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InvisibleShroomerInTheRye
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I'm a teapot User Gallery


Registered: 01/12/12
Posts: 13,038
Loc: Themyscira Flag
TC Colonization Time?
    #15865071 - 02/26/12 06:14 AM (12 years, 10 months ago)

About 2 weeks ago, I inoculated 12 jars with TC spores.  Only 4 of the jars have some growth, but it's really slow.  I know it's not a fast strain, but does that seem right?  I got the spores from THE.  I didn't use one of the syringes right away, so I stored it in my fridge for 2 months.  Could that have done it?


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:nyan: <-- Clicky Clicky

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OfflineAmanita virosa
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Re: TC Colonization Time? [Re: ShroomerInTheRye]
    #15865122 - 02/26/12 06:52 AM (12 years, 10 months ago)

two months is a reasonable amount of time to store them  and still have  viability.  what substrate are you using BRF? or some bulk grain?  need more details. when colonization is very slow or stalls out i always begin to suspect bacterial contamination.  cubes dont competed very well with cocci.

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Offlinekdmmontana
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Re: TC Colonization Time? [Re: ShroomerInTheRye]
    #15865126 - 02/26/12 06:54 AM (12 years, 10 months ago)

2 weeks is very long for germination. I would guess this is due to either to compacted substrate, badly mixed substrate, overly wet substrate, to dry substrate etc. Improperly mixed substrates will not perform well.

Also older spores tend to become slower, as Ive read somewhere long ago. Post up some pics of your jars and we can make a diagnosis:)

/KD

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Offlinekdmmontana
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Re: TC Colonization Time? [Re: Amanita virosa]
    #15865130 - 02/26/12 06:57 AM (12 years, 10 months ago)

Quote:

Amanita virosa said:
two months is a reasonable amount of time to store them  and still have  viability.  what substrate are you using BRF? or some bulk grain?  need more details. when colonization is very slow or stalls out i always begin to suspect bacterial contamination.  cubes dont competed very well with cocci.




I have never had bacterial contaminations. If this is the case, I would suspect improper handling and failed sterilization. To my knowledge endospores are not present in any significant amounts in either Verm or BRF.

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InvisibleShroomerInTheRye
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Registered: 01/12/12
Posts: 13,038
Loc: Themyscira Flag
Re: TC Colonization Time? [Re: Amanita virosa]
    #15865147 - 02/26/12 07:04 AM (12 years, 10 months ago)

I'm using BRF cakes and I followed the "Let's Grow Mushrooms" videos on YouTube:


Maybe it's just too cold?  I keep them in a container on top of the cable box and the house temp is around 74F?


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:nyan: <-- Clicky Clicky

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InvisibleShroomerInTheRye
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Registered: 01/12/12
Posts: 13,038
Loc: Themyscira Flag
Re: TC Colonization Time? [Re: kdmmontana]
    #15865150 - 02/26/12 07:06 AM (12 years, 10 months ago)

Quote:

kdmmontana said:
2 weeks is very long for germination. I would guess this is due to either to compacted substrate, badly mixed substrate, overly wet substrate, to dry substrate etc. Improperly mixed substrates will not perform well.

Also older spores tend to become slower, as Ive read somewhere long ago. Post up some pics of your jars and we can make a diagnosis:)

/KD





I'm starting to think that it might be too low temps or substrate that's too moist.  As soon as I can get a better camera, I'll post some pics.  All I have right now is my crappy cell camera.


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Offlinekdmmontana
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Re: TC Colonization Time? [Re: ShroomerInTheRye]
    #15865157 - 02/26/12 07:09 AM (12 years, 10 months ago)

Quote:

ShroomerInTheRye said:
Quote:

kdmmontana said:
2 weeks is very long for germination. I would guess this is due to either to compacted substrate, badly mixed substrate, overly wet substrate, to dry substrate etc. Improperly mixed substrates will not perform well.

Also older spores tend to become slower, as Ive read somewhere long ago. Post up some pics of your jars and we can make a diagnosis:)

/KD





I'm starting to think that it might be too low temps or substrate that's too moist.  As soon as I can get a better camera, I'll post some pics.  All I have right now is my crappy cell camera.




No way. A decrease in temperature will not hamper your jars. Dont make the mistake of heating them up. A lot of growers do this because they expect a massive increase in growth rate when its most likely to lead to problems. Mind that if you heat a jar, the inside will become hotter than the outside air so if you heat it 5 degrees, the inside will become warmer over time.

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OfflineAmanita virosa
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Re: TC Colonization Time? [Re: kdmmontana]
    #15865179 - 02/26/12 07:24 AM (12 years, 10 months ago)

Quote:



I have never had bacterial contaminations. If this is the case, I would suspect improper handling and failed sterilization. To my knowledge endospores are not present in any significant amounts in either Verm or BRF.




brf and verm,  no.  Spore syringes, yes!

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Offlinekdmmontana
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Re: TC Colonization Time? [Re: Amanita virosa]
    #15865196 - 02/26/12 07:33 AM (12 years, 10 months ago)

Those bunk syringes, I hate them.

