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Offlinennn-yan
Shaman Norse Warrior
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Registered: 09/15/11
Posts: 376
Last seen: 7 years, 6 days
mycelium and honey/karo
    #15762887 - 02/05/12 01:12 AM (12 years, 11 months ago)

i had a question for all,
after reading the poor man's mycelium syringe tek and the process it got me thinking that instead of isolating genes by potatoe dextrose in petri dishes could i use honey/karo and water and inject it with spore? or use a mycelium syringe?
thanks


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Men can give birth.....:mushroom2:TO MUSHROOMS!!:mushroom2: Largest 140g (wet)

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OfflineHyphaeHunter
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Registered: 09/24/11
Posts: 23
Last seen: 9 years, 5 months
Re: mycelium and honey/karo [Re: nnn-yan]
    #15762939 - 02/05/12 01:30 AM (12 years, 11 months ago)

Sure as long as you follow correct sterile procedures.  Would be a LC if thats what your going for.  I know most teks involve using a PC, but I have been reading up on one lately that was originally posted a while ago that I just found but it is a steam sterilize method.  Seems to work for many members.

www.shroomery.org/forums/showflat.php/Number/5238137/fpart/1/vc/1/nt/25

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OfflineKizzle
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Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 8 days
Trusted Cultivator
Re: mycelium and honey/karo [Re: HyphaeHunter]
    #15762982 - 02/05/12 01:49 AM (12 years, 11 months ago)

I don't think that would really help you isolate a single strain if that's what you mean.


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Offlinennn-yan
Shaman Norse Warrior
Male


Registered: 09/15/11
Posts: 376
Last seen: 7 years, 6 days
Re: mycelium and honey/karo [Re: Kizzle]
    #15762984 - 02/05/12 01:51 AM (12 years, 11 months ago)

no i just meant to get one healthy source of mycelium, to make mycelium syringes for betterf incoulations for the pf tek


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Men can give birth.....:mushroom2:TO MUSHROOMS!!:mushroom2: Largest 140g (wet)

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OfflineKizzle
Misanthrope
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Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 8 days
Trusted Cultivator
Re: mycelium and honey/karo [Re: nnn-yan]
    #15763000 - 02/05/12 02:00 AM (12 years, 11 months ago)

You can inoculate an LC with spores, mycelium water, another LC, mushroom tissue, or a wedge from a mycelium culture. Out of those options spores seems to be the most likely to contaminate.


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