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Offlinesubiedude
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Registered: 02/06/09
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LC lid quesion
    #15751071 - 02/02/12 09:49 AM (12 years, 11 months ago)

I'm making my lid for my first LC. I made a inoculation point using self healing glue, do I need to make a filter patch as well for air flow or gas exchange or just a inoculation point? How many cc's of multispore should i use for 8 0z worth of  malt extract(4%)/water?

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Invisibletruskool
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Registered: 06/28/09
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Re: LC lid quesion [Re: subiedude]
    #15751210 - 02/02/12 10:36 AM (12 years, 11 months ago)

A properly constructed lid will have both a SHIP(Self Healing Injection Port)  You said self healing glue? I have no clue what that is but it should be made out of RTV or silicone.  Some sort of hole that is able to provide filtered GE is needed.  I usually use .5-1 ml of spore solution for my LC's.


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OfflineShamrocker
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Re: LC lid quesion [Re: truskool]
    #15751238 - 02/02/12 10:44 AM (12 years, 11 months ago)

I use a half inch hole with a SFD attached with RTV for GE, though polyfill will also work providing it doesn't get wet

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Offlinesubiedude
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Re: LC lid quesion [Re: Shamrocker]
    #15751590 - 02/02/12 12:47 PM (12 years, 11 months ago)

thanks guys. so pretty much treat it like a jar of grain?:mushroom2:
How long does it take to see growth and when is best to use?

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InvisibleJavadog
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Re: LC lid quesion [Re: subiedude]
    #15751706 - 02/02/12 01:19 PM (12 years, 11 months ago)

:whattheysaid:  ;0)

If you do not have a GE port, then a vacuum will form inside the jar
and the instant you poke a syringe into the jar it's entire contents
will be sucked out.

Sounds crazy, but it has happened.

Good luck,

JD


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Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

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OfflineShamrocker
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Re: LC lid quesion [Re: Javadog]
    #15751971 - 02/02/12 02:41 PM (12 years, 11 months ago)

Oh it's happened all right! Lol

7 ccs of PE into one jar, half a cc into three others.

Oddly enough,the lean LCs far out performed the loaded one.

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Invisibletruskool
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Re: LC lid quesion [Re: Shamrocker]
    #15752646 - 02/02/12 05:38 PM (12 years, 11 months ago)

Quote:

Shamrocker said:
Oh it's happened all right! Lol

7 ccs of PE into one jar, half a cc into three others.

Oddly enough,the lean LCs far out performed the loaded one.



I have found that less is more with MS.  Less genetic competition leads to better faster growth IME.


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  Roll it :burnone:While I troll it.  :maximumtrolling:

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