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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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slushie9090

Registered: 08/14/01
Posts: 2,000
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RR's video question (inoculating) *DELETED*
#15837908 - 02/20/12 02:27 PM (12 years, 10 months ago) |
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Post deleted by RinReason for deletion: Need to
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LeopardMan
Constantly changing



Registered: 09/21/09
Posts: 5,467
Loc: A tree house
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Re: RR's video question (inoculating) [Re: slushie9090]
#15837983 - 02/20/12 02:42 PM (12 years, 10 months ago) |
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Quote:
Rin said: Unless maybe the tape was to keep water out of the substrate during steam sterilization?
Follow the tek to a T and you won't be disappointed. Good luck
--------------------
You have to die a few times before you can really live.
-Charles Bukowski-
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herfy
Stranger


Registered: 01/26/12
Posts: 111
Last seen: 12 years, 6 months
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Re: RR's video question (inoculating) [Re: slushie9090]
#15837993 - 02/20/12 02:45 PM (12 years, 10 months ago) |
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good observation.But i think the needles circumference is so small that the puncture point is so minute,so nothing to really worry about? good question tho
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crimsondrac
Stranger
Registered: 12/09/11
Posts: 493
Last seen: 11 years, 7 months
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Re: RR's video question (inoculating) [Re: slushie9090]
#15838000 - 02/20/12 02:45 PM (12 years, 10 months ago) |
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The tape is for a tiny bit of air exchange. A hole the size of a syringe needle is not going to be a huge factor. The layer of vermiculite is what is supposed to protect your jar from contamination. The foil you put over the lid is to keep out extra moisture during steaming. And after you pull the needle out of the tape, you can rub it a little and the hole will virtually vanish.
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LeopardMan
Constantly changing



Registered: 09/21/09
Posts: 5,467
Loc: A tree house
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Re: RR's video question (inoculating) [Re: crimsondrac]
#15838034 - 02/20/12 02:51 PM (12 years, 10 months ago) |
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The purpose of micropore tape is to keep water out of your jar. Furthermore it prevents contaminants from landing on your verm layer (so you won't push them into the sub when inoculating).
--------------------
You have to die a few times before you can really live.
-Charles Bukowski-
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crimsondrac
Stranger
Registered: 12/09/11
Posts: 493
Last seen: 11 years, 7 months
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Re: RR's video question (inoculating) [Re: LeopardMan]
#15838096 - 02/20/12 03:01 PM (12 years, 10 months ago) |
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Quote:
LeopardMan said:
The purpose of micropore tape is to keep water out of your jar. Furthermore it prevents contaminants from landing on your verm layer (so you won't push them into the sub when inoculating).
How is a porous tape supposed to keep water out? That is like using a sieve to collect water.
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LeopardMan
Constantly changing



Registered: 09/21/09
Posts: 5,467
Loc: A tree house
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Re: RR's video question (inoculating) [Re: crimsondrac]
#15838115 - 02/20/12 03:05 PM (12 years, 10 months ago) |
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Quote:
crimsondrac said:
Quote:
LeopardMan said:
The purpose of micropore tape is to keep water out of your jar. Furthermore it prevents contaminants from landing on your verm layer (so you won't push them into the sub when inoculating).
How is a porous tape supposed to keep water out? That is like using a sieve to collect water.
This tells me that you never used micropore tape before. It works. That's why everyone uses it.
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You have to die a few times before you can really live.
-Charles Bukowski-
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slushie9090

Registered: 08/14/01
Posts: 2,000
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Re: RR's video question (inoculating) *DELETED* [Re: LeopardMan]
#15838132 - 02/20/12 03:09 PM (12 years, 10 months ago) |
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Post deleted by Rin
Reason for deletion: .
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khani_homesh
Stranger



Registered: 09/24/10
Posts: 77
Loc: Botswana
Last seen: 12 years, 3 months
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Re: RR's video question (inoculating) [Re: slushie9090]
#15838193 - 02/20/12 03:20 PM (12 years, 10 months ago) |
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It's not porous enough that the water will drip through, since water has such high viscosity - and you don't want your moisture level getting jacked up after you worked so carefully to get it right. If you are very concerned about contams after injecting your jars with a hole remaining in the tape, just put another strip over it. The tape is cheap, no reason to go so light. http://www.amazon.com/3M-Micropore-White-Surgical-Tape/dp/B0009I68FE/ref=sr_1_1?ie=UTF8&qid=1329776250&sr=8-1
-------------------- "Do or do not. There is no try." - Yoda
"There are known knowns; there are things we know we know. We also know there are known unknowns; that is to say we know there are some things we do not know. But there are also unknown unknowns – the ones we don't know we don't know." - Rumsfeld
"Every fight is a food fight when you're a cannibal." - Demetri Martin
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LeopardMan
Constantly changing