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InvisibleShroomerInTheRye
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Registered: 01/12/12
Posts: 13,038
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Re: TC Colonization Time? [Re: kdmmontana]
    #15869170 - 02/27/12 04:36 AM (12 years, 10 months ago)

Checked my jars again this morning and found another one with some growth, bringing the total to 5 out of 12.  I'm gonna ask around and see who has a camera I can use for a bit.  Should I worry that the new growth in the jar is along the verm line, or is that normal?  When I injected the spores, I tried to make sure to get somewhere below the verm line, but not completely to the bottom.


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Offlinekdmmontana
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Re: TC Colonization Time? [Re: ShroomerInTheRye]
    #15869182 - 02/27/12 04:49 AM (12 years, 10 months ago)

Growth can occur at various spots depending on where you shot your spores so thats no issue. As long as you have regular mycelium going:) Here is a pic for comparison and another pic where I point out a contaminant that can be common;

Healthy mycelium



Contaminated Jar



If you look inside the red rectangle you can see ropey growth (much like silk..) that is mold and I believe its cobweb. There are a number of other signs of contamination of different kinds in there so stare yourself blind for a while and learn to spot the difference:)


http://www.shroomery.org/forums/showflat.php/Number/15868913
You can look here for more info, DL the pictures so you can zoom them:)
/KD

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OfflineSpikeli27
Registered: 12/07/11
Posts: 2
Last seen: 10 years, 11 months
Re: TC Colonization Time? [Re: kdmmontana]
    #15869712 - 02/27/12 09:20 AM (12 years, 10 months ago)

I dont think the strain has anything to do with it. I am growing TC and Burma right now and my qt WBS jars of both strains colonize in about 2 weeks.

I was also worried about TC being slower than the average strain but so far it has been same as the burma as far as myecelium growth. My tubs are colonizing and are not ready for the FC yet.

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Offlinekdmmontana
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Re: TC Colonization Time? [Re: Spikeli27]
    #15869721 - 02/27/12 09:24 AM (12 years, 10 months ago)

I never mentioned strains:P

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OfflineSpikeli27
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Re: TC Colonization Time? [Re: kdmmontana]
    #15869832 - 02/27/12 09:58 AM (12 years, 10 months ago)

Quote:

kdmmontana said:
I never mentioned strains:P




I was replying the OP's question, he asked about the slow growth on his TC jars so I wanted to add that I am also growing TC strain and its not colonizing slower than other strains

Edited by Spikeli27 (02/27/12 10:00 AM)

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OfflineHybridprX
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Re: TC Colonization Time? [Re: Spikeli27]
    #15869899 - 02/27/12 10:12 AM (12 years, 10 months ago)

Sounds like your vendor dug the spores out from a vault and made a syringe.

Two weeks is way to long for germination with "Fresh" spores, but not un-common with older spores. And no, the fact that you stored the syringe for two months prior has nothing to do with it. If anything, leaving the spores in the syringes to "hydrate" will ensure better germination times.


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Offlinekdmmontana
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Re: TC Colonization Time? [Re: HybridprX]
    #15869936 - 02/27/12 10:19 AM (12 years, 10 months ago)

Quote:

HybridprX said:
Sounds like your vendor dug the spores out from a vault and made a syringe.

Two weeks is way to long for germination with "Fresh" spores, but not un-common with older spores. And no, the fact that you stored the syringe for two months prior has nothing to do with it. If anything, leaving the spores in the syringes to "hydrate" will ensure better germination times.




EDIT: wrong thread.

I bet my pension its his subtrate, post up pics so we can see and I bet its too wet.

Edited by kdmmontana (02/27/12 10:25 AM)

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InvisibleShroomerInTheRye
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Registered: 01/12/12
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Re: TC Colonization Time? [Re: kdmmontana]
    #15871907 - 02/27/12 05:45 PM (12 years, 10 months ago)

Okay...here's the best I could do with this crappy cell phone.  I hope this helps.  The first pic is of one of the first jars to start, then stall.  The second is a jar with no growth.  The third is the one in question where it started along the verm line.



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OfflineSwwert
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Re: TC Colonization Time? [Re: ShroomerInTheRye]
    #15871917 - 02/27/12 05:48 PM (12 years, 10 months ago)

looks good to me


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"Maria Sabina is a symbol of wisdom and love."

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InvisibleShroomerInTheRye
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I'm a teapot User Gallery


Registered: 01/12/12
Posts: 13,038
Loc: Themyscira Flag
Re: TC Colonization Time? [Re: HybridprX]
    #15871938 - 02/27/12 05:52 PM (12 years, 10 months ago)

Quote:

HybridprX said:
Sounds like your vendor dug the spores out from a vault and made a syringe.





I got the spores from the most reputable vendor here on the forums and probably the business, so I don't think it's that at all.  See original post for who the vendor was.


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OfflineAmanita virosa
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Re: TC Colonization Time? [Re: ShroomerInTheRye]
    #15872826 - 02/27/12 08:28 PM (12 years, 10 months ago)

yea, thats some slowwwwwwwwwww growth for two weeks.  Really hard to tell  if sub is  too wet from those pix.  def doesnt look too dry though. could just be one of those cube mysteries. IF  it were me,  though  you can bet I would be cracking one open and checking out the insides;  i mean shit ya got twelve...


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