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OfflineKissmegxxdnite
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Registered: 12/15/11
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Sterilizing fresh syringes
    #15767026 - 02/06/12 02:08 AM (12 years, 11 months ago)

Ok I am going to draw up some liquid culture into fresh syringes is it neccessary to flame sterilize prior to it being drawn up. This is being done in a SAB. This lc was made using ottos begginer friendly lc tek prior to me having a PC usually I can only get on syringe from this tek then the jar contams so I would rather store them in a syringe since there's no self healing port only a hole in the lid with foil covering it. I am doing and lme culture with proper lids next this strain colonized super quick. So I want to preserve it. Thank you for your answers in advance.

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OfflineSan
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Registered: 10/30/11
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Re: Sterilizing fresh syringes [Re: Kissmegxxdnite]
    #15767081 - 02/06/12 02:47 AM (12 years, 11 months ago)

Otto's is a multispore LC iirc. Are you just going to switch out the spoors for your isolate?

Make sure to back up your isolate away from your LC in case it goes to shit. In this case I think that means just keeping the original agar plate or making a slant or something.

As for the LC... Make some test jars! Can't go wrong.


--------------------
Actually not everyone was a noob.  Being a noob is a very new phenomenon.  Many people, the great majority in fact, were simply "beginners", "novices" or "new to mushroom growing".  Being a "noob" is reserved, and in fact created specifically for and by, the newer, much more lame generations coming about.

-Shpongle1

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OfflineKissmegxxdnite
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Re: Sterilizing fresh syringes [Re: San]
    #15767123 - 02/06/12 03:30 AM (12 years, 11 months ago)

I have tested it and it's clean. I made 3 at the time 1 contamed the other two tested and worked perfectly but one of those two contaminated shortly after this last one has not contaminated. I drew up 12ccs of the culture into two syringes and re-sealed the jar. I am watching closely to see if it will contaminate. I have made proper lc lids with GE port(the kind that dont get wet) whatmans and self healing port for an lme culture. I am also waiting on a few pints of rye to fully colonize for a glc.

I flamed the syringes to be safe.

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