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InvisibleJavadog
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Re: Lepista nuda [Re: atricoz]
    #15868138 - 02/27/12 12:03 AM (8 years, 7 months ago)

Nice stuff atricoz!

I have high hopes for your grow.

Thanks for the update.

JD


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Re: Lepista nuda [Re: Javadog]
    #15869409 - 02/27/12 09:35 AM (8 years, 7 months ago)

Wow, atricoz, your spawn looks extremely healthy and strong!

@metacohl
Here is an old picture of L. nuda mycelium on compost, right after spore germination:

I guess it would appear differently on agar, but you should see slightly curled hyphae, growing more straight after a couple of days.

Carsten


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Re: Lepista nuda [Re: Mycelio]
    #15869472 - 02/27/12 10:03 AM (8 years, 7 months ago)

Quote:

Mycelio said:
@metacohl
Here is an old picture of L. nuda mycelium on compost, right after spore germination:
I guess it would appear differently on agar, but you should see slightly curled hyphae, growing more straight after a couple of days.

Carsten




Thanks Carsten. After reading your post I am now positive that some of the colonies growing on the plate I posted are L. Nuda. Unfortunately, the mold on the plate has obviously begun sporulating. I am not very experienced with agar, but it seems unwise to open a plate of sporulating mold when I still have spores to work with.

It seems that my spore print may be highly contaminated with mold. I was thinking by using the streaking method commonly used for bacteria I may be able to isolate the mold and mushroom spores. However, with the seemingly long germination time for spores of this species I am worried that the mold will begin sporulating again before I have time to identify the colonies of L. Nuda......

Any thoughts?


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Re: Lepista nuda [Re: metacohl]
    #15869563 - 02/27/12 10:40 AM (8 years, 7 months ago)

Yes, starting again and streaking sounds good. You usually have two or three days before molds sporulate, so you can try removing anything that appears too early or looks odd. Just lift the lid at one side as much as you need to get your tool in and out, to keep the contamination risk as low as possible.

Carsten


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Re: Lepista nuda [Re: Mycelio]
    #15883298 - 02/29/12 11:35 PM (8 years, 7 months ago)

Hello all,

I wanted to check in.

The spawn is colonized (recall, added compost and made it on the dry side):


The orange color concerns me...this is looking like the straight
grain spawn that I failed with on my previous attempts.

Check the latest agar tests.  Here we have WPDWYA, anti-biotic MEA,
and PMEA next to each other:


The PMEA dishes are interesting.  The xfer from the PDYA dish is
still healthy looking, but the inoculant from the MEA dish has darkened.
(that is the fogged up dish.  Sorry!)  Also, the growth on the PMEA
is whitish and not showing the blackening die-back.  ...it has not
grown out far enough to tell if this difference will hold.

I will go ahead and pasteurize a sub to test the spawn out on, though
I have little expectations of success. 

I think that I will make a coir based sub as well as a hpoo compost sub,
just to see what this does.  ...we will see. 

Take care,

JD

P.S. I am getting my first P. caerulescens pins!



Hoo hoo!


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Edited by Javadog (02/29/12 11:36 PM)


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Re: Lepista nuda [Re: Javadog]
    #15892312 - 03/02/12 10:34 PM (8 years, 7 months ago)

It's incredible how the mycelium looks different on each petri. In the 2 on the left, is seems to have some greenish layers too :ooo:

I have some update, now the substrate looks almost practically completely colonized by a thin, light violet mycelium. I'm wondering if it's the moment to add a casing layer or not :crazy:







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Re: Lepista nuda [Re: atricoz]
    #15892553 - 03/02/12 11:46 PM (8 years, 7 months ago)

Dang...I like your stuff sooo much more than mine.

I made a new sub that I want to try with my L. nuda anyway.

We need to trade a wedge or something....maybe I can go back to the
spore print that I took from the wild found fruit I started with.

I am definitely outside the lines on this one.

Oh, and I cannot be certain, but I would go ahead and add a casing layer.
I would let it be colonized before I put it into fruiting conditions.
This is an interesting phase, as the casing layer will need to be living.
I am not sure that just 50/50 pasteurized will suffice.

If I had ten trays like that I would literally take dirt from my back yard
and pasteurize that for one of them.  The same with plenty of leaves
and twigs for another.  Etc...

Good luck.  I am really happy that you joined this thread.

JD


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Re: Lepista nuda [Re: Javadog]
    #15899086 - 03/04/12 03:05 PM (8 years, 7 months ago)

Well, I went ahead and spawned the dryish-grain-plus-WBS spawn to
two tubs.  I have little expectation of success, but I did get to work
with a new sub element.

I went to our local landfill and got a trashcan of the mulch that is
made from our greenery waste.  It is relatively fine, very dark, and
well broken down.

I used mainly this, but added some hpoo, chopped straw, verm, and gypsum.



