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Offlinemrr0gers
A man with toomany sweaters...

Registered: 10/19/02
Posts: 113
Last seen: 11 years, 9 months
Outdoor G2G Transfer Experiment...
    #1575399 - 05/25/03 12:13 AM (14 years, 1 month ago)

Ok, about a week ago afoaf posted a question asking if it would be possible to make syringes outside considering that everything (syringe, water, print) were sterile. Afoaf asked this question because afoaf read somewhere that the reason outdoor cultivation is possible without contams is because airbourne spores is an indoor sorta thing. So just a few minutes ago afoaf decided to do a grain 2 grain transfer outside. Afoaf wiped down a teaspoon with 91% rubbing achohol and scooped out a teason of PF cake for each birdseed jar. Put the lids back on and shook the jars vigourously. Afoaf will post the results of this experiment as soon as the jars start colonizing...

P.S. Afoaf am stoned as of this writing and stoned when afoaf did the experiment.


--------------------
In loving memory of Fred Rogers.


Edited by mrr0gers (05/25/03 12:17 AM)


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Offlinefugu
Pooh-Bah

Registered: 01/06/03
Posts: 2,223
Loc: istanbul
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Re: Outdoor G2G Transfer Experiment... [Re: mrr0gers]
    #1575403 - 05/25/03 12:18 AM (14 years, 1 month ago)

dude..


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mushroom culture history making ...Mr. Allan is the best .....


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Offlinesi1988crx
Beep beep byebye

Registered: 03/08/03
Posts: 267
Last seen: 4 years, 6 months
Re: Outdoor G2G Transfer Experiment... [Re: fugu]
    #1575426 - 05/25/03 12:38 AM (14 years, 1 month ago)

Most likely you will fail. The reason contaminates happen inside is that there is an abundance of bad bacteria, and not enough good bacteria to balance out. If you open a sterlilized jar outside, the bad bacteria will get in and there wont be enough good bacteria to fight it off.

Good luck and maybe you will be lucky enough that the jars were not open long enough to get any spores in them.

By the way, when I said good and bad bacteria, any bacteria can be bad in high amounts. It is because there is a natural balance outdoors that will not let any one bacteria overrun another like it will indoors. If ANY bacteria get inside a sterillized jar it will multiply and over take the jar. Your best bet would to build a cheap flow hood or glove box and do your g2g transfers in there.


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Offlinemrr0gers
A man with toomany sweaters...

Registered: 10/19/02
Posts: 113
Last seen: 11 years, 9 months
Re: Outdoor G2G Transfer Experiment... [Re: si1988crx]
    #1575506 - 05/25/03 01:25 AM (14 years, 1 month ago)

Thank you for the info, CRX. 5 mushrooms for you!


--------------------
In loving memory of Fred Rogers.


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OfflineChemical_Smile
Making Love WithMy Ego
Registered: 09/20/01
Posts: 2,217
Loc: coming down fast, miles a...
Last seen: 13 years, 7 months
Re: Outdoor G2G Transfer Experiment... [Re: mrr0gers]
    #1576215 - 05/25/03 01:22 PM (14 years, 1 month ago)

Yeah, it doesnt sound like it would work that well. My understanding is that alot of the protective bacteria is in the soil and such but unless they are sitting in a hole you wont realy get these benefits, also the U.V. rays from the sun are supposed to help and you wont be outside long enough for that.


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OfflinePuffPuffGive
journeyman

Registered: 01/29/03
Posts: 54
Last seen: 13 years, 3 months
Re: Outdoor G2G Transfer Experiment... [Re: Chemical_Smile]
    #1576730 - 05/25/03 07:49 PM (14 years, 1 month ago)

afoaf does all his g2g transfers in the kitchen, just wipes everything with alchol and its all good.


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InvisibleOlgualion
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 User Gallery

Registered: 11/14/01
Posts: 1,253
Loc: Currently Earth...
Trusted Cultivator
Re: Outdoor G2G Transfer Experiment... [Re: mrr0gers]
    #1576745 - 05/25/03 08:07 PM (14 years, 1 month ago)

When dealing with grain, ANY bacteria or spores (except the type you are growing) are bad!

This whole good bacteria and bad bacteria thing only pertains to pasteurized substrates and casing, but never grain. Whenever doing anything with grain, cleanliness and sterility are critical to success.

I seriously doubt that your transfers will work, but I suppose anything is possible...


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Study the past...
See the future...


Edited by Olgualion (05/25/03 08:09 PM)


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OfflineChrisH
Dang ole' dang
Male

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Registered: 04/12/03
Posts: 112
Loc: DIrty south- come on
Last seen: 6 years, 4 months
Re: Outdoor G2G Transfer Experiment... [Re: Olgualion]
    #1577116 - 05/25/03 11:19 PM (14 years, 1 month ago)

I did the same thing except indoors with the oven tek and now have 10 jars from 1---with no contams.


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Are you sure you want to eat those?:mushroom2:


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