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Offlinekdmmontana
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Registered: 11/09/10
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Re: TC Colonization Time? [Re: Amanita virosa]
    #15873803 - 02/27/12 11:00 PM (12 years, 10 months ago)

Quote:

Amanita virosa said:
yea, thats some slowwwwwwwwwww growth for two weeks.  Really hard to tell  if sub is  too wet from those pix.  def doesnt look too dry though. could just be one of those cube mysteries. IF  it were me,  though  you can bet I would be cracking one open and checking out the insides;  i mean shit ya got twelve...






There is a clear way to tell this just by looking at the jar. It looks dry and stale and it looks as if the BRF has clumped up.

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InvisibleShroomerInTheRye
Clit Commander
I'm a teapot User Gallery


Registered: 01/12/12
Posts: 13,038
Loc: Themyscira Flag
Re: TC Colonization Time? [Re: Amanita virosa]
    #15874550 - 02/28/12 02:44 AM (12 years, 10 months ago)

It probably is too wet.  I notice little droplets of water on the inside of the jars.  Kind of like when a mirror or window steams up, then cools off.


--------------------
:nyan: <-- Clicky Clicky

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Offlinekdmmontana
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Re: TC Colonization Time? [Re: ShroomerInTheRye]
    #15874833 - 02/28/12 05:57 AM (12 years, 10 months ago)

If your BRF is too coarse and you add too much of it the result will resemble stale bread dough, not what you want. Make sure to mix the substrate well, over and over again and make sure your BRF is properly ground. Let the water sit with the Verm for 10 minutes or so, this helps the dispersion and the Verm can suck the water up.

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InvisibleShroomerInTheRye
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Registered: 01/12/12
Posts: 13,038
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Re: TC Colonization Time? [Re: kdmmontana]
    #15884511 - 03/01/12 04:58 AM (12 years, 10 months ago)

The BRF I used was pretty fine.  I didn't make my own this time, though.  I used some finer grade verm too.  The guy in the video said to try to keep the substrate loose and airy...you don't want to really pat it down (except a little on the sides to keep it clean).

I noticed some new growth in a jar this morning.  One of the jars has some water droplets in the middle of the mycelium.  I kinda started to freak out a little, but the forums said this is normal and means stuff is growing.

Maybe I'm just impatient...


--------------------
:nyan: <-- Clicky Clicky

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OfflineSackofWoe
Flippin' Hippy Candy!
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Re: TC Colonization Time? [Re: ShroomerInTheRye]
    #15885282 - 03/01/12 10:34 AM (12 years, 10 months ago)

2 weeks is a long time with no signs of growth...


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:starwars:

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OfflineAmanita virosa
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Re: TC Colonization Time? [Re: ShroomerInTheRye]
    #15887112 - 03/01/12 06:31 PM (12 years, 10 months ago)

Quote:

ShroomerInTheRye said:
The BRF I used was pretty fine.  I didn't make my own this time, though.  I used some finer grade verm too.  The guy in the video said to try to keep the substrate loose and airy...you don't want to really pat it down (except a little on the sides to keep it clean).

I noticed some new growth in a jar this morning.  One of the jars has some water droplets in the middle of the mycelium.  I kinda started to freak out a little, but the forums said this is normal and means stuff is growing.

Maybe I'm just impatient...




Water is a by product of aerobic respiration.  Glucose+Oxygen=carbon dioxide and Water.  Totally normal and expected.

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InvisibleShroomerInTheRye
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Re: TC Colonization Time? [Re: SackofWoe]
    #15898561 - 03/04/12 10:39 AM (12 years, 10 months ago)

3 weeks in and here's some more pics of what's happening.  The growth seems to have picked up again and the jars feel warm to the touch.  Last week, they felt a tiny bit cooler than room temp.



--------------------
:nyan: <-- Clicky Clicky

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OfflineAmanita virosa
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Re: TC Colonization Time? [Re: ShroomerInTheRye]
    #15899661 - 03/04/12 03:37 PM (12 years, 10 months ago)

still lookin good. 


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Offlinekdmmontana
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Re: TC Colonization Time? [Re: Amanita virosa]
    #15902299 - 03/05/12 04:17 AM (12 years, 10 months ago)

Fantastic. Keep mixing those subtrates and youll have success every time. RR quality:)

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InvisibleShroomerInTheRye
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Registered: 01/12/12
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Re: TC Colonization Time? [Re: kdmmontana]
    #15902336 - 03/05/12 04:51 AM (12 years, 10 months ago)

Thanks everyone for your input!

Now that I think back...the day I inoculated the jars...when I went to put them in the box, one fell out of my hand and landed on top of a few that were already in the box.  Could that have something to do with how long they're taking?  I remember the verm got pretty jostled in the one jar, but I don't know which one.  I just remember finding verm bits in the bottom of the box and on top of some of the jars.


--------------------
:nyan: <-- Clicky Clicky

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OfflineAmanita virosa
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Re: TC Colonization Time? [Re: ShroomerInTheRye]
    #15902405 - 03/05/12 05:52 AM (12 years, 10 months ago)

prolly not... but mycelium is often a mystery.  thats what is simultainously fun and frustrating about growing. Just when you think you figured it all out, the mushroom gods laugh at you...no rush on those jars though; slow is ok...


