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OfflineDurban.Poison
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Brf hasn't germinated
    #15858643 - 02/24/12 05:51 PM (12 years, 10 months ago)

I am a noob, with a couple of successful brf grows under my belt. I have previously bought pre-sterilized substrate jars, but for my last grow, I decided it was time to make my own jars. I used Bob's Red Mill brf, Sta-Green Horticulture Vermiculite. I followed RR Tek and inoculated with Koh Samui ms that I ordered from a sponsored vendor. It has been 12 days and no signs of germination/colonization.
1) One issue I did have is that I sterilized for 2hr15min, due to the fact that I fell asleep. Could this have been where I went wrong.
2) The syringe I received was totally clear, unlike all the others I have received in the past, which either had clumps or charcoal in them.

Any feedback will be greatly appreciated

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InvisibleKing of Pain
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Re: Brf hasn't germinated [Re: Durban.Poison]
    #15858682 - 02/24/12 06:01 PM (12 years, 10 months ago)

whats the temps like where there stored? is there anything covering your innoc holes?

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OfflineDurban.Poison
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Re: Brf hasn't germinated [Re: King of Pain]
    #15858709 - 02/24/12 06:07 PM (12 years, 10 months ago)

I have them in a double tub incubator that holds between 74 and 80F...there are four inoculation holes that are not covered

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InvisibleKing of Pain
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Re: Brf hasn't germinated [Re: Durban.Poison]
    #15858795 - 02/24/12 06:29 PM (12 years, 10 months ago)

well damn sounds like you know what your doing. Im stumped, could be a bunk syringe. Maybe that strain takes a long time to start.

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OfflineAmanita virosa
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Re: Brf hasn't germinated [Re: King of Pain]
    #15858935 - 02/24/12 07:20 PM (12 years, 10 months ago)

How deep is your verm layer?  are you sure you got the syringe below the verm?  the spores will not grow in the verm layer.  It is also possible  that over sterlizing dried out your substrate too much making it inhospitable to the spores.  Did you let the substrate cool properly before you woke up and shot the spores?  usually, if a syringe is bad, its because it is contaminated, not because its void of spores,but it is possible that you got a syringe with nonviable spores or lacking spores.

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OfflineDurban.Poison
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Re: Brf hasn't germinated [Re: Amanita virosa]
    #15859450 - 02/24/12 09:16 PM (12 years, 10 months ago)

the verm layer was 1/2 in. to 3/8 in. and I am sure I inoculated below this layer. I let it cool for about 27 hours before i used...I left them in the pot without removing lid until I was ready to inoculate...I'm guessing maybe I did dry them out, or maybe the verm I am using is very fine and I should have used more water in the mix. Should the brf mix be to field capacity?

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OfflineShaddox
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Re: Brf hasn't germinated [Re: Durban.Poison]
    #15859470 - 02/24/12 09:20 PM (12 years, 10 months ago)

Being new to it as well i am in the same boat. no growth after 7 days... so i started over on the whole process tonight. gotta keep trying.  best of luck on your grow, mate. hope you get some signs of life.

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OfflineAmanita virosa
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Re: Brf hasn't germinated [Re: Shaddox]
    #15859601 - 02/24/12 09:41 PM (12 years, 10 months ago)

fine verm is good, but i suspect you cooked the water out of the substrate.  shaddox, give it two weeks before giving up on those jars

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OfflineSwwert
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Re: Brf hasn't germinated [Re: Amanita virosa]
    #15859631 - 02/24/12 09:46 PM (12 years, 10 months ago)

Quote:

Amanita virosa said:
fine verm is good, but i suspect you cooked the water out of the substrate.  shaddox, give it two weeks before giving up on those jars



:whathesaid:

PCing so long probably screwed your substrate moisture


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"The mushrooms are just loving you back."
"Maria Sabina is a symbol of wisdom and love."

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OfflineShaddox
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Re: Brf hasn't germinated [Re: Amanita virosa]
    #15859718 - 02/24/12 10:05 PM (12 years, 10 months ago)

Quote:

Amanita virosa said:
shaddox, give it two weeks before giving up on those jars




Oh, I have not given up on them yet. but i did order some more spore syringes from a sponsored vendor and went ahead and prep'd some new BRK cakes for when they arrive. Should be in tomorrow.


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You better take care of me Lord, if you don't you're gonna have me on your hands.

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Offlinesilentshawn
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Re: Brf hasn't germinated [Re: Durban.Poison]
    #15859804 - 02/24/12 10:24 PM (12 years, 10 months ago)

I would say that you most likely dried out the substrate if it was in the P.C. that long.:confused:


--------------------
The Cosmos is also within us, We're made of star stuff.
We are a way for the Cosmos to know itself. -Carl Sagan

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OfflineDurban.Poison
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Re: Brf hasn't germinated [Re: silentshawn]
    #15860891 - 02/25/12 07:50 AM (12 years, 10 months ago)

I actually steam sterilized...not pressure cooked...does that make a difference?

