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InvisibleVersicolor
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Create a sporeless strain by isolating one spore?
    #15816084 - 02/15/12 09:54 PM (12 years, 3 days ago)

I was thinking. What would happen if you germinated and isolated a single spore of say P.ostreatus?
I would think it would grow into a culture that could colonize and create mushrooms, but lack the sexual genotype to create spores.
Is that how a sporeless strain would be created?
Just not allow a sexual encounter, therefore removing the necessary genome to reproduce?
The only way to multiply a strain like this would be a tissue sample(no spores), therefore genetic fading would eventually prevent it from growing.
I can't find any information regarding this idea.
I've heard that when doing a multi-spore inoculation to agar, to let the spores mate with other compatible colonies before sectoring.
Any thoughts would be appreciated.


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OfflineAmanita virosa
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Re: Create a sporeless strain by isolating one spore? [Re: Versicolor]
    #15816126 - 02/15/12 10:02 PM (12 years, 3 days ago)

the mushroom is the sexual reproductive organ of a fungus.  Ascomycetes and basidiomycetes will not make fruiting bodies (what we eat) without first forming dikaryotic hyphae; in order to get a dikaryotic hyphae, you gotta have two spores and they must be sexually compatable.  One spore gives you endless mycelium searching for a mate and never making a mushroom.  As to their sexual orientation, they are not boy and girl like animals but mating strains;  some species as simple as Plus and Minus;  others like  shitake are more complicated in terms of which spores are compatible with others.  Hope this explains it


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Re: Create a sporeless strain by isolating one spore? [Re: Amanita virosa]
    #15816173 - 02/15/12 10:15 PM (12 years, 3 days ago)

That occurred to me that it wouldn't produce hyphae.
I thought there was a chance that it would be able to fruit, just not create basidia or spores on the gill plane.
Any idea on how Paul Stamets was able to create a sporeless fruitbody?


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OfflineRogerRabbitM
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Re: Create a sporeless strain by isolating one spore? [Re: Versicolor]
    #15816202 - 02/15/12 10:22 PM (12 years, 3 days ago)

You don't 'create' a sporeless strain except by accident.  Usually, one just pops up now and then, and if it has the other characteristics you want, you clone and save it.  It's fairly common around here for people to 'complain' of sporeless cubes, when instead they should clone it and be thankful.
RR


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Re: Create a sporeless strain by isolating one spore? [Re: RogerRabbit]
    #15816234 - 02/15/12 10:27 PM (12 years, 3 days ago)

Ok, good to know.
He publishes his 'sporeless' mushrooms then doesn't explain. Just curios.
Thanks


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Re: Create a sporeless strain by isolating one spore? [Re: RogerRabbit]
    #15816452 - 02/15/12 11:19 PM (12 years, 3 days ago)

Quote:

RogerRabbit said:
It's fairly common around here for people to 'complain' of sporeless cubes, when instead they should clone it and be thankful.
RR



:rockon:


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OfflineAmanita virosa
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Re: Create a sporeless strain by isolating one spore? [Re: total]
    #15817337 - 02/16/12 06:09 AM (12 years, 3 days ago)

Quote:

total said:
Quote:

RogerRabbit said:
It's fairly common around here for people to 'complain' of sporeless cubes, when instead they should clone it and be thankful.
RR



Yea, you'd think people were snorting up big fat lines of them!


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Re: Create a sporeless strain by isolating one spore? [Re: Amanita virosa]
    #15819970 - 02/16/12 06:15 PM (12 years, 2 days ago)

lmao, I found a sporeless cube strain, cloned that sucker right away. it was a small cluster of sporeless cubes lol


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