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InvisibleDJYoshaBYD
Male

Registered: 04/07/07
Posts: 2,405
really annoying; nothing is growning
    #15750874 - 02/02/12 08:36 AM (12 years, 11 months ago)

ok, so I have done this shit HELLA times in my life, and for some reason, I am having nothing but issues.

first 6 jars of wbs, prepared like i always have.... nothing grew using an older spore syringe (some thai strain)..

SO, i got 24 more jars, prepared the wbs, and PCd at 15 psi for 60 minutes (again, not changing a SINGLE step in what I have done in the past)..

the first 12 jars got noc'd up with a syringe that I made using a spore print that didnt have too much left on it.. nothing grew in these jars, so I was thinking that it was an issue with spores..

I made a NEW spore syringe with a NEW, UNUSED, UNOPENED spore print from the same batch of spores that I used last year (z-strain).. The 2 syringes i made were LOADED with spores.. I let them sit overnight to hydrate..

I nocd up those 12 jars, and on the 3rd day, I started seeing little white fuzzies (yeaaahhh!!!), and this AM, 2 days later (5th day), these white spots are now turning green (FUCK!)..

I still have another VERY full spore syringe that I am going to try, but seriously, this is WAY fucking out of hand.. I have never had these issues.. not even when I first started using wbs as spawn..

Is it possible that the wbs I got was resistant in some way? like, a fungicide? it says no additives and all that on the bag.. I got the generic petco stuff.. I dont think its fungicide, because if it was, then even the trich would not be growing in there.. at least i wouldnt think so..

My sterile technique is the same, my wbs prep is the same (except im now using a 5 gallon bucket with a screen as a strainer), PC times PSI are the same, syringes made the same way, with the same set of prints that grew like wildfire last time....... I dont get wtf I am doing wrong..

any suggestions?

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Offlinecrimsondrac
Stranger
Registered: 12/09/11
Posts: 493
Last seen: 11 years, 8 months
Re: really annoying; nothing is growning [Re: DJYoshaBYD]
    #15750900 - 02/02/12 08:49 AM (12 years, 11 months ago)

Well, without more detail, It is going to be hard for even the experts to help you.  Are you soaking your WBS for 24 hours? Are you simmering it? do you let it dry out for a few hours? are you using quart jars? Also, you should be PCing it for 90 mins at 15psi. I think 60 mins. is not long enough.

Steps I take for WBS are:
1. Soak for 24 hours
2. Rinse until water is clear.
3. Simmer for 30 mins. (water steaming but no bubbles)
4. Strain and lay out on screen or towels for a few hours.
5. Load up into jars/Glad plastic containers.
6. Pressure cook for 90 mins at 15 psi
7. Let it all cool down naturally.
8. Wait 48 hours to see if anyhing grows in the WBS.
9. Innoc. and more waiting.

So far so good. Have not had anny issues.

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OfflineBig Pappa
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Registered: 09/03/11
Posts: 717
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Re: really annoying; nothing is growning [Re: DJYoshaBYD]
    #15750913 - 02/02/12 08:52 AM (12 years, 11 months ago)

start some spores on an agar plate to see if maybe your prints are dirty....

If not then transfer a wedge into 1 jar of WBS, then do transfers to you other jars after it colonizes...

Good luck, Ive been hit with the trich bomb myself and am still fighting it off!


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InvisibleDJYoshaBYD
Male

Registered: 04/07/07
Posts: 2,405
Re: really annoying; nothing is growning [Re: Big Pappa]
    #15750957 - 02/02/12 09:08 AM (12 years, 11 months ago)

well, i have listed my method before, which  isnt mine, but its always worked..

measure out WBS, pour into a bucket

get a couple of gallons of water almost boiling

pour into bucket over WBS, close, let sit for 24 hours

*at this point, the wbs is hydrated.. I can split it with my fingernail

strain wbs (yes. it was strained well.. no wet spot, no standing water in the jars after PC)

load jars, cover with tyvek and foil, PC for 60 minutes at 15 psi

let cool, noc up


again, this is the same method I have used HELLA times.. i have pics and grow logs to prove it.. im sure its not the wbs prep..

As for the agar and petri dishes, alllll of that is gone, as that is how I would have done it from the beginning.. some asshole narc'd me out a year ago, and I got rid of everything but my spores..

the only thing I can think of is a contam'd print/syringe like papa said.. my sterile technique is good, though.. glovebox and everything.. grrrrr.. hella annoying..

