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Poor Boy
Fuck'O The Clown



Registered: 01/16/12
Posts: 7,473
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injection ports
#15757243 - 02/03/12 06:36 PM (12 years, 11 months ago) |
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how do you tell if the ports sealed all the way? im now extremely paranoid about the needle possible being too hot and not being sterile enough cause the needle was not red hot.
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Wise Toad


Registered: 06/08/10
Posts: 2,690
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Re: injection ports [Re: Poor Boy]
#15757334 - 02/03/12 06:56 PM (12 years, 11 months ago) |
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RTV right?
Iv never had a problem unless its like the 10th injection through the same port and some have survived longer than that
I don't know what to say about the red hot part, you have an alcohol lamp right? if you dont check my journal and the bottom link in my sig
What gauge are the needles, I have had some lower gauge needles(14/16 gauge) rip up my port
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Poor Boy
Fuck'O The Clown



Registered: 01/16/12
Posts: 7,473
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Re: injection ports [Re: Wise Toad]
#15757564 - 02/03/12 07:37 PM (12 years, 11 months ago) |
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they are from sporeworks. not sure what gauge they were. everyone seems to say the needle needed to be red hot, but i think it should be fine after hanging over the flame for a good 10 seconds.
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: injection ports [Re: Poor Boy]
#15757683 - 02/03/12 08:05 PM (12 years, 11 months ago) |
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To flame sterilize you need to ensure that the needle is RED HOT. This will kill all bacteria, molds, contaminate spores etc that are present both on and inside the needle, and if you are not waiting until your needle is red hot you are not doing it right.
As for the needle being hot when injecting into ports or RTV, the way to get around this is to gently allow a few drops of solution to pass into the needle and it will instantly cool down the needle enough to not burn whatever you are injecting into.
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Wise Toad


Registered: 06/08/10
Posts: 2,690
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forgot about that last part, as always good shit Evil; I do open air mostly when injecting jars, would you advise the cooling down protocol for such? Since your here Iv got a few questions for a pro
Its been a while and Im just getting back into it, cant remember if I used to cool the needle outside of the GB or not
Another thing, I always wipe the injection port down with alcohol; is that necessary?
Is there any reason to wear a surgical mask while working in a glove box?
and poor boy, are you talking about those blue stopper injection ports; I used to use those but they wore out quick, I either filled the hollow part with RTV silicone to extend their life or got rid of the lid all together in favor of a plain RTV silicone injection port
carry on gentlemen
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: injection ports [Re: Wise Toad]
#15757959 - 02/03/12 09:16 PM (12 years, 11 months ago) |
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If you are injecting BRF cakes or the like I would not worry about cooling your needle, only when injecting into jars with self healing ports would I worry about cooling the needle.
If you are using a glove box likely by the time you have your syringe back in the box and ready to inoculate the needle will have cooled enough.
Wiping a needle down after flame sterilizing is counter productive, nothing is more sterile then red hot, and you are opening a vector of contamination.
I wear a surgical mask when doing anything that needs to be sterile, when you breath you are releasing all sorts of organisms that can contaminate your work, its never a bad idea to wear a surgical mask when working.
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 8 days
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Quote:
EvilMushroom666 said: As for the needle being hot when injecting into ports or RTV, the way to get around this is to gently allow a few drops of solution to pass into the needle and it will instantly cool down the needle enough to not burn whatever you are injecting into.
Have you ever actually burned the port? I use the homemade silicone seals and always push it through while it's still red hot and haven't run into any problems.
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EvilMushroom666
Heretic




Registered: 11/18/09
Posts: 10,289
Loc: Canada
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Re: injection ports [Re: Kizzle]
#15758186 - 02/03/12 10:14 PM (12 years, 11 months ago) |
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I find that when using self healing injection ports and not cooling the needle it leaves a bigger opening after you pull it back out.
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deadmeadow
Stranger
Registered: 01/31/12
Posts: 81
Last seen: 11 years, 11 months
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Your injection port is probably fine.. Since you didn't get the needle all that hot and you wiped it, but as far contamination goes all you can do is wait patiently and see unfortunately.
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Wise Toad


