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Offlinefraggy
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H2O2 or iodine?
    #15755615 - 02/03/12 11:44 AM (12 years, 11 months ago)

Hi all!

When it comes to cloning tissue, what would be the best to dunk your "cutting" in before dropping it on the agar or cardboard? H2O2 solution or iodine solution? And at what concentration should these be?

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Offlinefraggy
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Re: H2O2 or iodine? [Re: fraggy]
    #15756286 - 02/03/12 02:51 PM (12 years, 11 months ago)

And what about bleach?

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Offlinebishlap
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Re: H2O2 or iodine? [Re: fraggy]
    #15756330 - 02/03/12 03:05 PM (12 years, 11 months ago)

I don't recal dunking in steril solution as being a step in any cloning guide.
I think bore cloning would work great and you could cut off the exposed area leaving a clean sample.


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