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InvisibleJavadog
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Lepista Nuda
    #15786545 - 02/10/12 02:25 AM (8 years, 8 months ago)

Hello all,

I have tried a couple of times to get L. nuda to colonize a filter patch
bag with a compost like sub two times and have not gotten good results.
(failed to colonize)

One thing that I have noticed is that on agar it seems to turn black as
it matures.  I put agar on grains and got strong colonization, with the
grains looking like cheetos bits (very orange and colorful).

...but the spawn jars consistently began to look discolored, wet, and
rotten at the bottom of the quart jars. 

It seemed that I had a ride-along bacterial infection going on.

This last pass, I put it onto anti-biotic agar, but I am seeing
similar looking growth:


I made transfers to fresh, non anti-biotic, agar from the clean and
white leading edge, so we will see where that goes.

Has anyone else seen this?

Please chime in with any special needs of L. nuda, and thank you for the time!

JD

P.S. An earlier agar shot, posted months ago:


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Re: Lepista Nuda [Re: Javadog]
    #15786945 - 02/10/12 04:36 AM (8 years, 8 months ago)

Hey JD,

good luck! So far all my experiments ended up rotting.

I think the color changes on Agar depends on strain and nutrients. However, it is very difficult to keep L. nuda mycelium happy. A compost or manure based substrate should be fine, but always take great care that it is on the dry side and enough oxygen is provided. For grain spawn I had better results when mixing in some compost.

Carsten


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Re: Lepista Nuda [Re: Mycelio]
    #15787695 - 02/10/12 10:54 AM (8 years, 8 months ago)

Quote:

Mycelio said:
Hey JD,

good luck! So far all my experiments ended up rotting.

I think the color changes on Agar depends on strain and nutrients. However, it is very difficult to keep L. nuda mycelium happy. A compost or manure based substrate should be fine, but always take great care that it is on the dry side and enough oxygen is provided. For grain spawn I had better results when mixing in some compost.

Carsten




Carsten, you are golden.  I really appreciate knowing that I am not alone!

...it is soooo weird.  My grain spawn looked *perfect*....then a couple
weeks later I was left trying to spawn just the top half grains.

I will be continuing to experiment with this species.  It is a local
wild find and I want to know it better.

I will try the experiments you suggest. 

Thanks again,

JD


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Re: Lepista Nuda [Re: Javadog]
    #15787781 - 02/10/12 11:17 AM (8 years, 8 months ago)

You guys get this figured out and let me know. I found two growing in my yard a few years ago but they never showed up again. I want to start a bed if you will figure it out for me. Thanks for all the hard work.


--------------------
I need tropical cultures, ABM, V.v....!!!!

Well things don't always look as they are and things can be misread and mistaken for what they realy are so don't read too much into what I say since I might be mistaken myself. And remember I rarely ever give a definate answer.


Edited by trout (02/10/12 11:19 AM)


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Re: Lepista Nuda [Re: trout]
    #15787913 - 02/10/12 12:03 PM (8 years, 8 months ago)

Will do.

This is going to take a long time, as I am working with dozens of species
at the same time, as usual....but it will happen.

I even have a spore print to fall back on, and I think that I can because
at this point a non-fruiting strain is no problem.  I am still working
on strong spawn and then strong colonization.

Take care,

JD


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Re: Lepista Nuda [Re: Javadog]
    #15789700 - 02/10/12 08:40 PM (8 years, 8 months ago)

I have 2 strains going right now, one of them is pinning on agar.
I also have 1 fully colonized sawdust/newspaper/h-manure bag that I just put into my FC. This is my first try with them, but my plates have no discoloration, just very fluffy ariel whitish myc.


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Re: Lepista Nuda [Re: bambus]
    #15791441 - 02/11/12 07:29 AM (8 years, 8 months ago)

Wow, did you clone the pinning strain yourself? Can you show a picture?

Carsten


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Re: Lepista Nuda [Re: Mycelio]
    #15791764 - 02/11/12 10:29 AM (8 years, 8 months ago)

Wow indeed.

I had come to think that this species' mycelium was consistently colored.

...and I had thought to work with multiple genetics before isolating strains.
I am more interested in the needs of this species than I am in yield at this point.

Perhaps an isolate would omit the destructive behavior?

Interesting.  Please do post photos! 

Thanks for sharing.

JD


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Re: Lepista Nuda [Re: Javadog]
    #15792002 - 02/11/12 12:05 PM (8 years, 8 months ago)

Quote:

Javadog said:
I am more interested in the needs of this species than I am in yield at this point.




I don't know much about growing, but I always find them where the leaf/needle litter covers some older wood chips.