Registered: 06/08/10
Posts: 2,690
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Quote:
EvilMushroom666 said: If you are using a glove box likely by the time you have your syringe back in the box and ready to inoculate the needle will have cooled enough.
Wiping a needle down after flame sterilizing is counter productive, nothing is more sterile then red hot, and you are opening a vector of contamination.
I meant working without a glove box, if your not in one do you still quick cool the needle
and I didnt mean wipe down the needle, wipe down the port that your about to stick the needle through or not?
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 8 days
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Re: injection ports [Re: Wise Toad]
#15758762 - 02/04/12 01:36 AM (12 years, 11 months ago) |
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I would wipe the port down first whether or not using a glovebox. Then if you're not using a box I would also wrap an alcohol soaked cotton ball or paper towel around the needle before pulling it out of the hole, and hold it there as you pull the needle out and a few seconds after so it can cover the hole until it reseals.
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Wise Toad


Registered: 06/08/10
Posts: 2,690
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Re: injection ports [Re: Kizzle]
#15758875 - 02/04/12 02:46 AM (12 years, 11 months ago) |
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I used to use the whole cotton ball/sponge thing but eventually just found it tedious, even on the way out Iv done nothing and have turned out fine; I have always alcohol wiped the port beforehand though, I dont know if that initial wipe is necessary or not?
I havent had a jar contam on me since my first(horrid) grow, I gotta keep it that way
I think I have always let the needle cool for several seconds without squirting a drop to quick cool when not using a glove box but I just cant remember
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Poor Boy
Fuck'O The Clown



Registered: 01/16/12
Posts: 7,473
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Re: injection ports [Re: Wise Toad]
#15759133 - 02/04/12 05:57 AM (12 years, 11 months ago) |
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should i re order?
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Montock
Time = $


Registered: 08/27/09
Posts: 1,838
Last seen: 11 years, 9 months
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Re: injection ports [Re: Poor Boy]
#15759375 - 02/04/12 08:38 AM (12 years, 11 months ago) |
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I use bernzomatic hand held propane torch for my box and hood work, you HAVE to squirt a drop out before using the port, I've yet to find a port, vendor or homemade that I can't destroy if I smoke to much and try to inj while still hawt. Red rtv silicone homeade ones stand the best chance against a red hawt needle tip, but won't last, look trashy . For bags you can inj red hot if you go old school and use duct tape , which works just as well as any port, if not better, and is literally more than 100x cheaper .
-------------------- Looking for Wife, please pm me your picture and list of skillz.
I'm wrong all the time, my posts are subject to being wrong at any point or time, get butt hurt at own risk.
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Poor Boy
Fuck'O The Clown



Registered: 01/16/12
Posts: 7,473
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Re: injection ports [Re: Montock]
#15759490 - 02/04/12 09:30 AM (12 years, 11 months ago) |
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my biggest concern is not getting the needle red hot...
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Breadnbutterfly86
Religion limits human potential

Registered: 02/06/10
Posts: 870
Loc: Wonderland
Last seen: 11 years, 2 months
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Re: injection ports [Re: Poor Boy]
#15759714 - 02/04/12 10:51 AM (12 years, 11 months ago) |
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no problems here with a flame to the needle for 7, 10 seconds or so and wiping the needle with alcohol afterwards... sterile and cools it. at least in these experiences
-------------------- "Don't just do something, stand there!" -Mr. Rabbit-
Breadnbutterfly
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 8 days
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Re: injection ports [Re: Wise Toad]
#15759958 - 02/04/12 12:07 PM (12 years, 11 months ago) |
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Quote:
Wise Toad said: I used to use the whole cotton ball/sponge thing but eventually just found it tedious, even on the way out Iv done nothing and have turned out fine; I have always alcohol wiped the port beforehand though, I dont know if that initial wipe is necessary or not?
I havent had a jar contam on me since my first(horrid) grow, I gotta keep it that way
I think I have always let the needle cool for several seconds without squirting a drop to quick cool when not using a glove box but I just cant remember
I've gotten away without it too and with letting the needle cool first for a few seconds. I don't it tedious at all though, usually have the alcohol wipe still sitting there from wiping the surface anyways. I figure in the long run I'm probably saving myself from some contamination but maybe I'm just being anal 
Poor Boy, making an alcohol lamp is extremely simple. It's basically a jar with a hole and a wick (piece of string or cotton) sticking out through the hole. You should consider making one and you won't have any problem getting the needle red hot.
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Wise Toad


Registered: 06/08/10
Posts: 2,690
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Re: injection ports [Re: Kizzle]
#15760114 - 02/04/12 12:51 PM (12 years, 11 months ago) |
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