I guess I should just got get another bag of wbs, different brand, and do it again.. its just annoying that 100% of my jars are failing, and i have NEVER EVER had that high of a failure rate.. ive had REAL shitty luck lately (losing my job, about to get evicted), so I guess this is just following suit.. hahaha

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InvisibleDJYoshaBYD
Male

Registered: 04/07/07
Posts: 2,405
Re: really annoying; nothing is growning [Re: DJYoshaBYD]
    #15750982 - 02/02/12 09:17 AM (12 years, 11 months ago)

kind of a blurry pic.. done the same way, with prints from the same person.. 13 days from noc to colonized, from multispore.. very very aggressive..

this was a year ago..



now like, every jar I have noc'd up, with syringes from 2 different prints, are all either not growing, or showing trich.. maybe I need to bleach down my grow room or something? maybe something is just floating in the air?

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InvisibleHorizonSpawn
Gettin' my grow on :)
Male

Registered: 05/14/11
Posts: 1,739
Loc: Myco-Tek.org Flag
Re: really annoying; nothing is growning [Re: DJYoshaBYD]
    #15750996 - 02/02/12 09:23 AM (12 years, 11 months ago)

I agree with BigPappa; put some spores onto an agar plate and isolate away from contaminates :thumbup:


--------------------
Please assume any and all prints exchanged are "WILD" in nature; and thus, should NOT be considered ASEPTIC...

NOTE:  Please excuse my brevity, as it is a bitch 'n' a half and slow as hell to type on this here phone :frown:

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InvisibleDJYoshaBYD
Male

Registered: 04/07/07
Posts: 2,405
Re: really annoying; nothing is growning [Re: HorizonSpawn]
    #15751011 - 02/02/12 09:29 AM (12 years, 11 months ago)

like i said, i dont have petri dishes anymore.. otherwise I would have isolated.. i dont like MS nocs, and always isolate, grow out, and do g2g transfers..

but I am going to just start over.. I have another spore print that hasnt been used, so I think I will just noc up my jars in another part of the house, and bleach my whole grow room.. top to bottom.. lol..

Like i said, im just annoyed, as I have never ever ever had these issues... as stated previously, i think im just getting shit on by bad luck left and right.. hahaha

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InvisibleDJYoshaBYD
Male

Registered: 04/07/07
Posts: 2,405
Re: really annoying; nothing is growning [Re: DJYoshaBYD]
    #15751105 - 02/02/12 10:01 AM (12 years, 11 months ago)

arrrgg.. so I just went through all of the jars that I nocd up 5 days ago.. so far, 7 of them show trich, and the others show some white growth, but its too early to tell if its trich..

seriously, ill be happy if only 1 jar comes out.. then im just going to do g2g transfers.. come on, 1 jar!! hahaha.. thats all it will take to get this started..

needless to say, its very disheartening when you do something the same why a million times, and then it just stops working.. :frown:

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OfflineBig Pappa
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Registered: 09/03/11
Posts: 717
Loc: Smurf Village Flag
Last seen: 11 years, 18 days
Re: really annoying; nothing is growning [Re: DJYoshaBYD]
    #15752848 - 02/02/12 06:30 PM (12 years, 11 months ago)

surely you have an old 1/2 pint jar laying around somewhere you can make into a petri dish.... 

Just mix up some DME and agar, put it in there, PC it for an hour or so and presto...  great big petri dish!!!

If trich has settled in... thats the only thing Ive found to ensure clean jars...

Good luck!!!


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InvisibleDJYoshaBYD
Male

Registered: 04/07/07
Posts: 2,405
Re: really annoying; nothing is growning [Re: Big Pappa]
    #15755299 - 02/03/12 10:15 AM (12 years, 11 months ago)

word.. i just checked this am.. actually, 10 of the jars are showing myc growth and NO GREEN BASTARD! haha.. so, i guess i just jumped the gun.. the others are green, and have since been cleaned out.. I started the cleaning of my room yesterday, and am going to finish today, as well as get some more wbs, and start those 14 jars over.. so, yeah.. looks like its not a total loss.. im going laboratory technician from now on.. hahaha..

thanks for your responses..

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