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Cynosure
allow me to be your guide.


Registered: 10/06/09
Posts: 4,228
Last seen: 1 year, 11 months
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Cutting growth from agar;
#15759905 - 02/04/12 11:55 AM (12 years, 11 months ago) |
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Just a quick question:
How do you guys cut your healthy growth from agar for a transplant in tall half pint jars or quart jars?
I just attempted to do so with a scalpel but it was very messy and I'm pretty sure I compromised the jar to contaminants.
-------------------- "You can peel it [language] off the ceiling and make it dance in front of you" - McKenna
<3 .
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cc2
Mush

Registered: 05/15/10
Posts: 2,611
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Re: Cutting growth from agar; [Re: Cynosure]
#15760140 - 02/04/12 12:59 PM (12 years, 11 months ago) |
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Quote:
Cynosure said: Just a quick question:
How do you guys cut your healthy growth from agar for a transplant in tall half pint jars or quart jars?
I just attempted to do so with a scalpel but it was very messy and I'm pretty sure I compromised the jar to contaminants.
what was your issue exactly? you just have to cut a piece, and pry it from the remaining agar. you can also just shave the upper portion since the lower agar is not colonized. even sterilizing a hypodermic needle, cutting with it and transfering is feasible.
btw, having a flowhood when doing transfers is not necessary but helps a lot.
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Cynosure
allow me to be your guide.


Registered: 10/06/09
Posts: 4,228
Last seen: 1 year, 11 months
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Re: Cutting growth from agar; [Re: cc2]
#15760220 - 02/04/12 01:20 PM (12 years, 11 months ago) |
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Well, I cut it out but couldn't get it up and out of the jar. I ended up having to fool around with it for a while in order to press the growth on the side of the jar and drag it up. All of this movement plus the amount of time I had it exposed to air (I don't have a flow hood and it was too hard to do inside of my glove box) makes me think it's going to get badly contaminated.
I have ordered some petri dishes in the meantime (and have some from a member here coming as a trade) but need a better solution for the growth that I have now. I have about 8 half pint jars left to do.
-------------------- "You can peel it [language] off the ceiling and make it dance in front of you" - McKenna
<3 .
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cc2
Mush

Registered: 05/15/10
Posts: 2,611
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Re: Cutting growth from agar; [Re: Cynosure]
#15760253 - 02/04/12 01:26 PM (12 years, 11 months ago) |
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Quote:
Cynosure said: Well, I cut it out but couldn't get it up and out of the jar. I ended up having to fool around with it for a while in order to press the growth on the side of the jar and drag it up. All of this movement plus the amount of time I had it exposed to air (I don't have a flow hood and it was too hard to do inside of my glove box) makes me think it's going to get badly contaminated.
I have ordered some petri dishes in the meantime (and have some from a member here coming as a trade) but need a better solution for the growth that I have now. I have about 8 half pint jars left to do.
I see, I didn't understand you were using tall jars as petri dishes for agar. if you can, use shallow jars. as of now best I can suggest is to either wait for petri dishes or use a needle to cut out, flip over and punch thru a healthy colonized piece.
also work with your jar sideways if you can, dust is always falling down and I think in this way you're a tiny tiny bit more clean than with the opening facing up. don't stir air up, try to work in your glovebox and use smaller jars or something better next time. 
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Cynosure
allow me to be your guide.


Registered: 10/06/09
Posts: 4,228
Last seen: 1 year, 11 months
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Re: Cutting growth from agar; [Re: cc2]
#15760432 - 02/04/12 02:03 PM (12 years, 11 months ago) |
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All solid advice!! 
I might just trash the rest of these so as not to contaminate my entire work space and wait for the dishes to come in.
-------------------- "You can peel it [language] off the ceiling and make it dance in front of you" - McKenna
<3 .
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