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InvisibleKing of Pain
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Re: Brf hasn't germinated [Re: Durban.Poison]
    #15860955 - 02/25/12 08:27 AM (12 years, 10 months ago)

it shouldn't i steam mine.

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OfflineDurban.Poison
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Re: Brf hasn't germinated [Re: King of Pain]
    #15860974 - 02/25/12 08:33 AM (12 years, 10 months ago)

I have seen people before say that they steam sterilize for 2hr, so I didnt think it was that big of a deal that I went over 15min, unless the moisture content wasn't high enough when I put them in the jars, due to the very fine verm...It make sense to me that since the verm has more surface area that it would require more water, but I may be totally off on this...I really need to know if I should add more water next time, or if there is a way to tell if you got it right?

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OfflineAmanita virosa
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Re: Brf hasn't germinated [Re: Durban.Poison]
    #15861006 - 02/25/12 08:46 AM (12 years, 10 months ago)

if you steam  sterilized it and not pcd it, AND you followed the basic pf tek recipe exactly,  you might have gotten a bum syringe.  Have you opened one  of the jars?  Inspected the  injection sites.  Might as well have alook at this point.  this is why if makes sense to test syringes on agar if  you have the means.  All you need is a SAB some agar and some plates.  That way you can "prove" whether or not a culture is what it is or not.

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OfflineDurban.Poison
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Re: Brf hasn't germinated [Re: Amanita virosa]
    #15861031 - 02/25/12 08:54 AM (12 years, 10 months ago)

I really think I did get a bunk syringe, I just want to rule out the possibility that I made a mistake...My mom has an old microscope I was gonna try and get off her for this reason...So I can give it a look before I waste my time...I just need to come up with a reason that I need it...haha

Does anyone have any input on the moisture level of the mix...like should it be field capacity or should I just stick with the recipe

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OfflineAmanita virosa
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Re: Brf hasn't germinated [Re: Durban.Poison]
    #15862801 - 02/25/12 05:56 PM (12 years, 10 months ago)

I would stick with the recipe.  It is close to field capacity, but it depends on the %moisture of the BRF and verm when you started.  theres a little wiggle room in there so if you followed the recipe you must have had a bum syringe.

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OfflineDurban.Poison
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Re: Brf hasn't germinated [Re: Amanita virosa]
    #15863307 - 02/25/12 07:52 PM (12 years, 10 months ago)

Thanks for all yours and others help...it is greatly appreciated....I love this site

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Offlinekdmmontana
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Re: Brf hasn't germinated [Re: Durban.Poison]
    #15865360 - 02/26/12 08:51 AM (12 years, 10 months ago)

Quote:

Durban.Poison said:
I have them in a double tub incubator that holds between 74 and 80F...there are four inoculation holes that are not covered




You dont need an incubator so you can take them out right now.

Quote:

Amanita virosa said:
fine verm is good, but i suspect you cooked the water out of the substrate.  shaddox, give it two weeks before giving up on those jars




2 weeks is fr to long for germination to take place. I have visible mycelium within 5 days and never had to wait any longer. If his jars dont germinate, I believe it to be because of the substrate blend/water content.

Quote:

Durban.Poison said:
I actually steam sterilized...not pressure cooked...does that make a difference?




Steam sterilizing is fine. You dont need to over do it. I cook my BRF substrates for about 1 hour and thats just fine. Boiling them for too long can cause them to become dry.  Also take care to cover your jars with foil properly.

Post up some pictures of your jars, I can bet my pension on that its the substrate.

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OfflineDurban.Poison
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Re: Brf hasn't germinated [Re: kdmmontana]
    #15865537 - 02/26/12 10:01 AM (12 years, 10 months ago)

I'll get back with those pics...asap...my camera is broke so I will have to borrow one from my buddy...but looking at them, there is an obvious color difference in the mix versus the dry verm layer and what looks to me to be an obvious moisture level difference as well, but I would love to see what you think

As for the incubator...my house stays at bout 40F to 60F this time of year, unless your 2 feet from the Dearborn...I  live in a old drafty house with no insulation...pretty sure the incubator is a must have

thank a bunch

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Shop: Left Coast Kratom Buy Kratom Capsules   Mushroom-Hut Liquid Cultures   PhytoExtractum Kratom Powder for Sale   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate   MagicBag.co Certified Organic All-In-One Grow Bags   Myyco.com Isolated Cubensis Liquid Culture For Sale   Kraken Kratom Red Vein Kratom


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