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Re: Lepista Nuda [Re: Mycelio]
    #15804737 - 02/13/12 09:00 PM (8 years, 8 months ago)

Yes, I cloned 2 strains from wild specimens.
Only one plate has pins on it so far.
I thought this species would need a casing layer, but I guess not.
Here are some pics of the pins (sorry for the poor quality, taking pictures through glass is a challenge).





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Re: Lepista Nuda [Re: bambus]
    #15806040 - 02/14/12 12:40 AM (8 years, 8 months ago)

Wow.  All this white growth...

Thanks for sharing!

JD


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Re: Lepista Nuda [Re: Javadog]
    #15812146 - 02/15/12 07:16 AM (8 years, 8 months ago)

That picture is just amazing. Did you use some unusual agar recipe to induce pins?


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Re: Lepista nuda [Re: atricoz]
    #15812173 - 02/15/12 07:45 AM (8 years, 8 months ago)

I hate to say this and I hope I am wrong, but that pure white mycelium as well as the small fruitbodies appear suspicious to me. Any chance you had oysters fruiting in your flat when working with that culture, bambus?

I have only seen three strains of L. nuda so far, but each time the mycelium wasn't pure white, it always had some purple color.

Carsten


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Re: Lepista nuda [Re: Mycelio]
    #15812906 - 02/15/12 12:35 PM (8 years, 8 months ago)

I must concur.

I find that the growth is white for a day or two, but it darkens as
the growth continues.

This was the result of transferring just a white leading-edge bit from
an anti-biotic agar dish to a fresh regular agar (MEA) dish:


The coloration does not seem to be an infection, but part of the life
cycle of this species.....it may be a negative indication (I need to confirm
or deny this!) of something undesirable going on, but it might also
just be how it grows out.

I am open to anything, as I know zip about this species so far.

I am driven, in part, by the fact that I am working with a wild find
and really think that I will be able to make this work.

Thank you all for taking the time,

JD


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Re: Lepista nuda [Re: Javadog]
    #15813134 - 02/15/12 01:27 PM (8 years, 8 months ago)

I should have been more clear in my first post, I said "whitish".
I meant not pure white, it does have a lavender hue to it, but it is faint.
The well formed pins are sprouting directly from the piece of cloned tissue.
These pins look nothing like the pins on my oyster plates.
They could be pinning due to the temps, my plates are stored at 45F-60F.
The temp fluctuates more than it probably should.
I also have G. frondosa pins on agar believe it or not.


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Re: Lepista nuda [Re: bambus]
    #15813316 - 02/15/12 02:12 PM (8 years, 8 months ago)

Alright, 'not pure white, with a lavender hue' sounds perfect.

JD, I wasn't clear, I meant young aerial mycelium, which should have that color. Browning on MEA seems to be a different effect, which also happens with several strains of Agaricus bisporus. It seems to be coupled with weaker growth, so perhaps there is some nutrient deficiency, causing older mycelium to die, so younger hyphae can continue to grow.
PDYA usually supports strong and fluffy growth, while the secreted enzymes stain the agar almost blue. There is a nice picture here:
http://kulturpilz.de/viewtopic.php?p=17260#p17260

Carsten


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Re: Lepista nuda [Re: Mycelio]
    #15814073 - 02/15/12 05:38 PM (8 years, 8 months ago)

Thanks Carsten.  I will look for this, and will have to try varying
my agar recipe too.  (I was looking to do this already and have potato
dextrose, etc, ready to go)

I did want to reiterate that my genetics are based on a wild find and
are as strong, to the eye, as I would expect from a wild sample.

It has grown strongly on agar and ever stronger on grains.

...it just never stops the transition from purple-orange to orange,
to darker orange, and ending up blackish.  I need to look at one of
these dark areas under the scope, as I have no idea what is happening
in those areas.

Thanks again,

JD


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Re: Lepista nuda [Re: Javadog]
    #15814833 - 02/15/12 08:10 PM (8 years, 8 months ago)

The agar I used was the corn-steep pre-mix from mushroompeople.
I also added a bit of H-manure to it when cloning this species.
The fully colonized grain jars show no "spots" whatsoever. The mycelium has a very uniform color to it. The growth is not rhizomophic at all, just light & fluffy.


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Re: Lepista nuda [Re: bambus]
    #15814894 - 02/15/12 08:17 PM (8 years, 8 months ago)

OK, I am going to make two new sleeves of agar dishes.

One will be potato based and the other will be plain MEA agar plus manure.

Thanks!

JD


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Re: Lepista nuda [Re: Javadog]
    #15815076 - 02/15/12 08:43 PM (8 years, 8 months ago)

Looking forward to updates JD

-Mike


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Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

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