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PanCyanCambodia@Agar
    #15857379 - 02/24/12 12:12 PM (12 years, 10 months ago)

Hi guys,

i spent a lot of hours on shroomery and google, looking for pictures and postings with the same question, but i'd like to be 100% sure.

the question:
how does Panaeolus-Cyanescens(Cambodia)-Mycelium really looks like?

I'd be happy to get an answer of someone who really knows the structure and look of PanCyanMycelium...


Thanks a lot an i wish a nice Weekend
mirrored.











(
CobwebMold looks to me akin to the mycelium on the pictures but i found people being sad trashing the plates with PanCyan too early...

Workprogress:
With Glovebox, 7 PetriDishes, 1 SporeSyringe, Result: nothing on 6 PetriDishes, no Contamns or Changes, just 1 PetriDish(pictures) after 3 days Spores germinated on 3 points.

It was not the first time i've worked under sterile procedure.

I don't see this as "contamn-question" please move post if it's on the wrong place.
)

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Re: PanCyanCambodia@Agar [Re: mirrored]
    #15857421 - 02/24/12 12:25 PM (12 years, 10 months ago)

looks right buddy.. :super:


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Re: PanCyanCambodia@Agar [Re: evildee125]
    #15858054 - 02/24/12 03:21 PM (12 years, 10 months ago)

yay. thanks! which part would you transfer to the next petri? :-))

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Re: PanCyanCambodia@Agar [Re: mirrored]
    #15858072 - 02/24/12 03:24 PM (12 years, 10 months ago)

2 o'clock


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Re: PanCyanCambodia@Agar [Re: Wing]
    #15861223 - 02/25/12 10:03 AM (12 years, 10 months ago)

okay, thank you guys, i'll update in a few days and transfer 2 o'clock this weekend. can you explain your choice?
thx :-))

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Re: PanCyanCambodia@Agar [Re: mirrored]
    #15861286 - 02/25/12 10:28 AM (12 years, 10 months ago)

It's thick there, but unlike the bottom (3 o'clock - 8 o'clock) it look less cluttered with multiple strains.



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Re: PanCyanCambodia@Agar [Re: mirrored]
    #15861293 - 02/25/12 10:32 AM (12 years, 10 months ago)

2nd picture is kinda awesome

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Re: PanCyanCambodia@Agar [Re: Viridian]
    #15861382 - 02/25/12 11:05 AM (12 years, 10 months ago)

Quote:

Viridian said:
2nd picture is kinda awesome



Seconded.

Does anyone have any experience getting a fruiting strain from cyans on agar? I've been reading its very hard to do. I'm trying to get some accounts before I try.

Not trying to thread jack but it seemed relevant.


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Re: PanCyanCambodia@Agar [Re: Psilocyentist]
    #15862282 - 02/25/12 03:23 PM (12 years, 10 months ago)

i didnt have any problems obtaining a fruiting strain... then again, the majority of agar work i did with panaelous started with mushroom tissue instead of spore


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Re: PanCyanCambodia@Agar [Re: evildee125]
    #15862333 - 02/25/12 03:39 PM (12 years, 10 months ago)

Quote:

evildee125 said:
i didnt have any problems obtaining a fruiting strain... then again, the majority of agar work i did with panaelous started with mushroom tissue instead of spore



Yes I meant from spore.


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Re: PanCyanCambodia@Agar [Re: Psilocyentist]
    #15862457 - 02/25/12 04:11 PM (12 years, 10 months ago)

yeah i know man.. this an instance where my phrasing causes the message to get lost along its mighty way... im really just saying, because of probably the same info youve read, i started off with tissue... i cant remember how from spore went, was so long ago... i'd say go for it.... it seems many of the things  "they" said were hard/toohard, were not that hard at all.. ya feelz meh.. :peace::heart:


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Re: PanCyanCambodia@Agar [Re: evildee125]
    #15865601 - 02/26/12 10:27 AM (12 years, 10 months ago)

Quote:

evildee125 said:
yeah i know man.. this an instance where my phrasing causes the message to get lost along its mighty way... im really just saying, because of probably the same info youve read, i started off with tissue... i cant remember how from spore went, was so long ago... i'd say go for it.... it seems many of the things  "they" said were hard/toohard, were not that hard at all.. ya feelz meh.. :peace::heart:



Alright, I have enough spores to do both so why not?:awehigh:


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Re: PanCyanCambodia@Agar [Re: mirrored]
    #15869296 - 02/27/12 06:32 AM (12 years, 10 months ago)

it's me again, thanks for all your advices. yesterday i made a final picture of the petridish



would you still recommend to choose 2 o'clock? the white part at 1 o'clock seems interesting or am i wrong?

have a nice week!
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Re: PanCyanCambodia@Agar [Re: mirrored]
    #15874769 - 02/28/12 05:14 AM (12 years, 10 months ago)

i don't wanna push this, but it would be nice to get an answer for the next step...

