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Offlinedroogie75
frat-boy killa
Registered: 04/05/03
Posts: 20
Loc: Cockneck, N.C.
Last seen: 14 years, 17 days
flame sterilization
    #1587241 - 05/29/03 03:11 AM (14 years, 1 month ago)

Today, I was outside enjoying the nice weather and my neighbor's rabbit came over. He took a fat shit in my lawn and then pondered these questions:

"Ok so I tried the Bic lighter tek to sterilize my syringe and it left a black mess on my needles. What other options do I have besides an alcohol lamp? I have a backpackers stove that runs on white gas...would that work or would it leave a carbon residue like butane?

Also, does the needle need to be glowing red-hot everytime I sterilize?"

That's all the bunny had to say before he hopped off.


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Whatever I or any soft furry creatures may say, do, or speculate is complete fiction and doesn't exist in reality.


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Invisible40oz
 User Gallery

Registered: 01/19/01
Posts: 30,038
Loc: Sandy Eggo. Ca.
Re: flame sterilization [Re: droogie75]
    #1587295 - 05/29/03 03:33 AM (14 years, 1 month ago)

yes and yes.


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:sun::heart::sun:

tiny_rabid_birds said:
"your avatar is dirty."


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OfflineMAIA
World-BridgerKartikeya (DftS)
Male User Gallery

Registered: 04/27/01
Posts: 7,276
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 1 month, 15 days
Re: flame sterilization [Re: 40oz]
    #1588193 - 05/29/03 10:03 AM (14 years, 1 month ago)

Use a cotton piece with rubbing alcohol to clean the needle after you flame it.
Maybe you'll get some change in color, which is ok, but not any "black mess" like you say.

MAIA


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Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


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Anonymous

Re: flame sterilization [Re: MAIA]
    #1588530 - 05/29/03 01:20 PM (14 years, 1 month ago)

I have never flame sterlized syringes and so far no probs...I just use alcohol with a cotton ball and go over it a few times


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Offlinepsilo9com
Koh Koh Gadget

Registered: 05/20/03
Posts: 267
Last seen: 13 years, 10 months
Re: flame sterilization [Re: ]
    #1588566 - 05/29/03 01:39 PM (14 years, 1 month ago)

A butane lighter will do the job, and the char is just a pain but won't effect your results.

Here's how I did it:
-Flame until red hot
-Wipe off char with moist Lysol or Clorox wipes until needle is cooled down.
-Inoculate 4 holes in 1jar with about .25cc each
-repeat.

I used the same clorox wipe for 6 jars, letting it sit on top of its case while i was inoculating. After 6 jars the wipe was all dirty with char and some verm so I changed wipes after that.

0% contams so far.


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OfflineAnnoA
Experimenter
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Registered: 06/18/99
Posts: 24,123
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Re: flame sterilization [Re: psilo9com]
    #1588572 - 05/29/03 01:42 PM (14 years, 1 month ago)

There is no need to sterilize the needle between each jar, as long the only thing you touch with it is the sterile jar holes or foil.


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Offlinemattch1
Monseur

Registered: 05/17/03
Posts: 126
Loc: WV
Last seen: 12 years, 10 months
Re: flame sterilization [Re: Anno]
    #1588833 - 05/29/03 03:16 PM (14 years, 1 month ago)

Dont worry about getting red hot. I never have. Why dont u just use alcohol or lysol to wipe them down?


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Everything I ask is for informational purposes only. There is no truth to what i say. I am lying.


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InvisiblePrisoner#1
Even Dumber ThanAdvertized!
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Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: flame sterilization [Re: mattch1]
    #1590745 - 05/29/03 10:07 PM (14 years, 1 month ago)



my prefered method for heat sterilization....but the afore mentioned methods are simple and dont cost $300.....


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Offlinedroogie75
frat-boy killa
Registered: 04/05/03
Posts: 20
Loc: Cockneck, N.C.
Last seen: 14 years, 17 days
Re: flame sterilization [Re: droogie75]
    #1590919 - 05/29/03 10:35 PM (14 years, 1 month ago)

The rabbit used his white gas backpackers stove to sterilize the needle.  He told me that it worked great (it only took ~5 seconds to make the needle glow red). 

2 concerns the bunny expressed:

1) A piece of verm got caught inside the needle, and no matter how much the rabbit tried to "burn it out", it wouldn't.

2) He got a little excited with the efficiency of the stove and burnt a little section of the syringe (the flange that connects the main cylinder to the needle) and when he inoculated the pf jars a little drop of solution would form where the syringe was burned.  However, the bunny noticed -aside from the accident- all the jars got ~1 cc of B+, flame sterilizing after EVERY jar (he's a tad anal retentive).

Should my neighbor's rabbit be worried? :confused: 


--------------------
Whatever I or any soft furry creatures may say, do, or speculate is complete fiction and doesn't exist in reality.


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Offlinedroogie75
frat-boy killa
Registered: 04/05/03
Posts: 20
Loc: Cockneck, N.C.
Last seen: 14 years, 17 days
Re: flame sterilization [Re: droogie75]
    #1591724 - 05/30/03 01:22 AM (14 years, 1 month ago)

My neighbor's rabbit (MNR) has me at gunpoint right now, suggesting that I should be obnoxious and BUMP this thread. Sorry guys


--------------------
Whatever I or any soft furry creatures may say, do, or speculate is complete fiction and doesn't exist in reality.


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Anonymous

Re: flame sterilization [Re: droogie75]
    #1592247 - 05/30/03 08:12 AM (14 years, 1 month ago)

well as long as you didn't kill the spores should be fine....just curious has anyone else tried JUST using alcohol swabbing? I did a couple diff innoculations this way and haven't had a prob yet...lot easier and safer than trying too get the needle red hot


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Offlinecwb0805
ambassador of kick your assador

Registered: 05/15/14
Posts: 97
Last seen: 2 years, 5 months
Re: flame sterilization [Re: droogie75]
    #20424456 - 08/15/14 10:05 PM (2 years, 11 months ago)

I always flame sterilize mine between each jar.


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InvisibleTheEaglesGift
The Nagual


Registered: 04/11/11
Posts: 10,534
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: flame sterilization [Re: Anonymous]
    #20424700 - 08/15/14 11:11 PM (2 years, 11 months ago)

Quote:

Anonymous said:
well as long as you didn't kill the spores should be fine....just curious has anyone else tried JUST using alcohol swabbing? I did a couple diff innoculations this way and haven't had a prob yet...lot easier and safer than trying too get the needle red hot




What's dangerous about trying to get the needle red hot?


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OfflineOgreLokon
Pretty Fun Guy
Male

Registered: 06/30/13
Posts: 512
Last seen: 1 year, 1 month
Re: flame sterilization [Re: cwb0805]
    #20424723 - 08/15/14 11:16 PM (2 years, 11 months ago)

:oldthread:

I use my Zippo


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