invitro 1,392 10 12/29/13 12:26 PM
by Gr13nMushDude
* Question about flame sterilization Xobil3 502 3 03/24/08 03:19 PM
by thimself
* Flame sterilization nimbus8 205 3 08/24/13 12:40 PM
by SpitballJedi
* Time it Takes Needle to Cool after Flame Sterilization?
( 1 2 all )
FallenAngel 3,713 21 11/07/11 01:33 AM
by TheHistoryChannel
* Is flame sterilization necessary?
( 1 2 all )
StatuesCryBleeding 2,431 26 09/19/10 04:20 AM
by total
* Flame Sterilization Experiment
( 1 2 3 4 5 all )
EvilMushroom666M 4,195 94 04/29/15 01:50 PM
by bodhisatta
* noob question about gloveboxes and flame steril. efarley 1,217 11 06/03/10 11:36 PM
by thormaxim

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart, Tmethyl
976 topic views. 29 members, 99 guests and 18 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Lil Shop Of Spores
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.039 seconds spending 0.002 seconds on 14 queries.