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OfflineShroomvroom
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Registered: 02/02/12
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Last seen: 12 years, 11 months
Experiment
    #15752550 - 02/02/12 05:12 PM (12 years, 11 months ago)

Ok so I have about 10 1/2 pint BRF jars going, and also have three 1/4 pint jars going. I made the 1/4 jars specifically to try new things with. After inoculation only one side of each jar germinated, so at this point in time (2 1/2 weeks later) 1 jar is about 70% colonized which I plan to let fully colonize for a different experiment, the other 2 jars are about 50% colonized but are going extremely slow (I think this is because the verm/brf mix is a little off). So basically what I would like to try to do is crumble these two cakes together in the same plastic bag and see if that will make them colonize faster, then I would just spawn. I was planning on doing this in the glove box and just opening the jars, dumping out as much of the excess verm layer I can into a bowl, then transfer and crumble the cakes to a ziplock freezer bag to finish colonizing. I read a post from RogerRabbit saying that ziplock bags come pretty sterile fresh out of the box, but I was thinking about adding gas exchange holes with micropore tape over them as I'm not even sure it would finish colonizing without them. Maybe sterilize a knife or some scissors and cut then immediately cover? I dunno I would like to see what you guys think, because I've been lurking around and I can tell you guys know your stuff.

Thanks.

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Invisibleslapphappypill
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Re: Experiment [Re: Shroomvroom]
    #15752573 - 02/02/12 05:18 PM (12 years, 11 months ago)

use a nail to pierce the zip lock and cover with the micropore right away... I'm not sure how crumbling letting them colonize in a ziplock will help if you plan to spawn right after though... might make more sense to just let the cake go, and then birth and crumble to bulk


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~

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OfflineShroomvroom
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Registered: 02/02/12
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Last seen: 12 years, 11 months
Re: Experiment [Re: slapphappypill]
    #15752584 - 02/02/12 05:20 PM (12 years, 11 months ago)

I mean it doesn't really make much sense, but I would like to see if the colonization speed increases just for future knowledge. I like the nail idea, how many holes do you think would suffice? My immediate guess would be about four.

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Invisibleslapphappypill
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Registered: 11/07/10
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Re: Experiment [Re: Shroomvroom]
    #15752611 - 02/02/12 05:28 PM (12 years, 11 months ago)

depending on the ziplock size, 4 should be just fine unless your messing with gallons


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~

Extras: Filter Print Post Top
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