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InvisibleShroomerInTheRye
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Registered: 01/12/12
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Re: TC Colonization Time? [Re: Amanita virosa]
    #15925763 - 03/09/12 05:49 PM (12 years, 10 months ago)

Stupid question time...

I still have those jars that are showing absolutely no signs of growth at all.  Not even any contamination or anything.  By now, if there was anything in the jars, I'm sure it would have shown.  Do you think I should toss out the insides of those jars and start fresh or could I possibly use them for another batch?

Also...is it normal to have the cakes pull away from the sides of the jars?  As they're colonizing, they're sort of shrinking inside.


--------------------
:nyan: <-- Clicky Clicky

Edited by ShroomerInTheRye (03/09/12 05:50 PM)

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InvisibleBobbySkidz
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Registered: 02/10/12
Posts: 312
Loc: Neptune
Re: TC Colonization Time? [Re: ShroomerInTheRye]
    #15925991 - 03/09/12 06:37 PM (12 years, 10 months ago)

Quote:

ShroomerInTheRye said:


Also...is it normal to have the cakes pull away from the sides of the jars?  As they're colonizing, they're sort of shrinking inside.




Mine do. It is my first time also.

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InvisibleDX2R
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Registered: 03/04/12
Posts: 74
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Re: TC Colonization Time? [Re: BobbySkidz]
    #15926128 - 03/09/12 07:02 PM (12 years, 10 months ago)

Quote:

BobbySkidz said:
Quote:

ShroomerInTheRye said:


Also...is it normal to have the cakes pull away from the sides of the jars?  As they're colonizing, they're sort of shrinking inside.




Mine do. It is my first time also.



Oh yea of course. The myc is feeding and consuming the substrate, which in return causing it to shink a little bit. Perfectly normal. :thumbup:


--------------------
Nature is not simply the random flight of atoms through electromagnetic fields. Nature is not the empty despiritualized lumpen matter that we inherit from modern physics, but it is instead a kind of intelligence, a kind of mind.        Terence Mckenna

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Invisibleaqh
pothead
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Registered: 04/10/11
Posts: 181
Re: TC Colonization Time? [Re: ShroomerInTheRye]
    #15926192 - 03/09/12 07:13 PM (12 years, 10 months ago)

Your substrate looks fine and the fact that the mycelium is now colonizing faster certainly supports this.
So we can rule that out.
Probably just not the best genetics, but it will obviously work out.


--------------------

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Offlinerodent
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Registered: 12/27/02
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Re: TC Colonization Time? [Re: aqh]
    #15928183 - 03/10/12 02:52 AM (12 years, 10 months ago)

Maybe a stupid question, but you inoculated from multiple points in the jar, towards the glass? I'm wondering if they may be colonizing from inside the jar?

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Offlinemrmojoshroomin
All-summa BeenNoddin
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Re: TC Colonization Time? [Re: rodent]
    #15928390 - 03/10/12 05:26 AM (12 years, 10 months ago)

Quote:

rodent said:
Maybe a stupid question, but you inoculated from multiple points in the jar, towards the glass? I'm wondering if they may be colonizing from inside the jar?



as opposed to outside of the jar??? << not trying to be a smart ass but what do you mean?

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InvisibleShroomerInTheRye
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Registered: 01/12/12
Posts: 13,038
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Re: TC Colonization Time? [Re: rodent]
    #15928457 - 03/10/12 06:43 AM (12 years, 10 months ago)

I inoculated the jars with the needle against the glass, not straight up and down.  The videos and posts I've read said you wanna see the liquid from the syringe run down the sides, so that's what I did.


--------------------
:nyan: <-- Clicky Clicky

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Offlinekdmmontana
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Registered: 11/09/10
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Re: TC Colonization Time? [Re: ShroomerInTheRye]
    #15928907 - 03/10/12 10:20 AM (12 years, 10 months ago)

Quote:

ShroomerInTheRye said:
I inoculated the jars with the needle against the glass, not straight up and down.  The videos and posts I've read said you wanna see the liquid from the syringe run down the sides, so that's what I did.




Two weeks is still a long time to wait for germination, I have suggested a bad substrate blend to be the cause because I believe that is why. Spores will eventually germinate of course but consolidation might become a problem later on. A great increase in time from inoculation to germination (2 weeks or more) suggests that the substrate causes some form of issue. Your jars are looking fine at this point, but I would expect issues with consolidation. Of course there is no tell of that, so you will see. Cakes that wont fruit will be the sign of that.

I retract my statement about the jars earlier, as there is no way to see whats wrong or not wrong with them, It was just my assumption.

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Offlinekdmmontana
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Re: TC Colonization Time? [Re: aqh]
    #15928918 - 03/10/12 10:24 AM (12 years, 10 months ago)

Quote:

aqh said:
Your substrate looks fine and the fact that the mycelium is now colonizing faster certainly supports this.
So we can rule that out.
Probably just not the best genetics, but it will obviously work out.




Thats hard to tell from those pictures and I have never experienced a germination time of 2 weeks, ever. I have had 5 days germination, all over. This lottery theory gets to my nerves. I still bet its his substrate and that his cakes will see problems later on due to consolidation. We will see what goes on, but three weeks (or more) for that small growth suggests that there is something wrong.

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Shop: Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Left Coast Kratom Buy Kratom Extract   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work   North Spore Bulk Substrate


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