(I am thinking that RR might get a laugh from my repurposing of
otherwise useless materials)

Take care,

JD


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Re: Lepista nuda [Re: Javadog]
    #15899832 - 03/04/12 06:13 PM (8 years, 7 months ago)

Nice work so far, I am curious to see any growth differences with mine. I'm going to place a casing layer next week, mycelium is still fluffy and didn't clump the substrate :crazy:


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Edited by atricoz (03/04/12 06:14 PM)


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Re: Lepista nuda [Re: atricoz]
    #15900436 - 03/04/12 08:45 PM (8 years, 7 months ago)

Just want to give an update on my L. nuda attempt.
I had 1 sawdust block that was fully colonized, it smelled just like a fresh blewitt. After 3 weeks in the FC my sawdust bag contamed with green mold.
I did not case it, which I think was a big mistake. The myc is so thin that it is prone to drying and some sort of overlay happens.
I'm about to try the strain that pinned on agar, so we'll see.
I'm also going to incorporate hardwood leaves into the next sub.

looking goog atricoz, I think JD is right about casing it.


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Re: Lepista nuda [Re: bambus]
    #15900443 - 03/04/12 08:47 PM (8 years, 7 months ago)

Thanks for sharing Bambus.

I appreciate your joining the effort.  One of us will get fruits, then
we can all try whatever they did.  ;0)

Good luck,

JD


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Re: Lepista nuda [Re: Javadog]
    #15902330 - 03/05/12 06:47 AM (8 years, 7 months ago)

Wow.
I love this species. (particularly with garlic, onion and olive oil)
In january 2011 i did an experiment where i did put cardboard, fallen leaves (oak), pin needles, barks and coffee grounds in a tray.
Then i did add many blewitt stem butts. After some weeks i saw a lot of mycelium walking everywhere. And yes, the colour was exactly the same that we can notice in Atricoz snaps, a pale lilac.
A month later, more or less, i did put a casing layer with pasteurized potting soil and a bit of sand. I wanted to ricreate a similar habitat to that where theses beauties live.
But although the mycelium did cover almost fully the casing layer several days later, i never saw any sign of pin heads so, after some weeks, i did empty the tray in a little wood near my home.
So far i'm still waiting my blewitts...hoping that the mycelium is still alive :rolleyes:
For more details
here you find my log
http://www.veggiegardeningtips.com/blewitt-mushrooms-for-the-adventurous-gardener/

Unfortunately, this last autumn-winter, through lack of rain, i didn't find any Lepista nowhere.:mad2:

A big thanks to all of you, guys. This is a fantastic thread:thumbup:


Edited by ILBIACCO (03/05/12 11:34 AM)


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Re: Lepista nuda [Re: ILBIACCO]
    #15902648 - 03/05/12 10:09 AM (8 years, 7 months ago)

...and thank you very much for sharing.

I only had the one wild find to start with, and I started from spores,
but I do seem to be the odd man out as far as the appearance of the
mycelium goes. 

The starting point:


I appreciate your sharing your sub recipe...that is rather up in the air at this point.
I can only think of the place where I found my fruit.

Good luck!

JD


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Re: Lepista nuda [Re: Javadog]
    #15903233 - 03/05/12 01:22 PM (8 years, 7 months ago)

I am an absolute beginner in mushroom cultivation.
I know zip about agar, honestly.
As you know some species are able to regrow from stem butts:
Pleurotus ostreatus is propably the first and most well-known example. My growing attempts, for now, are limited to this species.
But, if in the future i have another specimen of blewitt between my hands, i will do another try, surely.
Another species that, in my opinion, is ideally suited for my tray of woodchips, fallen leaves, pine needles and so on is Clitocybe Geotropa.
A very tasty mushroom:crazy2: with a big "fluffy" stem butt, and
many pieces of mycelium attached to it.
Good luck to you!


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Edited by ILBIACCO (03/05/12 01:24 PM)


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Re: Lepista nuda [Re: ILBIACCO]
    #15903469 - 03/05/12 02:35 PM (8 years, 7 months ago)

I just found a Blewit patch and took a couple prints. Does anyone know if you can start cultivation with spore syringes and BRF? I dont have a pressure cooker so i can't do WBS, or Wheat berries. Thanks


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Re: Lepista nuda [Re: dirt]
    #15903477 - 03/05/12 02:37 PM (8 years, 7 months ago)

I bet that you can make spawn this way, but you will want to spawn it
to a tub of a better sub for fruiting, I think.

Do it!  ;0)

JD

P.S. A parallel is using BRF cakes to start a Panaelous grow.  It works
fine, but you want to use a manure based sub for fruiting. (I am as I type)


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Re: Lepista nuda [Re: Javadog]
    #15903545 - 03/05/12 02:51 PM (8 years, 7 months ago)

Cool, thanks much JD. I'm planning on knocking up a couple test jars and then 8 jars if all goes well. I'd like to  have plenty to work with so I can experiment with different substrates. I'll keep you posted, but not expecting anything until next year.

Thanks


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Re: Lepista nuda [Re: dirt]
    #15903851 - 03/05/12 03:59 PM (8 years, 7 months ago)

Of course, thank you for jumping in.  I read of one limited success where
a six month colonization period was observed.  Wow.  We will see.

Good luck,

JD


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Re: Lepista nuda [Re: Javadog]
    #15936354 - 03/12/12 06:27 AM (8 years, 7 months ago)

Hey guys,
where have you gone?
Updates, please.:grin:


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Re: Lepista nuda [Re: ILBIACCO]
    #15936503 - 03/12/12 08:36 AM (8 years, 7 months ago)

yesterday I spreaded another layer of compost, and finally cased with 50/50 vermiculite and peat moss. I'll post some pic in the next days :smile:


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Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

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