Registered: 09/21/09
Posts: 5,467
Loc: A tree house
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Re: RR's video question (inoculating) [Re: khani_homesh]
#15838256 - 02/20/12 03:32 PM (12 years, 10 months ago) |
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Quote:
khani_homesh said: It's not porous enough that the water will drip through, since water has such high viscosity - and you don't want your moisture level getting jacked up after you worked so carefully to get it right. If you are very concerned about contams after injecting your jars with a hole remaining in the tape, just put another strip over it. The tape is cheap, no reason to go so light. http://www.amazon.com/3M-Micropore-White-Surgical-Tape/dp/B0009I68FE/ref=sr_1_1?ie=UTF8&qid=1329776250&sr=8-1
No need to add more tape. There is a layer of dry verm specifically designed to protect your substrate from contaminants. If you add another strip to it, you also decrease GE.
--------------------
You have to die a few times before you can really live.
-Charles Bukowski-
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crimsondrac
Stranger
Registered: 12/09/11
Posts: 493
Last seen: 11 years, 7 months
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Re: RR's video question (inoculating) [Re: LeopardMan]
#15838303 - 02/20/12 03:43 PM (12 years, 10 months ago) |
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Quote:
LeopardMan said:
Quote:
crimsondrac said:
Quote:
LeopardMan said:
The purpose of micropore tape is to keep water out of your jar. Furthermore it prevents contaminants from landing on your verm layer (so you won't push them into the sub when inoculating).
How is a porous tape supposed to keep water out? That is like using a sieve to collect water.
This tells me that you never used micropore tape before. It works. That's why everyone uses it.
Oh, I use it all the time, in fact it is the only thing I use. And everytime after I steam or pressure cook, the tape is wet because it absorbs water and can allow it to pass through. It is a breathable tape and allows air and moisture to flow through. Medically, this helps keep a wound dry enough to heal but not so dry that it sticks to the scab. It may keep a majority of the water out as opposed to nothing there at all, but it does allow moisture through.
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khani_homesh
Stranger



Registered: 09/24/10
Posts: 77
Loc: Botswana
Last seen: 12 years, 3 months
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Re: RR's video question (inoculating) [Re: crimsondrac]
#15838343 - 02/20/12 03:53 PM (12 years, 10 months ago) |
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For your future operations, tyvek is free at your local post office, just ask for a few tyvek envelopes. They won't ask questions. Tyvek will allow FAE, but remember the foil to keep tyvek from getting wet. Also, epoxy silicone is decent for ports until you get some actual injection ports.
-------------------- "Do or do not. There is no try." - Yoda
"There are known knowns; there are things we know we know. We also know there are known unknowns; that is to say we know there are some things we do not know. But there are also unknown unknowns – the ones we don't know we don't know." - Rumsfeld
"Every fight is a food fight when you're a cannibal." - Demetri Martin
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slushie9090

Registered: 08/14/01
Posts: 2,000
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Re: RR's video question (inoculating) *DELETED* [Re: khani_homesh]
#15838546 - 02/20/12 04:46 PM (12 years, 10 months ago) |
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Post deleted by RinReason for deletion: ?
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Cynosure
allow me to be your guide.


