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OfflineBlirska
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First monotub // inoculation question
    #15751900 - 02/02/12 02:14 PM (12 years, 11 months ago)

Good day kind sirs,

My first grow of cubes was a few year back using PF tek. While it was fun, I found it quite time consuming and would like to try a monotub.

I don't plan on doing multiple tubs as the yield of a single one will suffice me and my friends for a long time. Therefore, I'm not looking for the most efficient technique, but rather the quickest and easiest.

I'm planning on skipping LC. G2G would be an option, but since I don't mind using a whole syringe for a tub, could I juste inoculate multiple spawn jars directly? From my understanding, mycelium exchanges genetics whenever it fuses together. Therefore, multiple jars from the same syringe should be able to fuse together without a problem... but I'd still like to make sure.


Thanks :smile:

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InvisibleTranscendingLife
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Re: First monotub // inoculation question [Re: Blirska]
    #15751919 - 02/02/12 02:21 PM (12 years, 11 months ago)

here's my monotub tek.
https://www.shroomery.org/forums/showflat.php/Number/14507008#14507008

You can do a G2G or inoculate all your jars w/ MS from the same syringe or variety of PS.C.
Either way, you'll be fine w/ your monotub.
Only use 1cc of MS per quart jar.


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Invisibleslapphappypill
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Re: First monotub // inoculation question [Re: Blirska]
    #15751921 - 02/02/12 02:21 PM (12 years, 11 months ago)

it'll work fine, each jar though will have it's own genetics, just like separate cakes.

As long as it's cube mycelium, it'll mate with itself and keep growing


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I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~

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Offlineeutebius
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Re: First monotub // inoculation question [Re: slapphappypill]
    #15752012 - 02/02/12 02:55 PM (12 years, 11 months ago)

Yep,

What they said. TL's monotub protocol is sound; use Damion50/50's simple coir tek.

MS or MS-->G2G. The most common and "quickest and easiest" thing to do is to cover your ass a bit:
1) Multispore 1cc into 12-15+ Qt spawn jars.
2) Select the jar with the most robust myc, and G2G that into 12 other jars
3) With your remaining spawn jars [some will fail, but let's say you have 10 fully colonized] do your spawn run into coir. Then, you have a very high spawn-to-substrate ratio, which will colonize quickly. [RR, old man Agar, and TL, etc. are wonderfully right about this.]

Going for a first-time monotub, this allows you to hedge bets against potential problems.
Work clean.
Go.


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