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Re: PanCyanCambodia@Agar [Re: mirrored]
    #15874789 - 02/28/12 05:24 AM (12 years, 10 months ago)

In making choices for picking out petri, your looking for rhizomorphic parts. 

The problem here is your growth is primarily not. SO look up rhizomorphic pancyancambodia on google, and cut as close as possible to match.

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Re: PanCyanCambodia@Agar [Re: cmmenke]
    #15874823 - 02/28/12 05:48 AM (12 years, 10 months ago)

thanks for your answer — i spent plenty of hours in internet (google and shroomery) searching for rhizomorph cyanescens pics, didn't found anything. so i decided to ask the people here before i do something wrong.

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Re: PanCyanCambodia@Agar [Re: mirrored]
    #15874835 - 02/28/12 06:00 AM (12 years, 10 months ago)

dont over think it dear friend.. often, fast growing-rhizomorphic sectoring is sought after.... i myself like to isolate sectors for various reasons... because its thicker or wispier, more rhizomorphic, faster odd looking.. whatever i feel like giving a run to...

i wouldve done the isolating at the time of the first picture you posted,,,

2 o clock is still looking good get that shit already yo..


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Re: PanCyanCambodia@Agar [Re: evildee125]
    #15875113 - 02/28/12 08:19 AM (12 years, 10 months ago)

:whathesaid:

2 o'clock is still my opinion.


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Re: PanCyanCambodia@Agar [Re: mirrored]
    #15877939 - 02/28/12 07:50 PM (12 years, 10 months ago)

mission accomplished. hope that works - transfered four parts of 2 o'clock to new petris. thanks for helping me — speak soon with new results.

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Re: PanCyanCambodia@Agar [Re: mirrored]
    #15903790 - 03/05/12 01:44 PM (12 years, 10 months ago)

hi guys, hope you're all well and i hope to get new inputs and advices. after 5 days, thats the result of the 2 o'clock-mycelium-isolation.



->Petri you already know (2 o'clock) — NOW after transfer




-> transfered mycelium




thoughts? what does look interesting for you? thanks for everything,
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Re: PanCyanCambodia@Agar [Re: mirrored]
    #15912988 - 03/07/12 08:10 AM (12 years, 10 months ago)

i think it's time to transfer, don't wanna push this, but i hope to get some good advices/areas for transfer soon :-)

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Re: PanCyanCambodia@Agar [Re: mirrored]
    #15918930 - 03/08/12 10:57 AM (12 years, 10 months ago)

I personally like the middle pic around 7-8 o'clock


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Re: PanCyanCambodia@Agar [Re: Wing]
    #15923689 - 03/09/12 09:32 AM (12 years, 10 months ago)

okay, thanks for reply!
how long does this process take to transfer the mycelium to rye? i would like to colonize a bag with manure etc.
some inputs?
thanks!! :-) and again...WEEKEND!

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Re: PanCyanCambodia@Agar [Re: mirrored]
    #15925823 - 03/09/12 06:01 PM (12 years, 10 months ago)

It'll usually take 4-6 transfers to get an isolate. Then you might want to test the isolated strain on a small scale to make sure it's a fruiting strain before you do a lot.

Once you have an iso it should colonize quickly on grains or whatever you're using if you have good, healthy, fast mycelium. I'm not sure how fast because it really depends on the strain :shrug:


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Re: PanCyanCambodia@Agar [Re: Wing]
    #15934153 - 03/11/12 04:57 PM (12 years, 10 months ago)

hi guys, first of all: i wish you a nice upcoming week! :-))

secondly i made some new pictures and gave the mycelium names…

Zyklop:


Stimpy:


MeepMeep:


Falcon:


i think the winner is "meepmeep" and second placed "zyklop". i'll transfer good parts to new plates (9 plates left) and if i'm satisfied with some plates, i'll make LC to incubate some bags like in this thread:

Ausi Panaeolus Cyanescens - Liquid Culture to Fruits



:sun: what do you think? advices? :sun:

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Re: PanCyanCambodia@Agar [Re: mirrored]
    #15934337 - 03/11/12 05:45 PM (12 years, 10 months ago)

Great pix. I love zyKlop


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Re: PanCyanCambodia@Agar [Re: Gretchenmeister]
    #15934728 - 03/11/12 07:33 PM (12 years, 10 months ago)

wow awesome pics!! Stimpy all the way :smile:



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Re: PanCyanCambodia@Agar [Re: keeno]
    #15936690 - 03/12/12 08:19 AM (12 years, 10 months ago)

:fonz:


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