Registered: 10/06/09
Posts: 4,228
Last seen: 1 year, 10 months
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Re: RR's video question (inoculating) [Re: slushie9090]
#15838590 - 02/20/12 04:55 PM (12 years, 10 months ago) |
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Quote:
Rin said: Thanks to everyone for your help. I'm just going to inoculate through the tape. I don't think it'll be a problem.
It won't be a problem, I promise! I've done it over 50 times with no failures.
-------------------- "You can peel it [language] off the ceiling and make it dance in front of you" - McKenna
<3 .
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HazeyRoms
The Pro Sonn



Registered: 07/19/08
Posts: 542
Loc: Wisconsin
Last seen: 11 years, 7 months
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Re: RR's video question (inoculating) [Re: Cynosure]
#15838706 - 02/20/12 05:19 PM (12 years, 10 months ago) |
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RR is an animal. I know this has nothing to do with the question, I just had to give him thumbs up on everything he does.
-------------------- -Sonn, Hazey here. And I'm bacc. Anyone in Wisconsin, PM me. Unity my nillas/niggas.
-"5 Nike duffle bags??? How much does all that weigh?? - Just 30 pounds Bruhh, relax my nilla, it don't stink like weed so we're all good."-(Penis Envy Moments)-
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herfy
Stranger


Registered: 01/26/12
Posts: 111
Last seen: 12 years, 6 months
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Re: RR's video question (inoculating) [Re: HazeyRoms]
#15839016 - 02/20/12 06:10 PM (12 years, 10 months ago) |
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Quote:
HazeyRoms said: RR is an animal. I know this has nothing to do with the question, I just had to give him thumbs up on everything he does.
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 10 months
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Re: RR's video question (inoculating) [Re: crimsondrac]
#15839078 - 02/20/12 06:20 PM (12 years, 10 months ago) |
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Quote:
crimsondrac said: Oh, I use it all the time, in fact it is the only thing I use. And everytime after I steam or pressure cook, the tape is wet because it absorbs water and can allow it to pass through. It is a breathable tape and allows air and moisture to flow through. Medically, this helps keep a wound dry enough to heal but not so dry that it sticks to the scab. It may keep a majority of the water out as opposed to nothing there at all, but it does allow moisture through.
You've missed the point entirely. The foil over the jars keeps the water out during sterilization. The tape is an extra barrier(in addition to the dry verm) to help block contaminant spores as the jars cool down and compact. This compaction during cooling causes air to be drawn into the jar as the substrate shrinks from cooling. Once the jars are cool and you inoculate, you no longer need that extra barrier, so it's OK to puncture it. RR
-------------------- Download Let's Grow Mushrooms
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"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
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slushie9090

Registered: 08/14/01
Posts: 2,000
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Re: RR's video question (inoculating) *DELETED* [Re: RogerRabbit]
#15839289 - 02/20/12 06:59 PM (12 years, 10 months ago) |
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Post deleted by Rin
Reason for deletion: .
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LeopardMan
Constantly changing



Registered: 09/21/09
Posts: 5,467
Loc: A tree house
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Re: RR's video question (inoculating) [Re: slushie9090]
#15840961 - 02/21/12 01:41 AM (12 years, 10 months ago) |
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Micropore tape helps keeping the water out during sterilization. Foil is essential, MT is very useful. RR said that many times before and experience confirmed it. Let's not over complicate things.
--------------------
You have to die a few times before you can really live.
-Charles Bukowski-
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crimsondrac
Stranger
Registered: 12/09/11
Posts: 493
Last seen: 11 years, 7 months
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Re: RR's video question (inoculating) [Re: RogerRabbit]
#15890194 - 03/02/12 12:19 PM (12 years, 10 months ago) |
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Quote:
RogerRabbit said:
Quote:
crimsondrac said: Oh, I use it all the time, in fact it is the only thing I use. And everytime after I steam or pressure cook, the tape is wet because it absorbs water and can allow it to pass through. It is a breathable tape and allows air and moisture to flow through. Medically, this helps keep a wound dry enough to heal but not so dry that it sticks to the scab. It may keep a majority of the water out as opposed to nothing there at all, but it does allow moisture through.
You've missed the point entirely. The foil over the jars keeps the water out during sterilization. The tape is an extra barrier(in addition to the dry verm) to help block contaminant spores as the jars cool down and compact. This compaction during cooling causes air to be drawn into the jar as the substrate shrinks from cooling. Once the jars are cool and you inoculate, you no longer need that extra barrier, so it's OK to puncture it. RR
Roger, with all due respect, you quoted me out of context. I am on your side. I know Foil is to keep water out, I know all the tape for is to help with GE. I was arguing the next post (post 5) where LeopardMan was saying that the tape was to keep moisture out(with some stupid Rolling Eyes smiley) and I said how does porous tape keep moisture out? Then all you read was my reply, out of context, and decided my statement was wrong. Micropore tape IS NOT a moisture barrier.
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